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Literature summary extracted from

  • Mitchell, W.M.; Harrington, W.F.
    Clostripain (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 3, 699-719.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.8 Borate partial inhibition Hathewaya histolytica
3.4.22.8 butylguanidine most effective competitive inhibitor, hydrolysis of benzoyl-Arg ethyl ester Hathewaya histolytica
3.4.22.8 Cd2+
-
 Hathewaya histolytica
3.4.22.8 citrate partial inhibition Hathewaya histolytica
3.4.22.8 Co2+
-
 Hathewaya histolytica
3.4.22.8 Cu2+
-
 Hathewaya histolytica
3.4.22.8 EDTA
-
 Hathewaya histolytica
3.4.22.8 H2O2 complete regeneration by reduction with dithiothreitol Hathewaya histolytica
3.4.22.8 K+
-
 Hathewaya histolytica
3.4.22.8 Na+
-
 Hathewaya histolytica
3.4.22.8 PCMB
-
 Hathewaya histolytica
3.4.22.8 Polyarginine hydrolysis of benzoyl-Arg ethyl ester Hathewaya histolytica
3.4.22.8 polylysine hydrolysis of benzoyl-Arg ethyl ester Hathewaya histolytica
3.4.22.8 RCM RNase hydrolysis of benzoyl-Arg ethyl ester Hathewaya histolytica
3.4.22.8 Tris partial inhibition Hathewaya histolytica
3.4.22.8 Veronal partial inhibition Hathewaya histolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.8 0.022
-
 p-toluenesulfonyl-L-Arg methyl ester
-
 Hathewaya histolytica
3.4.22.8 0.22
-
 benzoyl-Arg ethyl ester
-
 Hathewaya histolytica
3.4.22.8 0.25
-
 Thr-Ala-Ala
-
 Hathewaya histolytica
3.4.22.8 1.3
-
 N-benzoyl-L-Arg amide
-
 Hathewaya histolytica
3.4.22.8 2.5
-
 p-Nitrophenyl p-guanidinobenzoate
-
 Hathewaya histolytica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.8 Ca2+ enhances esterase activity Hathewaya histolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.8 50000
-
-
 Hathewaya histolytica

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.8 Hathewaya histolytica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.8
-
 Hathewaya histolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.8 L-Arg methyl ester + H2O
-
 Hathewaya histolytica L-Arg + methanol
-
 ?
3.4.22.8 additional information the major activity is directed towards the carboxyl peptide linkage of Arg Hathewaya histolytica ?
-
 ?
3.4.22.8 N-benzoyl-Arg amide + H2O
-
 Hathewaya histolytica N-benzoyl-Arg + NH3
-
 ?
3.4.22.8 N-benzoyl-Arg benzyl ester + H2O
-
 Hathewaya histolytica N-benzoyl-Arg + benzyl alcohol
-
 ?
3.4.22.8 N-benzoyl-Arg ethyl ester + H2O
-
 Hathewaya histolytica N-benzoyl-Arg + ethanol
-
 ?
3.4.22.8 N-benzoyl-Arg isopropyl ester + H2O
-
 Hathewaya histolytica N-benzoyl-Arg isopropanol
-
 ?
3.4.22.8 N-benzoyl-Arg methyl ester + H2O
-
 Hathewaya histolytica N-benzoyl-Arg + methanol
-
 ?
3.4.22.8 Nalpha-benzoyl-L-Arg 2-naphthylamide + H2O
-
 Hathewaya histolytica Nalpha-benzoyl-L-Arg + 2-naphthylamine
-
 ?
3.4.22.8 p-nitrophenyl p-guanidinobenzoate + H2O
-
 Hathewaya histolytica p-nitrophenol + p-guanidinobenzoate
-
 ?
3.4.22.8 p-tosyl-Arg methyl ester + H2O
-
 Hathewaya histolytica p-tosyl-Arg + methanol
-
 ?
3.4.22.8 Thr-Ala-Ala + H2O
-
 Hathewaya histolytica ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.8 additional information
-
 Ca2+ depresses thermal inactivation Hathewaya histolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.8 7.2
-
 hydrolysis of L-Arg methyl ester, in phosphate buffer Hathewaya histolytica
3.4.22.8 7.4 7.8 hydrolysis of alpha-benzoyl-Arg ethyl ester Hathewaya histolytica
3.4.22.8 7.4 7.8 in phosphate or Tris-HCl buffer Hathewaya histolytica