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Literature summary extracted from
- Enzymatic properties of a galactolipase from rice bran (1979), Agric. Biol. Chem., 43, 697-703.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.26 | 2-mercaptoethanol | traces of activity remain with 10 mM at pH 7.5 | Oryza sativa |  |
| 3.1.1.26 | CaCl2 | 79% inhibition at a concentration of 10 mM | Oryza sativa | |
| 3.1.1.26 | cetyltrimethylammonium chloride | 65% inhibition at 1 mM | Oryza sativa | |
| 3.1.1.26 | cysteine | traces of activity remain with 100 mM at pH 7.5 | Oryza sativa | |
| 3.1.1.26 | diisopropylfluorophosphate | 7% remaining activity with 1 mM at pH 7.5 | Oryza sativa | |
| 3.1.1.26 | EDTA | no inhibition at 1 mM, 78% inhibition at 10 mM | Oryza sativa | |
| 3.1.1.26 | additional information | not affected by 0.3 mM p-chloromercuribenzoate, and 30 mM N-ethylmaleimide | Oryza sativa | |
| 3.1.1.26 | SDS | 0.1% complete inhibition | Oryza sativa | |
| 3.1.1.26 | Triton X-100 | 31% inhibition at 0.1%, 83% inhibition at 1% | Oryza sativa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.26 | 0.34 | - |
monogalactosyldiacylglycerol | - |
Oryza sativa | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.26 | CaCl2 | 124% activity compared to assay without additives at 0.1 mM | Oryza sativa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.26 | 40000 | - |
gel filtration | Oryza sativa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.26 | digalactosyldiacylglycerol + H2O | Oryza sativa | - |
? | - |
? | |
| 3.1.1.26 | monogalactosyldiacylglycerol + H2O | Oryza sativa | - |
? | - |
? | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 3.1.1.26 | Acetone | complete inhibition at 1% | Oryza sativa |
| 3.1.1.26 | Ethanol | complete inhibition at 1% | Oryza sativa |
| 3.1.1.26 | ether | complete inhibition at 1% | Oryza sativa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.26 | Oryza sativa | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.26 | leaf | - |
Oryza sativa | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.26 | digalactosyldiacylglycerol + H2O | 169% of the activity with monogalactosyldiacylglycerol | Oryza sativa | digalactosylglycerol + fatty acid | - |
? | |
| 3.1.1.26 | digalactosyldiacylglycerol + H2O | - |
Oryza sativa | ? | - |
? | |
| 3.1.1.26 | lysophosphatidylcholine + H2O | 33% of the activity with monogalactosyldiacylglycerol | Oryza sativa | ? | - |
? | |
| 3.1.1.26 | monogalactosyldiacylglycerol + H2O | - |
Oryza sativa | galactosylglycerol + fatty acid | - |
? | |
| 3.1.1.26 | monogalactosyldiacylglycerol + H2O | - |
Oryza sativa | ? | - |
? | |
| 3.1.1.26 | monogalactosylmonoacylglycerol + H2O | 111% of the activity with monogalactosyldiacylglycerol | Oryza sativa | monogalactosylglycerol + fatty acid | - |
? | |
| 3.1.1.26 | phosphatidylcholine + H2O | 27% of the activity with monogalactosyldiacylglycerol | Oryza sativa | ? | - |
? | |
| 3.1.1.26 | phosphatidylethanolamine + H2O | traces of the activity with monogalactosyldiacylglacerol | Oryza sativa | ? | - |
? | |
| 3.1.1.26 | triacylglycerol + H2O | traces of the activity with monogalactosyldiacylglycerol | Oryza sativa | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.26 | 35 | - |
- |
Oryza sativa |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.26 | - |
45 | 0°C: 50% of the maximal activity, 45°C: 40% of the maximal activity | Oryza sativa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.26 | 50 | - |
incubation for 10 min at pH 7.5, 95% loss of activity, retains more thant 60% of its activity at pH 5.5 | Oryza sativa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.26 | 7.5 | - |
Tris-HCl buffer | Oryza sativa |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.26 | 4 | 9 | pH 4: 25% of the maximal activity, pH 9: 50% of the maximal activity | Oryza sativa |
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