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Literature summary extracted from

  • Takao, K.; Takai, S.; Shiota, N.; Song, K.; Nishimura, K.; Ishihara, T.; Miyazaki, M.
    Lack of effect of carbohydrate depletion on some properties of human mast cell chymase (1999), Biochim. Biophys. Acta, 1427, 74-81.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.21.39 no change in susceptibility to autolysis due to deglycosylation Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.39 0.0141
-
 big endothelin deglycosylated enzyme Homo sapiens
3.4.21.39 0.0145
-
 Big endothelin-1 glycosylated enzyme Homo sapiens
3.4.21.39 0.0223
-
 angiotensin I deglycosylated enzyme Homo sapiens
3.4.21.39 0.0255
-
 angiotensin I glycosylated enzyme Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.39 30000
-
 x * 30000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.39 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.39 side-chain modification carbohydrate content is 10%. The N-glycan contributes to the stability of the enzyme but not to its functional properties Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.39
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.39 artery gastroepiploic Homo sapiens
-
3.4.21.39 mast cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.39 angiotensin I + H2O
-
 Homo sapiens angiotensin II + His-Leu
-
 ?
3.4.21.39 big endothelin + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.39 Big endothelin-1 + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.39 collagen type IV + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.39 Fibronectin + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.39 interleukin 1beta precursor + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.39 neurotensin + H2O
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.39 ? x * 30000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.39 45 50 deglycosylated enzyme Homo sapiens
3.4.21.39 50 55 glycosylated enzyme Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.39 37
-
 pH 7.4, glycosylated enzyme, stable for 24 Homo sapiens
3.4.21.39 40
-
 pH 8.0, 20 min, stable up to Homo sapiens
3.4.21.39 50
-
 pH 8.0, 20 min, about 65% loss of activity, untreated enzyme Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.39 24.7
-
 Big endothelin-1 untreated enzyme Homo sapiens
3.4.21.39 25.6
-
 Big endothelin-1 deglycosylated enzyme Homo sapiens
3.4.21.39 140
-
 angiotensin I glycosylated enzyme Homo sapiens
3.4.21.39 143
-
 angiotensin I deglycosylated enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.39 8 9.5
-
 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.39 6 8 3 h, stable Homo sapiens
3.4.21.39 7.4
-
 37°C, glycosylated enzyme, stable for 24 Homo sapiens