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Literature summary extracted from

  • Salih, E.; Malthouse, J.P.G.; Kowlessur, S.; Jarvis, M.; O'Driscoll, M.; Brocklehurst, K.
    Differences in the chemical and catalytic characteristics of two crystallographically identical enzyme catalytic sites. Characterization of actinidin and papain by a combination of pH-dependent substrate catalysis kinetics and reactivity probe studies tar (1987), Biochem. J., 247, 181-193.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.22.14
-
 Actinidia chinensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.14 1.8
-
 Nalpha-benzoyl-L-Arg p-nitroanilide pH 7.1 Actinidia chinensis
3.4.22.14 2.6
-
 Nalpha-benzoyl-L-Arg p-nitroanilide pH 4.2 Actinidia chinensis
3.4.22.14 11.2
-
 Nalpha-benzoyl-L-Arg p-nitroanilide pH 9.5 Actinidia chinensis

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.14 Actinidia chinensis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.14 Nalpha-benzoyl-L-Arg p-nitroanilide + H2O
-
 Actinidia chinensis Nalpha-benzoyl-L-Arg + 4-nitroaniline
-
 ?

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.14 2.1
-
 1-2 s, 40% of the catalytic sites survive Actinidia chinensis
3.4.22.14 2.4
-
 1-2 s, 75% of the catalytic sites survive Actinidia chinensis
3.4.22.14 2.5
-
 above, 1-2 s, stable Actinidia chinensis