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Literature summary extracted from

  • Ngai, K.L.; Schlömann, M.; Knackmuss, H.J.; Ornston, L.N.
    Dienelactone hydrolase from Pseudomonas sp. strain B13 (1987), J. Bacteriol., 169, 699-703.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.45 additional information no requirement Pseudomonas knackmussii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.45
-
 Pseudomonas knackmussii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.45 p-chloromercuribenzoate inactivation completely reversed by dithiothreitol Pseudomonas knackmussii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.45 0.015
-
 trans-4-Carboxymethylenebut-2-en-4-olide
-
 Pseudomonas knackmussii
3.1.1.45 0.4
-
 cis-4-carboxymethylenebut-2-en-4-olide
-
 Pseudomonas knackmussii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.45 cytosol
-
 Pseudomonas knackmussii 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.45 additional information no requirement Pseudomonas knackmussii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.45 28500
-
 gel filtration and nondenaturing PAGE Pseudomonas knackmussii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.45 4-carboxymethylenebut-2-en-4-olide + H2O Pseudomonas knackmussii dienelactone hydrolase catalyzes a step in the metabolic conversion of chlorocatechols to beta-ketoadipate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.45 Pseudomonas knackmussii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.45 3-chlorobenzoate-grown cells Pseudomonas knackmussii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.45 65
-
-
 Pseudomonas knackmussii

Storage Stability

EC Number Storage Stability Organism
3.1.1.45 4°C, 20 mM Tris-HCl, pH 7.4, 10 micromol dithiothreitol, stable for over 6 months Pseudomonas knackmussii
3.1.1.45 4°C, converted to microcrystals, ammonium sulfate to 30% saturation, stable for more than 1 year Pseudomonas knackmussii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.45 4-carboxymethylenebut-2-en-4-olide + H2O dienelactone hydrolase catalyzes a step in the metabolic conversion of chlorocatechols to beta-ketoadipate Pseudomonas knackmussii ?
-
 ?
3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?
3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.45 monomer 1* 28000, SDS-PAGE with mercaptoethanol Pseudomonas knackmussii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.45 30
-
 4-carboxymethylenebut-2-en-4-olide
-
 Pseudomonas knackmussii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.45 7.5
-
-
 Pseudomonas knackmussii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.45 5 9
-
 Pseudomonas knackmussii