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Literature summary extracted from

  • Bruckmann, M.; Blasco, R.; Timmis, K.N.; Pieper, D.H.
    Detoxification of protoanemonin by dienelactone hydrolase (1998), J. Bacteriol., 180, 400-402.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.45 protoanemonin competitive inhibitor of the transformation of cis-dienelactone Pseudomonas knackmussii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.45 0.381
-
 cis-4-carboxymethylenebut-2-en-4-olide
-
 Pseudomonas knackmussii
3.1.1.45 0.415
-
 protoanemonin
-
 Pseudomonas knackmussii
3.1.1.45 1.8
-
 trans-4-Carboxymethylenebut-2-en-4-olide
-
 Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.45 additional information Burkholderia cepacia
-
 ?
-
 ?
3.1.1.45 additional information Pseudomonas knackmussii it seems likely, that dienelactone hydrolase has the additional function of detoxification of minor amounts of protoanemonin that may be formed during chloroaromatic degradation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.45 Burkholderia cepacia
-
 Pseudomonas cepacia
-
3.1.1.45 Pseudomonas knackmussii
-
-
-
3.1.1.45 Pseudomonas putida
-
 RW10, trans-dienelactone hydrolase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.45 3-chlorobenzoate-grown cells Pseudomonas knackmussii
3.1.1.45 partial Burkholderia cepacia
3.1.1.45 RW10 partial Pseudomonas putida

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.45 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate the active-site Cys123 residue is part of a triad of residues consisting of Cys123, His202 and Asp171, and is reminiscent of the serine/cysteine proteases Pseudomonas knackmussii
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.45 0.58
-
 substrate protoanemonin Pseudomonas knackmussii
3.1.1.45 58.8
-
 substrate cis-4-carboxymethylenebut-2-en-4-olide Pseudomonas knackmussii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Burkholderia cepacia 4-oxohex-2-enedioate
-
 ?
3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?
3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O Pseudomonas putida RW10 not a substrate to a significant extent Pseudomonas putida 4-oxohex-2-enedioate
-
 ?
3.1.1.45 additional information
-
 Burkholderia cepacia ?
-
 ?
3.1.1.45 additional information it seems likely, that dienelactone hydrolase has the additional function of detoxification of minor amounts of protoanemonin that may be formed during chloroaromatic degradation Pseudomonas knackmussii ?
-
 ?
3.1.1.45 protoanemonin + H2O
-
 Pseudomonas knackmussii cis-acetylacrylate
-
 ?
3.1.1.45 protoanemonin + H2O activity of cis-dienelactone hydrolase with 50 microM protoanemonin is 0.08% of that observed with 50 microM cis-dienelactone, cis-4-carboxymethylenebut-2-en-4-olide Burkholderia cepacia cis-acetylacrylate
-
 ?
3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas putida 4-oxohex-2-enedioate
-
 ?
3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.45 monomer
-
 Pseudomonas putida
3.1.1.45 monomer
-
 Burkholderia cepacia
3.1.1.45 monomer 1 * 28000-29000, SDS-PAGE Pseudomonas knackmussii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.45 2.08
-
 protoanemonin
-
 Pseudomonas knackmussii
3.1.1.45 260
-
 cis-4-carboxymethylenebut-2-en-4-olide
-
 Pseudomonas knackmussii