BRENDA - Enzyme Database

Molecular weights of mitochondrial and cytoplasmic aminoacyl-tRNA synthetases of beef liver and their complexes

Walker, E.J.; Treacy, G.B.; Jeffrey, P.D.; Biochemistry 22, 1934-1941 (1983)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.15
55
-
4-selenaproline
ATP-diphosphate exchange
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.1.1.3
cytoplasm
-
Bos taurus
5737
-
6.1.1.3
mitochondrion
-
Bos taurus
5739
-
6.1.1.7
cytoplasm
-
Bos taurus
5737
-
6.1.1.7
mitochondrion
-
Bos taurus
5739
-
6.1.1.11
cytoplasm
-
Bos taurus
5737
-
6.1.1.11
mitochondrion
-
Bos taurus
5739
-
6.1.1.12
cytoplasm
-
Bos taurus
5737
-
6.1.1.14
cytoplasm
-
Bos taurus
5737
-
6.1.1.14
mitochondrion
-
Bos taurus
5739
-
6.1.1.15
cytoplasm
-
Bos taurus
5737
-
6.1.1.15
mitochondrion
-
Bos taurus
5739
-
6.1.1.16
cytoplasm
-
Bos taurus
5737
-
6.1.1.16
mitochondrion
-
Bos taurus
5739
-
6.1.1.17
cytoplasm
-
Bos taurus
5737
-
6.1.1.18
mitochondrion
-
Bos taurus
5739
-
6.1.1.21
cytoplasm
-
Bos taurus
5737
-
6.1.1.21
mitochondrion
-
Bos taurus
5739
-
6.1.1.22
cytoplasm
-
Bos taurus
5737
-
6.1.1.22
mitochondrion
-
Bos taurus
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.3
195000
-
mitochondrial, gel filtration
Bos taurus
6.1.1.3
206000
-
cytoplasmic, gel filtration
Bos taurus
6.1.1.7
141000
-
gel filtration, cytoplasmic enzyme
Bos taurus
6.1.1.7
162000
-
gel filtration, mitochondrial enzyme
Bos taurus
6.1.1.11
additional information
-
-
Bos taurus
6.1.1.12
117000
-
gel filtration, also a smaller peak of MW 57000 detected
Bos taurus
6.1.1.14
189000
-
gel filtration, mitochondrial enzyme
Bos taurus
6.1.1.14
202000
-
gel filtration, cytoplasmic enzyme
Bos taurus
6.1.1.15
155000
-
cytoplasmic, gel filtration
Bos taurus
6.1.1.15
162000
-
mitochondrial, gel filtration
Bos taurus
6.1.1.16
82000
-
gel filtration, mitochondrial enzyme
Bos taurus
6.1.1.16
262000
-
gel filtration, cytoplasmic enzyme
Bos taurus
6.1.1.17
111000
-
gel filtration
Bos taurus
6.1.1.18
138000
-
gel filtration
Bos taurus
6.1.1.21
153000
-
gel filtration, cytoplasmic
Bos taurus
6.1.1.21
154000
-
gel filtration, mitochondrial
Bos taurus
6.1.1.22
additional information
-
existence of high molecular weight aminoacyl-tRNA synthetase complexes within the mitochondria and the cytoplasm
Bos taurus
6.1.1.22
175000
-
cytoplasmic enzyme, dissociated activity arises from high molecular weight aminoacyl-tRNA synthetase complex, gel filtration
Bos taurus
6.1.1.22
189000
-
mitochondrial enzyme, dissociated activity arises from high molecular weight aminoacyl-tRNA synthetase complex, gel filtration
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.7
ATP + alanine + tRNAAla
Bos taurus
key role in protein biosynthesis
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.3
Bos taurus
-
-
-
6.1.1.7
Bos taurus
-
-
-
6.1.1.11
Bos taurus
-
multienzyme complex containing seryl-tRNA synthetase activity
-
6.1.1.12
Bos taurus
-
-
-
6.1.1.14
Bos taurus
-
-
-
6.1.1.15
Bos taurus
-
-
-
6.1.1.16
Bos taurus
-
-
-
6.1.1.17
Bos taurus
-
-
-
6.1.1.18
Bos taurus
-
-
-
6.1.1.21
Bos taurus
-
-
-
6.1.1.22
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.1.1.7
partial, from a multienzyme complex containing at least 16 aminoacyl-tRNA synthetase activities
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
6.1.1.3
liver
-
Bos taurus
-
6.1.1.7
liver
-
Bos taurus
-
6.1.1.11
liver
-
Bos taurus
-
6.1.1.12
liver
-
Bos taurus
-
6.1.1.14
liver
-
Bos taurus
-
6.1.1.16
liver
-
Bos taurus
-
6.1.1.17
liver
-
Bos taurus
-
6.1.1.18
liver
-
Bos taurus
-
6.1.1.21
liver
-
Bos taurus
-
6.1.1.22
liver
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.3
ATP + L-threonine + tRNAThr
-
92
Bos taurus
AMP + diphosphate + L-threonyl-tRNAThr
-
92
Bos taurus
-
6.1.1.7
ATP + alanine + tRNAAla
key role in protein biosynthesis
92
Bos taurus
?
-
-
-
-
6.1.1.7
ATP + L-alanine + tRNAAla
-
92
Bos taurus
AMP + diphosphate + L-alanyl-tRNAAla
-
92
Bos taurus
-
6.1.1.11
ATP + L-serine + tRNASer
-
92
Bos taurus
AMP + diphosphate + L-seryl-tRNASer
-
92
Bos taurus
-
6.1.1.12
ATP + L-aspartate + tRNAAsp
-
92
Bos taurus
AMP + diphosphate + L-aspartyl-tRNAAsp
-
92
Bos taurus
-
6.1.1.14
ATP + glycine + tRNAGly
-
92
Bos taurus
AMP + diphosphate + glycyl-tRNAGly
-
92
Bos taurus
-
6.1.1.15
ATP + L-proline + tRNAPro
-
92
Bos taurus
AMP + diphosphate + L-prolyl-tRNAPro
-
92
Bos taurus
-
6.1.1.16
ATP + L-cysteine + tRNACys
-
92
Bos taurus
AMP + diphosphate + L-cysteinyl-tRNACys
-
92
Bos taurus
-
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
92
Bos taurus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
92
Bos taurus
-
6.1.1.18
ATP + L-glutamine + tRNAGln
-
92
Bos taurus
AMP + diphosphate + L-glutaminyl-tRNAGln
-
92
Bos taurus
-
6.1.1.21
ATP + L-histidine + tRNAHis
-
92
Bos taurus
AMP + diphosphate + L-histidyl-tRNAHis
-
92
Bos taurus
-
6.1.1.22
ATP + L-asparagine + tRNAAsn
-
92
Bos taurus
AMP + diphosphate + L-asparaginyl-tRNAAsn
-
92
Bos taurus
-
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.15
55
-
4-selenaproline
ATP-diphosphate exchange
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.1.1.3
cytoplasm
-
Bos taurus
5737
-
6.1.1.3
mitochondrion
-
Bos taurus
5739
-
6.1.1.7
cytoplasm
-
Bos taurus
5737
-
6.1.1.7
mitochondrion
-
Bos taurus
5739
-
6.1.1.11
cytoplasm
-
Bos taurus
5737
-
6.1.1.11
mitochondrion
-
Bos taurus
5739
-
6.1.1.12
cytoplasm
-
Bos taurus
5737
-
6.1.1.14
cytoplasm
-
Bos taurus
5737
-
6.1.1.14
mitochondrion
-
Bos taurus
5739
-
6.1.1.15
cytoplasm
-
Bos taurus
5737
-
6.1.1.15
mitochondrion
-
Bos taurus
5739
-
6.1.1.16
cytoplasm
-
Bos taurus
5737
-
6.1.1.16
mitochondrion
-
Bos taurus
5739
-
6.1.1.17
cytoplasm
-
Bos taurus
5737
-
6.1.1.18
mitochondrion
-
Bos taurus
5739
-
6.1.1.21
cytoplasm
-
Bos taurus
5737
-
6.1.1.21
mitochondrion
-
Bos taurus
5739
-
6.1.1.22
cytoplasm
-
Bos taurus
5737
-
6.1.1.22
mitochondrion
-
Bos taurus
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.3
195000
-
mitochondrial, gel filtration
Bos taurus
6.1.1.3
206000
-
cytoplasmic, gel filtration
Bos taurus
6.1.1.7
141000
-
gel filtration, cytoplasmic enzyme
Bos taurus
6.1.1.7
162000
-
gel filtration, mitochondrial enzyme
Bos taurus
6.1.1.11
additional information
-
-
Bos taurus
6.1.1.12
117000
-
gel filtration, also a smaller peak of MW 57000 detected
Bos taurus
6.1.1.14
189000
-
gel filtration, mitochondrial enzyme
Bos taurus
6.1.1.14
202000
-
gel filtration, cytoplasmic enzyme
Bos taurus
6.1.1.15
155000
-
cytoplasmic, gel filtration
Bos taurus
6.1.1.15
162000
-
mitochondrial, gel filtration
Bos taurus
6.1.1.16
82000
-
gel filtration, mitochondrial enzyme
Bos taurus
6.1.1.16
262000
-
gel filtration, cytoplasmic enzyme
Bos taurus
6.1.1.17
111000
-
gel filtration
Bos taurus
6.1.1.18
138000
-
gel filtration
Bos taurus
6.1.1.21
153000
-
gel filtration, cytoplasmic
Bos taurus
6.1.1.21
154000
-
gel filtration, mitochondrial
Bos taurus
6.1.1.22
additional information
-
existence of high molecular weight aminoacyl-tRNA synthetase complexes within the mitochondria and the cytoplasm
Bos taurus
6.1.1.22
175000
-
cytoplasmic enzyme, dissociated activity arises from high molecular weight aminoacyl-tRNA synthetase complex, gel filtration
Bos taurus
6.1.1.22
189000
-
mitochondrial enzyme, dissociated activity arises from high molecular weight aminoacyl-tRNA synthetase complex, gel filtration
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.7
ATP + alanine + tRNAAla
Bos taurus
key role in protein biosynthesis
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.7
partial, from a multienzyme complex containing at least 16 aminoacyl-tRNA synthetase activities
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
6.1.1.3
liver
-
Bos taurus
-
6.1.1.7
liver
-
Bos taurus
-
6.1.1.11
liver
-
Bos taurus
-
6.1.1.12
liver
-
Bos taurus
-
6.1.1.14
liver
-
Bos taurus
-
6.1.1.16
liver
-
Bos taurus
-
6.1.1.17
liver
-
Bos taurus
-
6.1.1.18
liver
-
Bos taurus
-
6.1.1.21
liver
-
Bos taurus
-
6.1.1.22
liver
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.3
ATP + L-threonine + tRNAThr
-
92
Bos taurus
AMP + diphosphate + L-threonyl-tRNAThr
-
92
Bos taurus
-
6.1.1.7
ATP + alanine + tRNAAla
key role in protein biosynthesis
92
Bos taurus
?
-
-
-
-
6.1.1.7
ATP + L-alanine + tRNAAla
-
92
Bos taurus
AMP + diphosphate + L-alanyl-tRNAAla
-
92
Bos taurus
-
6.1.1.11
ATP + L-serine + tRNASer
-
92
Bos taurus
AMP + diphosphate + L-seryl-tRNASer
-
92
Bos taurus
-
6.1.1.12
ATP + L-aspartate + tRNAAsp
-
92
Bos taurus
AMP + diphosphate + L-aspartyl-tRNAAsp
-
92
Bos taurus
-
6.1.1.14
ATP + glycine + tRNAGly
-
92
Bos taurus
AMP + diphosphate + glycyl-tRNAGly
-
92
Bos taurus
-
6.1.1.15
ATP + L-proline + tRNAPro
-
92
Bos taurus
AMP + diphosphate + L-prolyl-tRNAPro
-
92
Bos taurus
-
6.1.1.16
ATP + L-cysteine + tRNACys
-
92
Bos taurus
AMP + diphosphate + L-cysteinyl-tRNACys
-
92
Bos taurus
-
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
92
Bos taurus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
92
Bos taurus
-
6.1.1.18
ATP + L-glutamine + tRNAGln
-
92
Bos taurus
AMP + diphosphate + L-glutaminyl-tRNAGln
-
92
Bos taurus
-
6.1.1.21
ATP + L-histidine + tRNAHis
-
92
Bos taurus
AMP + diphosphate + L-histidyl-tRNAHis
-
92
Bos taurus
-
6.1.1.22
ATP + L-asparagine + tRNAAsn
-
92
Bos taurus
AMP + diphosphate + L-asparaginyl-tRNAAsn
-
92
Bos taurus
-