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Literature summary extracted from

  • Luo, G.X.; Nishimura, J.S.
    Adenosine 5ฆ-tetraphosphate is synthesized by the histidine alpha142-->asparagine mutant of Escherichia coli succinyl-CoA synthetase (1992), J. Biol. Chem., 267, 9516-9520.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.5 N142N mutant H142N is devoid of the ability to catalyze the overall reaction but is able to catalyze the half-reactions at significant rates. Phosphorylation by ATP and dephosphorylation by ADP of the mutant enzyme occurs at rates that are at least 10times greater than those with wild type enzyme. Dephosphorylation by succinate plus CoA, succinyl-CoA formation, proceeds with a maximal velocity of 10% that of wild type enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.5 ADP adenosine 5ยด-tetraphosphate formation Escherichia coli
6.2.1.5 CoA plus succinate presence of succinate plus CoA inhibits adenosine 5'-tetraphosphate formation Escherichia coli
6.2.1.5 succinate presence of succinate plus CoA inhibits adenosine 5'-tetraphosphate formation Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.5 0.0145
-
 ATP adenosine 5ยด-tetraphosphate formation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.5 Escherichia coli
-
 wild type and mutant H142N
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.5 ATP + ATP
-
 Escherichia coli adenosine 5'-tetraphosphate + ADP
-
 ?
6.2.1.5 ATP + succinate + CoA
-
 Escherichia coli ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 beta,gamma-methylene-adenosine 5'-triphosphate
-
 Escherichia coli beta,gamma-methylene-adenosine 5'-tetraphosphate + ? alpha,gamma-methylene adenosine tetraphosphate ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.5 additional information
-
 additional information
-
 Escherichia coli