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Literature summary extracted from

  • Hersh, L.B.
    Malate adenosine triphosphate lyase. Separation of the reaction into a malate thiokinase and malyl coenzyme A lyase (1973), J. Biol. Chem., 248, 7295-7303.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.2.1.9 rapidly denatured when frozen and thawed one time Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.9 DTNB
-
 Pseudomonas sp.
6.2.1.9 NEM
-
 Pseudomonas sp.
6.2.1.9 p-hydroxymercuribenzoate
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.24 0.035
-
 acetyl-CoA
-
 Pseudomonas sp.
4.1.3.24 0.1
-
 (3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
 Pseudomonas sp.
4.1.3.24 0.8
-
 glyoxylate
-
 Pseudomonas sp.
6.2.1.9 additional information
-
 additional information
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.9 290000
-
 gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.24 Pseudomonas sp.
-
 Shaw strain MA
-
6.2.1.9 Pseudomonas sp.
-
-
-
6.2.1.9 Pseudomonas sp. MA
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.24
-
 Pseudomonas sp.
6.2.1.9
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.24 0.47
-
-
 Pseudomonas sp.
6.2.1.9 4.5
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
6.2.1.9 4°C, stable for several weeks Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.24 (3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
 Pseudomonas sp. acetyl-CoA + glyoxylate
-
 r
4.1.3.24 (3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
 Pseudomonas sp. MA acetyl-CoA + glyoxylate
-
 r
4.1.3.24 acetyl-CoA + glyoxylate
-
 Pseudomonas sp. (3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
 r
6.2.1.9 ATP + 2-mercaptosuccinate + CoA activity is 18% relative to L-malate Pseudomonas sp. ADP + phosphate + 2-mercaptosuccinyl-CoA
-
 ?
6.2.1.9 ATP + 2-mercaptosuccinate + CoA activity is 18% relative to L-malate Pseudomonas sp. MA ADP + phosphate + 2-mercaptosuccinyl-CoA
-
 ?
6.2.1.9 ATP + DL-isocitrate + CoA activity is 27% of the activity relative to L-malate Pseudomonas sp. ADP + phosphate + isocitryl-CoA
-
 ?
6.2.1.9 ATP + DL-isocitrate + CoA activity is 27% of the activity relative to L-malate Pseudomonas sp. MA ADP + phosphate + isocitryl-CoA
-
 ?
6.2.1.9 ATP + malate + CoA activity with D-malate is 28% relative to L-malate Pseudomonas sp. ADP + phosphate + malyl-CoA
-
 ?
6.2.1.9 ATP + malate + CoA activity with D-malate is 28% relative to L-malate Pseudomonas sp. MA ADP + phosphate + malyl-CoA
-
 ?
6.2.1.9 ATP + succinate + CoA activity is 105% relative to L-malate Pseudomonas sp. ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.9 ATP + succinate + CoA activity is 105% relative to L-malate Pseudomonas sp. MA ADP + phosphate + succinyl-CoA
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.2.1.9 37
-
 70% loss of activity after 10 min Pseudomonas sp.