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Literature summary extracted from

  • Samuelsson, T.; Lundvik, L.
    Purification and some properties of asparagine, lysine, serine, and valine:tRNA ligases from Bacillus stearothermophilus (1978), J. Biol. Chem., 253, 7033-7039.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.6
-
 Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.11 additional information
-
 additional information a temperature-dependent inhibition by conformational change of the enzyme (50°C-70°C) lowers both the catalytic activity and the substrate affinity of the ATP and amino acid sites as indicated by a sharp rise in Km with temperature Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.6 58000
-
 2 * 58000, SDS-PAGE Geobacillus stearothermophilus
6.1.1.6 109000
-
 gel filtration Geobacillus stearothermophilus
6.1.1.6 112000
-
 equilibrium sedimentation measurement Geobacillus stearothermophilus
6.1.1.11 49000
-
 2 * 49000, SDS-PAGE Geobacillus stearothermophilus
6.1.1.11 81000
-
 gel filtration, sedimentation velocity Geobacillus stearothermophilus
6.1.1.11 88000
-
 sedimentation equilibrium measurement Geobacillus stearothermophilus
6.1.1.22 51000
-
 2 * 51000, sedimentation equilibrium measurement under denaturing conditions Geobacillus stearothermophilus
6.1.1.22 120000
-
 calculation from gel filtration and sedimentation velocity Geobacillus stearothermophilus
6.1.1.22 127000
-
 sedimentation equilibrium measurement Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.6 Geobacillus stearothermophilus
-
-
-
6.1.1.11 Geobacillus stearothermophilus
-
-
-
6.1.1.22 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.6
-
 Geobacillus stearothermophilus
6.1.1.11
-
 Geobacillus stearothermophilus
6.1.1.22
-
 Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.6 0.345
-
-
 Geobacillus stearothermophilus
6.1.1.11 0.106
-
-
 Geobacillus stearothermophilus
6.1.1.22 0.113
-
-
 Geobacillus stearothermophilus

Storage Stability

EC Number Storage Stability Organism
6.1.1.6 -80°C, 0.1 M potassium phosphate buffer, pH 7.0 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.6 ATP + lysine + tRNALys
-
 Geobacillus stearothermophilus AMP + L-lysyl-tRNALys + diphosphate
-
 ?
6.1.1.11 ATP + L-serine + tRNASer
-
 Geobacillus stearothermophilus AMP + diphosphate + L-seryl-tRNASer
-
 ?
6.1.1.22 ATP + L-asparagine + tRNAAsn
-
 Geobacillus stearothermophilus AMP + diphosphate + L-asparaginyl-tRNAAsn
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.6 dimer 2 * 58000, SDS-PAGE Geobacillus stearothermophilus
6.1.1.11 dimer 2 * 49000, SDS-PAGE Geobacillus stearothermophilus
6.1.1.22 dimer 2 * 51000, sedimentation equilibrium measurement under denaturing conditions Geobacillus stearothermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.1.1.22 additional information
-
 temperature-induced conformation change of the enzymes affecting primarily the binding sites for ATP and amino acid Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.6 60
-
 after 4 h complete loss of aminoacylation activity, 3 min, stable in presence of all substrates except lysine Geobacillus stearothermophilus
6.1.1.6 70
-
 0.5 min, stable in presence of all substrates except lysine Geobacillus stearothermophilus
6.1.1.22 60
-
 3 min, stable, in presence of all substrates except amino acid Geobacillus stearothermophilus
6.1.1.22 70
-
 0.5 min, stable, in presence of all substrates except amino acid Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.1.1.11 6 8 25°C, acylation rate increases when pH is raised from 6.0 to 8.0 Geobacillus stearothermophilus
6.1.1.22 6 8 25 C, the aminoacylation rate increases as the pH is raised from 6.0 to 8.0 Geobacillus stearothermophilus