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Literature summary extracted from

  • Jakubowski, H.; Pawelkiewicz, J.
    The plant aminoacyl-tRNA synthetases. Purification and characterization of valyl-tRNA, tryptophanyl-tRNA and seryl-tRNA synthetases from yellow lupin seeds (1975), Eur. J. Biochem., 52, 301-310.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.11 Intact SH-groups required for activity Lupinus luteus

General Stability

EC Number General Stability Organism
6.1.1.2 requires intact -SH groups for their activity Lupinus luteus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.2 dATP
-
Lupinus luteus
6.1.1.2 NEM
-
Lupinus luteus
6.1.1.2 p-hydroxymercuribenzoate
-
Lupinus luteus
6.1.1.11 2-mercaptoethanol
-
Lupinus luteus
6.1.1.11 p-hydroxymercuribenzoate
-
Lupinus luteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.2 0.0002
-
tRNATrp
-
Lupinus luteus
6.1.1.2 0.005
-
Trp
-
Lupinus luteus
6.1.1.2 0.03
-
ATP
-
Lupinus luteus
6.1.1.11 0.0005
-
tRNASer
-
Lupinus luteus
6.1.1.11 0.012
-
Ser
-
Lupinus luteus
6.1.1.11 0.04
-
ATP
-
Lupinus luteus
6.1.1.11 0.065
-
dATP
-
Lupinus luteus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.2 KCl optimal concentration: 50 mM Lupinus luteus
6.1.1.2 Mg2+ Km: 1.5 mM Lupinus luteus
6.1.1.11 KCl 50 mM, required for optimal activity Lupinus luteus
6.1.1.11 Mg2+ required Lupinus luteus
6.1.1.11 Mg2+ Km: , 3.5 mM Lupinus luteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.2 37000
-
possibly 4 * 37000, SDS-PAGE Lupinus luteus
6.1.1.2 200000
-
gel filtration Lupinus luteus
6.1.1.11 55000
-
2 * 55000, SDS-PAGE Lupinus luteus
6.1.1.11 110000
-
gel filtration Lupinus luteus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.2 Lupinus luteus
-
-
-
6.1.1.11 Lupinus luteus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.2
-
Lupinus luteus
6.1.1.11
-
Lupinus luteus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.2 seed
-
Lupinus luteus
-
6.1.1.11 seed
-
Lupinus luteus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.2 0.45
-
-
Lupinus luteus
6.1.1.11 0.15
-
-
Lupinus luteus

Storage Stability

EC Number Storage Stability Organism
6.1.1.2 -20°C, 50% v/v glycerol, 5 mM potassium phosphate buffer, pH 6.8, 1 mM 2-mercaptoethanol Lupinus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.2 ATP + L-tryptophan + tRNATrp
-
Lupinus luteus AMP + diphosphate + L-tryptophan-tRNATrp
-
?
6.1.1.2 additional information not: dATP Lupinus luteus ?
-
?
6.1.1.11 ATP + L-serine + tRNASer
-
Lupinus luteus AMP + diphosphate + L-seryl-tRNASer
-
?
6.1.1.11 dATP + L-serine + tRNASer
-
Lupinus luteus dAMP + diphosphate + L-seryl-tRNASer
-
?

Subunits

EC Number Subunits Comment Organism
6.1.1.2 tetramer possibly 4 * 37000, SDS-PAGE Lupinus luteus
6.1.1.11 dimer 2 * 55000, SDS-PAGE Lupinus luteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.2 6.5 7.5 in cacodylate buffer Lupinus luteus
6.1.1.2 8
-
in phosphate buffer Lupinus luteus
6.1.1.2 8.5
-
in Hepes buffer Lupinus luteus
6.1.1.2 8.5 9.2 in Tris buffer Lupinus luteus
6.1.1.11 7.1 8.1 in phosphate and Tris buffer Lupinus luteus
6.1.1.11 8.1
-
Hepes buffer Lupinus luteus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.1.1.11 7.3 10 7.3: about 30% of maximal activity, 10: about 60% of activity maximum Lupinus luteus