BRENDA - Enzyme Database

Purification and properties of a lipolytic acyl-hydrolase from potato leaves

Matsuda, H.; Hirayama, O.; Biochim. Biophys. Acta 573, 155-165 (1979)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.26
EDTA
113% activity at 0.1 mM, 140% activity at 10 mM
Solanum tuberosum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.26
CaCl2
no inhibition at 0.1 mM, 13% inhibition at 1 mM, 47% inhibition at 10 mM
Solanum tuberosum
3.1.1.26
MgCl2
53% inhibition at 10 mM
Solanum tuberosum
3.1.1.26
NaCl
84% inhibition at 50 mM
Solanum tuberosum
3.1.1.26
SDS
0.1% complete inhibition
Solanum tuberosum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.26
0.26
-
monogalactosyldiacylglycerol
-
Solanum tuberosum
3.1.1.26
0.54
-
phosphatidylcholine
-
Solanum tuberosum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.26
110000
-
SDS-PAGE, gel filtration
Solanum tuberosum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.26
digalactosyldiacylglycerol + H2O
Solanum tuberosum
-
?
-
-
-
3.1.1.26
monogalactosyldiacylglycerol + H2O
Solanum tuberosum
-
?
-
-
-
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
3.1.1.26
Acetone
85% inhibition at 10%
Solanum tuberosum
3.1.1.26
Ethanol
98% inhibition at 10%
Solanum tuberosum
3.1.1.26
ether
96% inhibition at 10%
Solanum tuberosum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.26
Solanum tuberosum
-
L. cv. Benimaru
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.26
520fold
Solanum tuberosum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.26
leaf
-
Solanum tuberosum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.26
2,3-di-O-acyl-1-O-(beta-D-galactosyl)-D-glycerol + H2O
-
94293
Solanum tuberosum
?
-
-
-
?
3.1.1.26
digalactosyldiacylglycerol + H2O
44% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
digalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
digalactosyldiacylglycerol + H2O
-
94293
Solanum tuberosum
?
-
-
-
-
3.1.1.26
digalactosylmonoacylglycerol + H2O
146% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
digalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
lysophosphatidylcholine + H2O
26% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
3.1.1.26
lysophosphatidylethanolamine + H2O
6% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
3.1.1.26
lysophosphatidylglycerol + H2O
89% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
3.1.1.26
monogalactosyldiacylglycerol + H2O
-
94293
Solanum tuberosum
galactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
monogalactosyldiacylglycerol + H2O
-
94293
Solanum tuberosum
?
-
-
-
-
3.1.1.26
monogalactosylmonoacylglycerol + H2O
394% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
monogalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
phosphatidic acid + H2O
8% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
3.1.1.26
phosphatidylcholine + H2O
14% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
3.1.1.26
phosphatidylethanolamine + H2O
1% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
3.1.1.26
phosphatidylglycerol + H2O
77% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
3.1.1.26
sulfoquinovosyldiaclglycerol + H2O
43% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
3.1.1.26
triacylglycerol + H2O
below 0.1% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.1.26
30
-
phosphatidylcholine as substrate
Solanum tuberosum
3.1.1.26
35
-
monogalactosyldiacylglycerol as substrate
Solanum tuberosum
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.26
60
-
completely inactivated after 10 min at pH 8.0, 70% of its activity retained at pH 5.5
Solanum tuberosum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.26
5.5
-
monogalactosyldiacylglycerol as substrate, at 35°C, shift to pH 6.0 in the presence of Triton X-100
Solanum tuberosum
3.1.1.26
6
-
monogalactosyldiacylglycerol as substrate, at 35°C, shift from pH 5.5 in the presence of Triton X-100
Solanum tuberosum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.26
EDTA
113% activity at 0.1 mM, 140% activity at 10 mM
Solanum tuberosum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.26
CaCl2
no inhibition at 0.1 mM, 13% inhibition at 1 mM, 47% inhibition at 10 mM
Solanum tuberosum
3.1.1.26
MgCl2
53% inhibition at 10 mM
Solanum tuberosum
3.1.1.26
NaCl
84% inhibition at 50 mM
Solanum tuberosum
3.1.1.26
SDS
0.1% complete inhibition
Solanum tuberosum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.26
0.26
-
monogalactosyldiacylglycerol
-
Solanum tuberosum
3.1.1.26
0.54
-
phosphatidylcholine
-
Solanum tuberosum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.26
110000
-
SDS-PAGE, gel filtration
Solanum tuberosum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.26
digalactosyldiacylglycerol + H2O
Solanum tuberosum
-
?
-
-
-
3.1.1.26
monogalactosyldiacylglycerol + H2O
Solanum tuberosum
-
?
-
-
-
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
3.1.1.26
Acetone
85% inhibition at 10%
Solanum tuberosum
3.1.1.26
Ethanol
98% inhibition at 10%
Solanum tuberosum
3.1.1.26
ether
96% inhibition at 10%
Solanum tuberosum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.26
520fold
Solanum tuberosum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.26
leaf
-
Solanum tuberosum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.26
2,3-di-O-acyl-1-O-(beta-D-galactosyl)-D-glycerol + H2O
-
94293
Solanum tuberosum
?
-
-
-
?
3.1.1.26
digalactosyldiacylglycerol + H2O
44% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
digalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
digalactosyldiacylglycerol + H2O
-
94293
Solanum tuberosum
?
-
-
-
-
3.1.1.26
digalactosylmonoacylglycerol + H2O
146% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
digalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
lysophosphatidylcholine + H2O
26% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
3.1.1.26
lysophosphatidylethanolamine + H2O
6% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
3.1.1.26
lysophosphatidylglycerol + H2O
89% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
3.1.1.26
monogalactosyldiacylglycerol + H2O
-
94293
Solanum tuberosum
galactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
monogalactosyldiacylglycerol + H2O
-
94293
Solanum tuberosum
?
-
-
-
-
3.1.1.26
monogalactosylmonoacylglycerol + H2O
394% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
monogalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
phosphatidic acid + H2O
8% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
3.1.1.26
phosphatidylcholine + H2O
14% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
3.1.1.26
phosphatidylethanolamine + H2O
1% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
3.1.1.26
phosphatidylglycerol + H2O
77% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
3.1.1.26
sulfoquinovosyldiaclglycerol + H2O
43% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
3.1.1.26
triacylglycerol + H2O
below 0.1% of the activity with monogalactosyldiacylglycerol
94293
Solanum tuberosum
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.1.26
30
-
phosphatidylcholine as substrate
Solanum tuberosum
3.1.1.26
35
-
monogalactosyldiacylglycerol as substrate
Solanum tuberosum
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.26
60
-
completely inactivated after 10 min at pH 8.0, 70% of its activity retained at pH 5.5
Solanum tuberosum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.26
5.5
-
monogalactosyldiacylglycerol as substrate, at 35°C, shift to pH 6.0 in the presence of Triton X-100
Solanum tuberosum
3.1.1.26
6
-
monogalactosyldiacylglycerol as substrate, at 35°C, shift from pH 5.5 in the presence of Triton X-100
Solanum tuberosum