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Literature summary extracted from
- Characterisation of neprilysin (EC 3.4.24.11) S2 subsite (1997), FEBS Lett., 411, 140-144.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.24.11 | N542G | about 12fold increased Km-value for Leu5,Arg6-enkephalin | Oryctolagus cuniculus |
| 3.4.24.11 | R102M | about 2fold increased Km-value for Leu5,Arg6-enkephalin, no inhibition by Phe-Gly | Oryctolagus cuniculus |
| 3.4.24.11 | R102M/N542G | about 20fold increased Km-value for Leu5,Arg6-enkephalin | Oryctolagus cuniculus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.11 | Phe-Gly | - |
Oryctolagus cuniculus |  |
| 3.4.24.11 | Phe-Trp | - |
Oryctolagus cuniculus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.24.11 | 0.109 | - |
Ala-Leu-enkephalin | wild type enzyme | Oryctolagus cuniculus | |
| 3.4.24.11 | 0.24 | - |
D-Ala2-Leu5-enkephalin | mutant enzyme R102M/N542G | Oryctolagus cuniculus | |
| 3.4.24.11 | 1.399 | - |
D-Ala2-Leu5-enkephalin | mutant enzyme N542G | Oryctolagus cuniculus | |
| 3.4.24.11 | 2.137 | - |
D-Ala2-Leu5-enkephalin | mutant enzyme R102M/N542G | Oryctolagus cuniculus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.11 | Oryctolagus cuniculus | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.11 | Ala-Leu-enkephalin + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
| 3.4.24.11 | D-Ala2-Leu5-enkephalin + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.24.11 | 232 | - |
D-Ala2-Leu5-enkephalin | mutant enzyme N542G | Oryctolagus cuniculus | |
| 3.4.24.11 | 412 | - |
D-Ala2-Leu5-enkephalin | mutant enzyme R102M/N542G | Oryctolagus cuniculus | |
| 3.4.24.11 | 1020 | - |
D-Ala2-Leu5-enkephalin | wild type enzyme | Oryctolagus cuniculus | |
| 3.4.24.11 | 1260 | - |
D-Ala2-Leu5-enkephalin | mutant enzyme R102M | Oryctolagus cuniculus | |
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