BRENDA - Enzyme Database

Malate adenosine triphosphate lyase. Separation of the reaction into a malate thiokinase and malyl coenzyme A lyase

Hersh, L.B.; J. Biol. Chem. 248, 7295-7303 (1973)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
6.2.1.9
rapidly denatured when frozen and thawed one time
Pseudomonas sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.2.1.9
DTNB
-
Pseudomonas sp.
6.2.1.9
NEM
-
Pseudomonas sp.
6.2.1.9
p-hydroxymercuribenzoate
-
Pseudomonas sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.24
0.035
-
acetyl-CoA
-
Pseudomonas sp.
4.1.3.24
0.1
-
(3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
Pseudomonas sp.
4.1.3.24
0.8
-
glyoxylate
-
Pseudomonas sp.
6.2.1.9
additional information
-
additional information
-
Pseudomonas sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.2.1.9
290000
-
gel filtration
Pseudomonas sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.24
Pseudomonas sp.
-
Shaw strain MA
-
6.2.1.9
Pseudomonas sp.
-
-
-
6.2.1.9
Pseudomonas sp. MA
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.24
-
Pseudomonas sp.
6.2.1.9
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.24
0.47
-
-
Pseudomonas sp.
6.2.1.9
4.5
-
-
Pseudomonas sp.
Storage Stability
EC Number
Storage Stability
Organism
6.2.1.9
4°C, stable for several weeks
Pseudomonas sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.24
(3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
856
Pseudomonas sp.
acetyl-CoA + glyoxylate
-
856
Pseudomonas sp.
r
4.1.3.24
(3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
856
Pseudomonas sp. MA
acetyl-CoA + glyoxylate
-
856
Pseudomonas sp. MA
r
4.1.3.24
acetyl-CoA + glyoxylate
-
856
Pseudomonas sp.
(3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
856
Pseudomonas sp.
r
6.2.1.9
ATP + 2-mercaptosuccinate + CoA
activity is 18% relative to L-malate
856
Pseudomonas sp.
ADP + phosphate + 2-mercaptosuccinyl-CoA
-
-
-
-
6.2.1.9
ATP + 2-mercaptosuccinate + CoA
activity is 18% relative to L-malate
856
Pseudomonas sp. MA
ADP + phosphate + 2-mercaptosuccinyl-CoA
-
-
-
-
6.2.1.9
ATP + DL-isocitrate + CoA
activity is 27% of the activity relative to L-malate
856
Pseudomonas sp.
ADP + phosphate + isocitryl-CoA
-
-
-
-
6.2.1.9
ATP + DL-isocitrate + CoA
activity is 27% of the activity relative to L-malate
856
Pseudomonas sp. MA
ADP + phosphate + isocitryl-CoA
-
-
-
-
6.2.1.9
ATP + malate + CoA
activity with D-malate is 28% relative to L-malate
856
Pseudomonas sp.
ADP + phosphate + malyl-CoA
-
-
-
-
6.2.1.9
ATP + malate + CoA
activity with D-malate is 28% relative to L-malate
856
Pseudomonas sp. MA
ADP + phosphate + malyl-CoA
-
-
-
-
6.2.1.9
ATP + succinate + CoA
activity is 105% relative to L-malate
856
Pseudomonas sp.
ADP + phosphate + succinyl-CoA
-
-
-
-
6.2.1.9
ATP + succinate + CoA
activity is 105% relative to L-malate
856
Pseudomonas sp. MA
ADP + phosphate + succinyl-CoA
-
-
-
-
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.2.1.9
37
-
70% loss of activity after 10 min
Pseudomonas sp.
General Stability (protein specific)
EC Number
General Stability
Organism
6.2.1.9
rapidly denatured when frozen and thawed one time
Pseudomonas sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.2.1.9
DTNB
-
Pseudomonas sp.
6.2.1.9
NEM
-
Pseudomonas sp.
6.2.1.9
p-hydroxymercuribenzoate
-
Pseudomonas sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.24
0.035
-
acetyl-CoA
-
Pseudomonas sp.
4.1.3.24
0.1
-
(3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
Pseudomonas sp.
4.1.3.24
0.8
-
glyoxylate
-
Pseudomonas sp.
6.2.1.9
additional information
-
additional information
-
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.2.1.9
290000
-
gel filtration
Pseudomonas sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.24
-
Pseudomonas sp.
6.2.1.9
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.24
0.47
-
-
Pseudomonas sp.
6.2.1.9
4.5
-
-
Pseudomonas sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
6.2.1.9
4°C, stable for several weeks
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.24
(3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
856
Pseudomonas sp.
acetyl-CoA + glyoxylate
-
856
Pseudomonas sp.
r
4.1.3.24
(3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
856
Pseudomonas sp. MA
acetyl-CoA + glyoxylate
-
856
Pseudomonas sp. MA
r
4.1.3.24
acetyl-CoA + glyoxylate
-
856
Pseudomonas sp.
(3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
856
Pseudomonas sp.
r
6.2.1.9
ATP + 2-mercaptosuccinate + CoA
activity is 18% relative to L-malate
856
Pseudomonas sp.
ADP + phosphate + 2-mercaptosuccinyl-CoA
-
-
-
-
6.2.1.9
ATP + 2-mercaptosuccinate + CoA
activity is 18% relative to L-malate
856
Pseudomonas sp. MA
ADP + phosphate + 2-mercaptosuccinyl-CoA
-
-
-
-
6.2.1.9
ATP + DL-isocitrate + CoA
activity is 27% of the activity relative to L-malate
856
Pseudomonas sp.
ADP + phosphate + isocitryl-CoA
-
-
-
-
6.2.1.9
ATP + DL-isocitrate + CoA
activity is 27% of the activity relative to L-malate
856
Pseudomonas sp. MA
ADP + phosphate + isocitryl-CoA
-
-
-
-
6.2.1.9
ATP + malate + CoA
activity with D-malate is 28% relative to L-malate
856
Pseudomonas sp.
ADP + phosphate + malyl-CoA
-
-
-
-
6.2.1.9
ATP + malate + CoA
activity with D-malate is 28% relative to L-malate
856
Pseudomonas sp. MA
ADP + phosphate + malyl-CoA
-
-
-
-
6.2.1.9
ATP + succinate + CoA
activity is 105% relative to L-malate
856
Pseudomonas sp.
ADP + phosphate + succinyl-CoA
-
-
-
-
6.2.1.9
ATP + succinate + CoA
activity is 105% relative to L-malate
856
Pseudomonas sp. MA
ADP + phosphate + succinyl-CoA
-
-
-
-
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.2.1.9
37
-
70% loss of activity after 10 min
Pseudomonas sp.