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Literature summary extracted from

  • Kortt, A.A.; Liu, T.Y.
    On the mechanism of action of streptococcal proteinase. 3. The effect of pH, organic solvents, and deuterium oxide on the proteinase-catalyzed hydrolysis of N-acylamino acid esters (1973), Biochemistry, 12, 338-345.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.10 1,3-Dibromoacetone rapid and complete loss of activity Streptococcus sp.
3.4.22.10 acetonitrile mixed type Streptococcus sp.
3.4.22.10 dimethyl sulfoxide competitive Streptococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.10 additional information
-
 additional information effect of pH-value on the Km-value for phenyl alpha-N-benzyloxycarbonyl-L-lysinate Streptococcus sp.
3.4.22.10 0.588
-
 Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester at pH 7.25 Streptococcus sp.
3.4.22.10 0.605
-
 Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester at pH 6.73 Streptococcus sp.
3.4.22.10 0.637
-
 Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester at pH 6.99 Streptococcus sp.
3.4.22.10 0.664
-
 Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester at pH 6.21 Streptococcus sp.
3.4.22.10 0.667
-
 Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester at pH 5.53 Streptococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.10 Streptococcus sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.10 Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester + H2O
-
 Streptococcus sp. ?
-
 ?
3.4.22.10 Nalpha-benzyloxycarbonyl-Lys-phenyl ester + H2O
-
 Streptococcus sp. Nalpha-benzyloxycarbonyl-Lys + phenol
-
 ?
3.4.22.10 p-nitrophenyl-benzyloxycarbonyl-L-Ala + H2O
-
 Streptococcus sp. ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.10 additional information
-
 additional information effect of pH-value on the turnover number for phenyl alpha-N-benzyloxycarbonyl-L-lysinate Streptococcus sp.
3.4.22.10 174
-
 Nalpha-benzyloxycarbonyl-Lys-phenyl ester at pH 7.25 Streptococcus sp.
3.4.22.10 176
-
 Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester at pH 5.53 Streptococcus sp.
3.4.22.10 178
-
 Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester at pH 6.73 Streptococcus sp.
3.4.22.10 182
-
 Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester at pH 6.21 Streptococcus sp.
3.4.22.10 190
-
 Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester at pH 6.99 Streptococcus sp.