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Literature summary extracted from

  • Iwayama, A.; Kimura, T.; Adachi, O.; Ameyama, M.
    Crystallization and characterization of a novel aminopeptidase from Trichosporon cutaneum (1983), Agric. Biol. Chem., 47, 2483-2493.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.4.11.17 synthesis part of method for Trp-production Cutaneotrichosporon cutaneum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.11.17
-
 Cutaneotrichosporon cutaneum
3.5.1.57
-
 Cutaneotrichosporon cutaneum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.17 1,10-phenanthroline 27% inhibition at 1 mM Cutaneotrichosporon cutaneum
3.4.11.17 alpha,alpha'-dipyridyl 53% inhibition at 1 mM Cutaneotrichosporon cutaneum
3.4.11.17 monoiodoacetate 25% inhibition at 1 mM Cutaneotrichosporon cutaneum
3.4.11.17 N-ethylmaleimide 50% inhibition at 1 mM Cutaneotrichosporon cutaneum
3.5.1.57 1,10-phenanthroline weak Cutaneotrichosporon cutaneum
3.5.1.57 2,2'-dipyridyl
-
 Cutaneotrichosporon cutaneum
3.5.1.57 monoiodoacetate weak Cutaneotrichosporon cutaneum
3.5.1.57 NEM
-
 Cutaneotrichosporon cutaneum
3.5.1.57 PCMB weak Cutaneotrichosporon cutaneum
3.5.1.57 PMSF weak Cutaneotrichosporon cutaneum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.17 5.6
-
 Trp-amide
-
 Cutaneotrichosporon cutaneum
3.5.1.57 5.6
-
 L-Tryptophanamide
-
 Cutaneotrichosporon cutaneum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.17 cytosol
-
 Cutaneotrichosporon cutaneum 5829
-
3.5.1.57 cytosol
-
 Cutaneotrichosporon cutaneum 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.17 Co2+ can partially replace manganese Cutaneotrichosporon cutaneum
3.4.11.17 Mg2+ can partially replace manganese, after long pre-incubation Cutaneotrichosporon cutaneum
3.4.11.17 Mn2+ required Cutaneotrichosporon cutaneum
3.5.1.57 Co2+ some activation Cutaneotrichosporon cutaneum
3.5.1.57 Mn2+ required for activity Cutaneotrichosporon cutaneum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.17 68000
-
 4 * 68000, SDS-PAGE Cutaneotrichosporon cutaneum
3.4.11.17 270000
-
 gel filtration Cutaneotrichosporon cutaneum
3.5.1.57 68000
-
 4 * 68000, SDS-PAGE Cutaneotrichosporon cutaneum
3.5.1.57 270000
-
 gel filtration Cutaneotrichosporon cutaneum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.57 L-tryptophanamide + H2O Cutaneotrichosporon cutaneum
-
 L-tryptophan + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.17 Cutaneotrichosporon cutaneum
-
-
-
3.5.1.57 Cutaneotrichosporon cutaneum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.17
-
 Cutaneotrichosporon cutaneum
3.5.1.57
-
 Cutaneotrichosporon cutaneum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.17 1110
-
-
 Cutaneotrichosporon cutaneum
3.5.1.57 1110
-
-
 Cutaneotrichosporon cutaneum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.17 Ala-amide + H2O
-
 Cutaneotrichosporon cutaneum Ala + NH3
-
 ir
3.4.11.17 Asn + H2O
-
 Cutaneotrichosporon cutaneum Asp + NH3
-
 ir
3.4.11.17 citrulline + H2O
-
 Cutaneotrichosporon cutaneum ? + ?
-
 ir
3.4.11.17 Gln + H2O
-
 Cutaneotrichosporon cutaneum Glu + NH3
-
 ir
3.4.11.17 His-Ala + H2O
-
 Cutaneotrichosporon cutaneum His + Ala
-
 ir
3.4.11.17 His-Gly + H2O
-
 Cutaneotrichosporon cutaneum His + Gly
-
 ir
3.4.11.17 His-Leu + H2O
-
 Cutaneotrichosporon cutaneum His + Leu
-
 ir
3.4.11.17 His-Phe + H2O
-
 Cutaneotrichosporon cutaneum His + Phe
-
 ir
3.4.11.17 Leu-Ala + H2O
-
 Cutaneotrichosporon cutaneum Leu + Ala
-
 ir
3.4.11.17 Leu-amide + H2O
-
 Cutaneotrichosporon cutaneum Leu + NH3
-
 ir
3.4.11.17 Leu-Gly + H2O
-
 Cutaneotrichosporon cutaneum Leu + Gly
-
 ir
3.4.11.17 Leu-Gly-Gly + H2O
-
 Cutaneotrichosporon cutaneum Leu + Gly-Gly
-
 ir
3.4.11.17 Leu-Phe + H2O
-
 Cutaneotrichosporon cutaneum Leu + Phe
-
 ir
3.4.11.17 Leu-Trp + H2O
-
 Cutaneotrichosporon cutaneum Leu + Trp
-
 ir
3.4.11.17 Met-amide + H2O
-
 Cutaneotrichosporon cutaneum Met + NH3
-
 ir
3.4.11.17 additional information generally relatively high affinity towards peptides with N-terminal Trp Cutaneotrichosporon cutaneum ?
-
 ?
3.4.11.17 Phe-amide + H2O
-
 Cutaneotrichosporon cutaneum Phe + NH3
-
 ir
3.4.11.17 Phe-Leu + H2O
-
 Cutaneotrichosporon cutaneum Phe + Leu
-
 ir
3.4.11.17 Trp-Ala + H2O
-
 Cutaneotrichosporon cutaneum Trp + Ala
-
 ir
3.4.11.17 Trp-amide + H2O
-
 Cutaneotrichosporon cutaneum Trp + NH3
-
 ir
3.4.11.17 Trp-Glu + H2O
-
 Cutaneotrichosporon cutaneum Trp + Glu
-
 ir
3.4.11.17 Trp-Gly + H2O
-
 Cutaneotrichosporon cutaneum Trp + Gly
-
 ir
3.4.11.17 Trp-Leu + H2O
-
 Cutaneotrichosporon cutaneum Trp + Leu
-
 ir
3.4.11.17 Trp-Phe + H2O
-
 Cutaneotrichosporon cutaneum Trp + Phe
-
 ir
3.4.11.17 Trp-Trp + H2O
-
 Cutaneotrichosporon cutaneum Trp + Trp
-
 ir
3.4.11.17 Trp-Tyr + H2O
-
 Cutaneotrichosporon cutaneum Trp + Tyr
-
 ir
3.4.11.17 Tyr-amide + H2O
-
 Cutaneotrichosporon cutaneum Tyr + NH3
-
 ir
3.4.11.17 Val-Ala + H2O
-
 Cutaneotrichosporon cutaneum Val + Ala
-
 ir
3.4.11.17 Val-amide + H2O
-
 Cutaneotrichosporon cutaneum Val + NH3
-
 ir
3.4.11.17 Val-Gly-Gly + H2O
-
 Cutaneotrichosporon cutaneum Val + Gly-Gly
-
 ir
3.5.1.57 L-alaninamide + H2O 14% of the activity with L-tryptophanamide Cutaneotrichosporon cutaneum L-alanine + NH3
-
 ?
3.5.1.57 L-asparagine + H2O 22% of the activity with L-tryptophanamide Cutaneotrichosporon cutaneum L-aspartate + NH3
-
 ?
3.5.1.57 L-citrullin + H2O 22% of the activity with L-tryptophanamide Cutaneotrichosporon cutaneum ?
-
 ?
3.5.1.57 L-glutamine + H2O 18% of the activity with L-tryptophanamide Cutaneotrichosporon cutaneum L-glutamate + NH3
-
 ?
3.5.1.57 L-leucinamide + H2O 26% of the activity with L-tryptophanamide Cutaneotrichosporon cutaneum L-leucine + NH3
-
 ?
3.5.1.57 L-methioninamide + H2O 14% of the activity with L-tryptophanamide Cutaneotrichosporon cutaneum L-methionine + NH3
-
 ?
3.5.1.57 L-phenylalaninamide + H2O 47% of the activity with L-tryptophanamide Cutaneotrichosporon cutaneum L-phenylalanine + NH3
-
 ?
3.5.1.57 L-serinamide + H2O 7% of the activity with L-tryptophanamide Cutaneotrichosporon cutaneum L-serine + NH3
-
 ?
3.5.1.57 L-tryptophanamide + H2O
-
 Cutaneotrichosporon cutaneum L-tryptophan + NH3
-
 ?
3.5.1.57 L-tyrosinamide + H2O 22% of the activity with L-tryptophanamide Cutaneotrichosporon cutaneum L-tyrosine + NH3
-
 ?
3.5.1.57 L-valinamide + H2O 15% of the activity with L-tryptophanamide Cutaneotrichosporon cutaneum L-valine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.17 tetramer 4 * 68000, SDS-PAGE Cutaneotrichosporon cutaneum
3.5.1.57 tetramer 4 * 68000, SDS-PAGE Cutaneotrichosporon cutaneum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.17 40 45
-
 Cutaneotrichosporon cutaneum
3.5.1.57 40 45 crystalline enzyme Cutaneotrichosporon cutaneum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.57 35 60 crystalline enzyme, 35°C: about 75% of activity maximum, 60°C: about 35% of activity maximum Cutaneotrichosporon cutaneum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.17 55
-
 below Cutaneotrichosporon cutaneum
3.5.1.57 55
-
 crystalline enzyme, 10 min, stable Cutaneotrichosporon cutaneum
3.5.1.57 60
-
 crystalline enzyme, rapid inactivation Cutaneotrichosporon cutaneum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.17 9 9.5
-
 Cutaneotrichosporon cutaneum
3.5.1.57 9 9.5 crystalline enzyme Cutaneotrichosporon cutaneum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.57 7.5 8.5 crystalline enzyme, pH 7.5, 20 h, room temperature Cutaneotrichosporon cutaneum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.17 1
-
 N-ethylmaleimide
-
 Cutaneotrichosporon cutaneum