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Literature summary extracted from

  • Orlowski, M.; Wilk, S.
    Purification and specificity of a membrane-bound metalloendopeptidase from bovine pituitaries (1981), Biochemistry, 20, 4942-4950.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.11 1,10-phenanthroline
-
 Bos taurus
3.4.24.11 2-mercaptoethanol
-
 Bos taurus
3.4.24.11 diisopropyl fluorophosphate
-
 Bos taurus
3.4.24.11 dithiothreitol
-
 Bos taurus
3.4.24.11 EDTA
-
 Bos taurus
3.4.24.11 GSH
-
 Bos taurus
3.4.24.11 L-Cys
-
 Bos taurus
3.4.24.11 pepstatin
-
 Bos taurus
3.4.24.11 phosphate
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.11 0.03
-
 Nalpha-benzoyl-Gly-Lys-Lys-Arg-Arg-Leu-2-naphthylamide
-
 Bos taurus
3.4.24.11 0.08
-
 Nalpha-benzoyl-Gly-Lys-Arg-Arg-Leu-2-naphthylamide
-
 Bos taurus
3.4.24.11 0.11
-
 Nalpha-benzoyl-Gly-Arg-Arg-Phe-2-naphthylamide
-
 Bos taurus
3.4.24.11 0.11
-
 Nalpha-benzoyl-Gly-Gly-Arg-Leu-2-naphthylamide
-
 Bos taurus
3.4.24.11 0.13
-
 N-benzyoxycarbonyl-Gly-Gly-Leu 2-naphthylamide
-
 Bos taurus
3.4.24.11 0.18
-
 Nalpha-benzoyl-Gly-Arg-Arg-Leu-2-naphthylamide
-
 Bos taurus
3.4.24.11 0.19
-
 Nalpha-benzoyl-Gly-Arg-Leu-2-naphthylamide
-
 Bos taurus
3.4.24.11 0.3
-
 Nalpha-benzoyl-Gly-Arg-Arg-Ala-2-naphthylamide
-
 Bos taurus
3.4.24.11 0.42
-
 N-(4-carboxybutanoyl)-L-alanyl-L-alanyl-N-(4-methoxynaphthalen-2-yl)-L-phenylalaninamide
-
 Bos taurus
3.4.24.11 0.45
-
 succinyl-Arg-Arg-Leu-2-naphthylamide
-
 Bos taurus
3.4.24.11 0.59
-
 glutaryl-Ala-Ala-Phe-2-naphthylamide
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.11 membrane bound Bos taurus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.11 90000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.11 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.11
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.11 hypophysis
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.11 benzoyl-Gly-Gly-Arg-Leu-2-naphthylamide + H2O
-
 Bos taurus benzoyl-Gly-Gly-Arg + L-Leu-2-naphthylamide
-
 ?
3.4.24.11 glutaryl-Ala-Ala-Phe-2-naphthylamide + H2O
-
 Bos taurus glutaryl-Ala-Ala + Phe-2-naphthylamide
-
 ?
3.4.24.11 glutaryl-Ala-Ala-Phe-4-methoxy-2-naphthylamide + H2O
-
 Bos taurus glutaryl-Ala-Ala + Phe-4-methoxy-2-naphthylamide
-
 ?
3.4.24.11 hippuryl-Arg-Arg-Leu-2-naphthylamide + H2O
-
 Bos taurus hippuryl-Arg-Arg + Leu-2-naphthylamide
-
 ?
3.4.24.11 N-benzyloxycarbonyl-Gly-Gly-Leu 2-naphthylamide + H2O
-
 Bos taurus N-benzyloxycarbonyl-Gly-Gly + L-leucine 2-naphthylamide
-
 ?
3.4.24.11 N-benzyoxycarbonyl-Gly-Gly-Leu 2-naphthylamide + H2O
-
 Bos taurus ?
-
 ?
3.4.24.11 N-benzyoxycarbonyl-Gly-Gly-Leu-2-naphthylamide + H2O
-
 Bos taurus N-benzyoxycarbonyl-Gly-Gly + L-Leu-2-naphthylamide
-
 ?
3.4.24.11 Nalpha-benzoyl-Gly-Arg-Arg-Ala-2-naphthylamide + H2O
-
 Bos taurus Nalpha-benzoyl-Gly-Arg-Arg + Ala-2-naphthylamide
-
 ?
3.4.24.11 Nalpha-benzoyl-Gly-Arg-Arg-Leu-2-naphthylamide + H2O
-
 Bos taurus Nalpha-benzoyl-Gly-Arg-Arg + Leu-2-naphthylamide
-
 ?
3.4.24.11 Nalpha-benzoyl-Gly-Arg-Arg-Phe-2-naphthylamide + H2O
-
 Bos taurus Nalpha-benzoyl-Gly-Arg-Arg + Phe-2-naphthylamide
-
 ?
3.4.24.11 Nalpha-benzoyl-Gly-Arg-Leu-2-naphthylamide + H2O
-
 Bos taurus Nalpha-benzoyl-Gly-Arg + Leu-2-naphthylamide
-
 ?
3.4.24.11 Nalpha-benzoyl-Gly-Gly-Arg-Leu-2-naphthylamide + H2O
-
 Bos taurus Nalpha-benzoyl-Gly-Gly-Arg + Leu-2-naphthylamide
-
 ?
3.4.24.11 Nalpha-benzoyl-Gly-Lys-Arg-Arg-Leu-2-naphthylamide + H2O
-
 Bos taurus Nalpha-benzoyl-Gly-Lys-Arg-Arg + Leu-2-naphthylamide
-
 ?
3.4.24.11 Nalpha-benzoyl-Gly-Lys-Lys-Arg-Arg-Leu-2-naphthylamide + H2O
-
 Bos taurus Nalpha-benzoyl-Gly-Lys-Lys-Arg-Arg + Leu-2-naphthylamide
-
 ?
3.4.24.11 succinyl-Arg-Arg-Leu-2-naphthylamide + H2O
-
 Bos taurus succinyl-Arg-Arg + Leu-2-naphthylamide
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.11 0.0483
-
 Nalpha-benzoyl-Gly-Arg-Leu-2-naphthylamide
-
 Bos taurus
3.4.24.11 0.05
-
 benzoyl-Gly-Gly-Arg-Leu-2-naphthylamide
-
 Bos taurus
3.4.24.11 0.105
-
 N-benzyoxycarbonyl-Gly-Gly-Leu-2-naphthylamide
-
 Bos taurus
3.4.24.11 0.147
-
 Nalpha-benzoyl-Gly-Arg-Arg-Ala-2-naphthylamide
-
 Bos taurus
3.4.24.11 0.383
-
 Nalpha-benzoyl-Gly-Lys-Lys-Arg-Arg-Leu-2-naphthylamide
-
 Bos taurus
3.4.24.11 1.82
-
 Nalpha-benzoyl-Gly-Lys-Arg-Arg-Leu-2-naphthylamide
-
 Bos taurus
3.4.24.11 2
-
 Nalpha-benzoyl-Gly-Arg-Arg-Phe-2-naphthylamide
-
 Bos taurus
3.4.24.11 4
-
 Nalpha-benzoyl-Gly-Arg-Arg-Leu-2-naphthylamide
-
 Bos taurus
3.4.24.11 5.28
-
 glutaryl-Ala-Ala-Phe-4-methoxy-2-naphthylamide
-
 Bos taurus
3.4.24.11 6.32
-
 succinyl-Arg-Arg-Leu-2-naphthylamide
-
 Bos taurus
3.4.24.11 9.87
-
 glutaryl-Ala-Ala-Phe-2-naphthylamide
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.11 7.3 7.8 hydrolysis of hippuryl-Arg-Arg-Leu-2-naphthylamide Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.11 7 8.4 about 80% of maximal activity at pH 7.0 and at pH 8.4, hydrolysis of hippuryl-Arg-Arg-Leu-2-naphthylamide Bos taurus