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Literature summary extracted from

  • Tablero, M.; Arreguin, R.; Arreguin, B.; Soriano, M.; Sanchez, R.I.; Romero, A.R.; Hernandez-Arana, A.
    Purification and characterization of multiple forms of asclepain g from Asclepias glaucescens H.B.K. (1991), Plant Sci., 74, 7-15.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.7 21800
-
 asclepain Ag3, gel filtration Asclepias glaucescens
3.4.22.7 22500
-
 asclepain Ag6, gel filtration Asclepias glaucescens
3.4.22.7 22600
-
 asclepain Ag7, gel filtration Asclepias glaucescens
3.4.22.7 22700
-
 asclepain Ag8, gel filtration Asclepias glaucescens
3.4.22.7 23300
-
 1 * 23300, asclepain Ag7, SDS-PAGE Asclepias glaucescens
3.4.22.7 23400
-
 1 * 23400, asclepain Ag3, SDS-PAGE Asclepias glaucescens
3.4.22.7 24200
-
 1 * 24200, asclepain Ag8, SDS-PAGE Asclepias glaucescens
3.4.22.7 24400
-
 1 * 24400, asclepain Ag6, SDS-PAGE Asclepias glaucescens

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.7 Asclepias glaucescens
-
 10 asclepain forms
-
3.4.22.7 Asclepias glaucescens H.B.K
-
 10 asclepain forms
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.7
-
 Asclepias glaucescens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.7 latex
-
 Asclepias glaucescens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.7 casein + H2O
-
 Asclepias glaucescens ?
-
 ?
3.4.22.7 casein + H2O
-
 Asclepias glaucescens H.B.K ?
-
 ?
3.4.22.7 N-acetyl-L-tryptophanamide + H2O
-
 Asclepias glaucescens N-acetyl-L-Trp + NH3
-
 ?
3.4.22.7 N-acetyl-L-tryptophanamide + H2O
-
 Asclepias glaucescens H.B.K N-acetyl-L-Trp + NH3
-
 ?
3.4.22.7 N-Benzoyl-L-Arg ethyl ester + H2O
-
 Asclepias glaucescens N-Benzoyl-L-Arg + ethanol
-
 ?
3.4.22.7 N-Benzoyl-L-Arg ethyl ester + H2O
-
 Asclepias glaucescens H.B.K N-Benzoyl-L-Arg + ethanol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.7 monomer 1 * 23300, asclepain Ag7, SDS-PAGE Asclepias glaucescens
3.4.22.7 monomer 1 * 24200, asclepain Ag8, SDS-PAGE Asclepias glaucescens
3.4.22.7 monomer 1 * 24400, asclepain Ag6, SDS-PAGE Asclepias glaucescens
3.4.22.7 monomer 1 * 23400, asclepain Ag3, SDS-PAGE Asclepias glaucescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.7 6 8 hydrolysis of N-benzoyl-L-Arg ethyl ester, asclepain Ag7 Asclepias glaucescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.7 5 9 pH 5.0: about 70% of maximal activity, pH 9.0: about 30% of maximal activity, hydrolysis of N-benzoyl-L-Arg ethyl ester, asclepain Ag7 Asclepias glaucescens