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Literature summary extracted from
- Purification and properties of a lipolytic acyl-hydrolase from potato leaves (1979), Biochim. Biophys. Acta, 573, 155-165.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.26 | EDTA | 113% activity at 0.1 mM, 140% activity at 10 mM | Solanum tuberosum |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.26 | CaCl2 | no inhibition at 0.1 mM, 13% inhibition at 1 mM, 47% inhibition at 10 mM | Solanum tuberosum | |
| 3.1.1.26 | MgCl2 | 53% inhibition at 10 mM | Solanum tuberosum | |
| 3.1.1.26 | NaCl | 84% inhibition at 50 mM | Solanum tuberosum | |
| 3.1.1.26 | SDS | 0.1% complete inhibition | Solanum tuberosum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.26 | 0.26 | - |
monogalactosyldiacylglycerol | - |
Solanum tuberosum | |
| 3.1.1.26 | 0.54 | - |
phosphatidylcholine | - |
Solanum tuberosum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.26 | 110000 | - |
SDS-PAGE, gel filtration | Solanum tuberosum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.26 | digalactosyldiacylglycerol + H2O | Solanum tuberosum | - |
? | - |
? | |
| 3.1.1.26 | monogalactosyldiacylglycerol + H2O | Solanum tuberosum | - |
? | - |
? | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 3.1.1.26 | Acetone | 85% inhibition at 10% | Solanum tuberosum |
| 3.1.1.26 | Ethanol | 98% inhibition at 10% | Solanum tuberosum |
| 3.1.1.26 | ether | 96% inhibition at 10% | Solanum tuberosum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.26 | Solanum tuberosum | - |
L. cv. Benimaru | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.26 | 520fold | Solanum tuberosum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.26 | leaf | - |
Solanum tuberosum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.26 | 2,3-di-O-acyl-1-O-(beta-D-galactosyl)-D-glycerol + H2O | - |
Solanum tuberosum | ? | - |
? | |
| 3.1.1.26 | digalactosyldiacylglycerol + H2O | 44% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | digalactosylglycerol + fatty acid | - |
? | |
| 3.1.1.26 | digalactosyldiacylglycerol + H2O | - |
Solanum tuberosum | ? | - |
? | |
| 3.1.1.26 | digalactosylmonoacylglycerol + H2O | 146% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | digalactosylglycerol + fatty acid | - |
? | |
| 3.1.1.26 | lysophosphatidylcholine + H2O | 26% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | ? | - |
? | |
| 3.1.1.26 | lysophosphatidylethanolamine + H2O | 6% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | ? | - |
? | |
| 3.1.1.26 | lysophosphatidylglycerol + H2O | 89% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | ? | - |
? | |
| 3.1.1.26 | monogalactosyldiacylglycerol + H2O | - |
Solanum tuberosum | galactosylglycerol + fatty acid | - |
? | |
| 3.1.1.26 | monogalactosyldiacylglycerol + H2O | - |
Solanum tuberosum | ? | - |
? | |
| 3.1.1.26 | monogalactosylmonoacylglycerol + H2O | 394% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | monogalactosylglycerol + fatty acid | - |
? | |
| 3.1.1.26 | phosphatidic acid + H2O | 8% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | ? | - |
? | |
| 3.1.1.26 | phosphatidylcholine + H2O | 14% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | ? | - |
? | |
| 3.1.1.26 | phosphatidylethanolamine + H2O | 1% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | ? | - |
? | |
| 3.1.1.26 | phosphatidylglycerol + H2O | 77% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | ? | - |
? | |
| 3.1.1.26 | sulfoquinovosyldiaclglycerol + H2O | 43% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | ? | - |
? | |
| 3.1.1.26 | triacylglycerol + H2O | below 0.1% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.26 | lipid acyl-hydrolase | - |
Solanum tuberosum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.26 | 30 | - |
phosphatidylcholine as substrate | Solanum tuberosum |
| 3.1.1.26 | 35 | - |
monogalactosyldiacylglycerol as substrate | Solanum tuberosum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.26 | 60 | - |
completely inactivated after 10 min at pH 8.0, 70% of its activity retained at pH 5.5 | Solanum tuberosum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.26 | 5.5 | - |
monogalactosyldiacylglycerol as substrate, at 35°C, shift to pH 6.0 in the presence of Triton X-100 | Solanum tuberosum |
| 3.1.1.26 | 6 | - |
monogalactosyldiacylglycerol as substrate, at 35°C, shift from pH 5.5 in the presence of Triton X-100 | Solanum tuberosum |
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Release 2023.1 (January 2023)
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