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Literature summary extracted from
- Purification and properties of a membrane-bound phospholipase A1 from Mycobacterium phlei (1974), J. Biol. Chem., 249, 5658-5667.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.32 | Triton X-100 | increases activity | Mycolicibacterium phlei |  |
| 3.1.1.32 | Triton X-100 | 0.05% w/v causes maximal enhancement of activity | Mycolicibacterium phlei | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.32 | Ca2+ | 10 mM, 20-60% increase in activity | Mycolicibacterium phlei | |
| 3.1.1.32 | EDTA | 10 mM, 30-40% inhibition | Mycolicibacterium phlei | |
| 3.1.1.32 | Fe2+ | 5 mM | Mycolicibacterium phlei | |
| 3.1.1.32 | Fe3+ | 1 mM, almost complete inhibition | Mycolicibacterium phlei | |
| 3.1.1.32 | Hg2+ | 5 mM | Mycolicibacterium phlei | |
| 3.1.1.32 | lauryldimethylbenzyl ammonium chloride | - |
Mycolicibacterium phlei | |
| 3.1.1.32 | SDS | - |
Mycolicibacterium phlei | |
| 3.1.1.32 | sodium deoxycholate | - |
Mycolicibacterium phlei | |
| 3.1.1.32 | Triton X-100 | at high concentration | Mycolicibacterium phlei | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.32 | plasma membrane | tightly bound | Mycolicibacterium phlei | 5886 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.32 | Mycolicibacterium phlei | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.32 | - |
Mycolicibacterium phlei |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.32 | additional information | - |
- |
Mycolicibacterium phlei |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.1.32 | -20°C, stable for at least 1 year | Mycolicibacterium phlei |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.32 | cardiolipin + H2O | - |
Mycolicibacterium phlei | ? | - |
? | |
| 3.1.1.32 | phosphatidylcholine + H2O | - |
Mycolicibacterium phlei | ? | - |
? | |
| 3.1.1.32 | phosphatidylethanolamine + H2O | - |
Mycolicibacterium phlei | ? | - |
? | |
| 3.1.1.32 | phosphatidylglycerol + H2O | - |
Mycolicibacterium phlei | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.32 | 3.7 | - |
hydrolysis of cardiolipin | Mycolicibacterium phlei |
| 3.1.1.32 | 4.5 | - |
hydrolysis of phosphatidylglycerol | Mycolicibacterium phlei |
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Release 2023.1 (January 2023)
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