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Literature summary extracted from

  • Newlands, G.F.Y.; Knox, D.P.; Pirie-Sheperd, S.R.; Miller, H.R.P.
    Biochemical and immunological characterization of multiple glycoforms of mouse mast cell protease 1: comparison with an isolated murine serosal mast cell protease (MMCP-4) (1993), Biochem. J., 294, 127-135.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.39 3,4-dichloroisocoumarin
-
 Mus musculus
3.4.21.39 Alpha1-proteinase inhibitor
-
 Mus musculus
3.4.21.39 diisopropyl fluorophosphate
-
 Mus musculus
3.4.21.39 phenylmethylsulfonyl fluoride
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.39 0.663
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide enzyme form 1C Mus musculus
3.4.21.39 0.832
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide enzyme form 1E Mus musculus
3.4.21.39 0.922
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide enzyme form 1A Mus musculus
3.4.21.39 0.936
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide enzyme form 1D Mus musculus
3.4.21.39 0.991
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide enzyme form 1B Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.39 30000
-
 1 * 30000, enzyme form MMCP-1D and MMCP-1E, SDS-PAGE Mus musculus
3.4.21.39 31000
-
 1 * 31000, enzyme form MMCP-1B and MMCP-1C, SDS-PAGE Mus musculus
3.4.21.39 32000
-
 1 * 32000 + 1 * 33000, enzyme form MMCP-1A, SDS-PAGE Mus musculus
3.4.21.39 33000
-
 1 * 32000 + 1 * 33000, enzyme form MMCP-1A, SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.39 Mus musculus
-
 5 enzyme forms: MMCP-1A to MMCP-1E
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.39 side-chain modification 5 variant glycoforms, differences in the electrophoretic mobility between the variant forms of MMCP-1 are probably a result of variable glycosylation Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.39 mast cell
-
 Mus musculus
-
3.4.21.39 small intestine
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.39 benzoyl-Arg-4-nitroanilide + H2O no activity with enzyme forms 1D and 1E Mus musculus benzoyl-Arg + 4-nitroaniline
-
 ?
3.4.21.39 benzoyl-L-Tyr-4-nitroanilide + H2O activity with enzyme form 1A, no activity with enzyme form 1B, 1C, 1D, 1E Mus musculus benzoyl-L-Tyr + 4-nitroaniline
-
 ?
3.4.21.39 benzyloxycarbonyl-Ala-4-nitrophenyl ester + H2O
-
 Mus musculus benzyloxycarbonyl-Ala + 4-nitrophenyl
-
 ?
3.4.21.39 benzyloxycarbonyl-Arg-4-nitrophenyl ester + H2O no activity with enzyme form 1B, 1C and 1E Mus musculus benzyloxycarbonyl-Arg + 4-nitrophenol
-
 ?
3.4.21.39 benzyloxycarbonyl-D-Ala-4-nitrophenyl ester + H2O
-
 Mus musculus benzyloxycarbonyl + D-Ala-4-nitrophenol
-
 ?
3.4.21.39 benzyloxycarbonyl-Phe-4-nitrophenyl ester + H2O
-
 Mus musculus benzyloxycarbonyl-Phe + 4-nitrophenol
-
 ?
3.4.21.39 benzyloxycarbonyl-Trp-4-nitrophenyl ester + H2O
-
 Mus musculus benzyloxycarbonyl-Trp + 4-nitrophenol
-
 ?
3.4.21.39 benzyloxycarbonyl-Tyr-4-nitrophenyl ester + H2O no activity with enzyme form 1C and 1E Mus musculus benzyloxycarbonyl-Tyr-4-nitrophenyl ester
-
 ?
3.4.21.39 succinyl-Phe-4-nitroanilide + H2O no activity with enzyme form 1C and 1D Mus musculus succinyl-Phe + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.39 dimer 1 * 32000 + 1 * 33000, enzyme form MMCP-1A, SDS-PAGE Mus musculus
3.4.21.39 monomer 1 * 30000, enzyme form MMCP-1D and MMCP-1E, SDS-PAGE Mus musculus
3.4.21.39 monomer 1 * 31000, enzyme form MMCP-1B and MMCP-1C, SDS-PAGE Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.39 1.25
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide enzyme form 1E Mus musculus
3.4.21.39 1.66
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide enzyme form 1B Mus musculus
3.4.21.39 2.33
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide enzyme form 1A Mus musculus
3.4.21.39 2.54
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide enzyme form 1D Mus musculus
3.4.21.39 2.84
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide enzyme form 1C Mus musculus