Any feedback?
Literature summary extracted from
- Human complement serine proteases C1r and C1s and their proenzymes (1993), Methods Enzymol., 223, 61-82.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.21.41 | C1rbar undergoes autolytic cleavage | Homo sapiens |
| 3.4.21.42 | no autolytic cleavage | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.41 | 4-Nitrophenyl-4-guanidinobenzoate | - |
Homo sapiens |  |
| 3.4.21.41 | Ca2+ | reaction with C1s | Homo sapiens | |
| 3.4.21.41 | diisopropyl fluorophosphate | - |
Homo sapiens | |
| 3.4.21.41 | leupeptin | competitive | Homo sapiens | |
| 3.4.21.41 | additional information | high ionic strength inhibits | Homo sapiens | |
| 3.4.21.41 | phenylmethylsulfonyl fluoride | - |
Homo sapiens | |
| 3.4.21.42 | C1bar inhibitor | - |
Homo sapiens | |
| 3.4.21.42 | DFP | - |
Homo sapiens | |
| 3.4.21.42 | p-nitrophenyl-p'-guanidinobenzoate | - |
Homo sapiens | |
| 3.4.21.42 | PMSF | - |
Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.42 | 0.056 | 0.07 | N-benzyloxycarbonyl-Tyr p-nitrophenyl ester | - |
Homo sapiens | |
| 3.4.21.42 | 0.069 | - |
N-benzyloxycarbonyl-Gly-Arg thiobenzyl ester | - |
Homo sapiens | |
| 3.4.21.42 | 1.7 | 2.8 | tosyl-Arg methyl ester | - |
Homo sapiens | |
| 3.4.21.42 | 2.5 | 5 | benzoyl-Arg ethyl ester | - |
Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.41 | additional information | - |
MW of C1r: 166000-188000 Da, gel filtration | Homo sapiens |
| 3.4.21.42 | 78900 | - |
x * 78900, monomer protein that forms Ca2+-dependent dimers, calculation from amino acid sequence and carbohydrate content | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.41 | Homo sapiens | - |
- |
- |
| 3.4.21.42 | Homo sapiens | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.21.41 | proteolytic modification | autolytic proteolysis involves cleavage of the Arg279-Gly280 bond in the sequence Asp-Ser-Arg-Gly-Trp-Lys | Homo sapiens |
| 3.4.21.42 | proteolytic modification | activation occurs through cleavage of a single Arg-Ile bond by C1rbar that yields active proteases comprising two chains linked by a single disulfide bride | Homo sapiens |
| 3.4.21.42 | proteolytic modification | C1s is the proenzyme of C1sbar | Homo sapiens |
| 3.4.21.42 | side-chain modification | glycoprotein | Homo sapiens |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.41 | - |
Homo sapiens |
| 3.4.21.42 | C1s and C1sbar | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.41 | serum | - |
Homo sapiens | - |
| 3.4.21.42 | serum | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.41 | complement component C1s + H2O | cleavage of a single Arg-Ile bond in the sequence Lys-Gln-Arg-Ile-Ile-Gly | Homo sapiens | complement component C1sbar | - |
? | |
| 3.4.21.41 | N-acetyl-Gly-Lys methyl ester + H2O | - |
Homo sapiens | N-acetyl-Gly-Lys + methanol | - |
? | |
| 3.4.21.41 | N-benzyloxycarbonyl-Gly-Arg thiobenzyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.41 | N-carbobenzoxy-Lys-p-nitrophenyl ester + H2O | - |
Homo sapiens | N-carbobenzoxy-Lys + 4-nitrophenyl | - |
? | |
| 3.4.21.42 | Benzoyl-Arg ethyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.42 | N-acetyl-Arg methyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.42 | N-acetyl-Gly-Lys methyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.42 | N-benzyloxycarbonyl-Gly-Arg thiobenzyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.42 | N-benzyloxycarbonyl-Lys-p-nitrophenyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.42 | N-benzyloxycarbonyl-Tyr-p-nitrophenyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.42 | Nalpha-acetyl-Tyr ethyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.42 | Nalpha-tosyl-Arg methyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.42 | Tosyl-Arg methyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.41 | More | C1rbar and the proenzyme C1r are noncovalent dimers, the subunit of C1r has a MW of 53000-85000 Da, SDS-PAGE | Homo sapiens |
| 3.4.21.42 | ? | x * 78900, monomer protein that forms Ca2+-dependent dimers, calculation from amino acid sequence and carbohydrate content | Homo sapiens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.41 | additional information | - |
high temperature transition occurs at 53°C, the low-temperature transition varies between 26°C at low ionic strength and 40°C in the presence of 0.5 M NaCl | Homo sapiens |
| 3.4.21.42 | 49 | - |
10 min, 90% loss of activity | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.41 | 8 | - |
- |
Homo sapiens |
| 3.4.21.42 | 7 | 8 | - |
Homo sapiens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.42 | 5 | 9.5 | irreversible inactivation below pH 5.0 and above pH 9.5 | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
