BRENDA - Enzyme Database

A novel aminopeptidase from Clostridium histolyticum

Kessler, E.; Yaron, A.; Biochem. Biophys. Res. Commun. 50, 405-412 (1973) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.11.13
Cu2+
-
Hathewaya histolytica
3.4.11.13
p-mercuribenzoate
-
Hathewaya histolytica
3.4.11.13
Zn2+
-
Hathewaya histolytica
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.11.13
additional information
-
additional information
-
Hathewaya histolytica
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.4.11.13
extracellular
-
Hathewaya histolytica
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.11.13
Cd2+
-
Hathewaya histolytica
3.4.11.13
Co2+
-
Hathewaya histolytica
3.4.11.13
Mn2+
-
Hathewaya histolytica
3.4.11.13
Ni2+
-
Hathewaya histolytica
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.11.13
340000
-
gel filtration
Hathewaya histolytica
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.4.11.13
Peptides + H2O
Hathewaya histolytica
-
?
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.4.11.13
Hathewaya histolytica
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.4.11.13
-
Hathewaya histolytica
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.11.13
10.5
-
-
Hathewaya histolytica
Storage Stability
EC Number
Storage Stability
Organism
3.4.11.13
-20°C, 0.05 M phosphate buffer or 0.05 M Na-acetate buffer, pH 5.6, 1 mM EDTA, several years, little loss of activity, can be restored by treatment with 2.5 mM dithiothreitol
Hathewaya histolytica
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.4.11.13
(Ala)3 + H2O
-
81197
Hathewaya histolytica
Ala + Ala-Ala
-
81197
Hathewaya histolytica
ir
3.4.11.13
(Ala)4 + H2O
-
81197
Hathewaya histolytica
Ala + (Ala)3
or Ala-Ala + Ala-Ala
81197
Hathewaya histolytica
ir
3.4.11.13
(Ala)4-NH2 + H2O
-
81197
Hathewaya histolytica
Ala + (Ala)3-NH2
or Ala-Ala + Ala-Ala-NH2 or (Ala)3 + Ala-NH2
81197
Hathewaya histolytica
ir
3.4.11.13
(Pro-Gly-Pro)4-6 + H2O
-
81197
Hathewaya histolytica
Pro + Gly-Pro-(Pro-Gly-Pro)3-5
-
81197
Hathewaya histolytica
ir
3.4.11.13
(Pro-Gly-Pro)4-6-OMe + H2O
-
81197
Hathewaya histolytica
Pro + Gly-Pro-(Pro-Gly-Pro)3-5-OMe
-
81197
Hathewaya histolytica
ir
3.4.11.13
Ala-Ala-Phe-Ala + H2O
-
81197
Hathewaya histolytica
Ala + Ala-Phe-Ala
or Ala-Ala + Phe-Ala
81197
Hathewaya histolytica
ir
3.4.11.13
Ala-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Ala + Gly-Gly
-
81197
Hathewaya histolytica
ir
3.4.11.13
Ala-Lys + H2O
-
81197
Hathewaya histolytica
Ala + Lys
-
81197
Hathewaya histolytica
ir
3.4.11.13
Gly-Gly-Phe + H2O
-
81197
Hathewaya histolytica
Gly + Gly-Phe
-
81197
Hathewaya histolytica
ir
3.4.11.13
Gly-Gly-Phe-Ala + H2O
-
81197
Hathewaya histolytica
Gly + Gly-Phe-Ala
or Gly-Gly + Phe-Ala
81197
Hathewaya histolytica
ir
3.4.11.13
Gly-Phe-Ala + H2O
-
81197
Hathewaya histolytica
Gly + Phe-Ala
-
81197
Hathewaya histolytica
ir
3.4.11.13
Hyp-Ala-NH2 + H2O
-
81197
Hathewaya histolytica
Hyp-Ala + NH3
-
81197
Hathewaya histolytica
ir
3.4.11.13
Leu-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Leu + Gly-Gly
-
81197
Hathewaya histolytica
ir
3.4.11.13
Met-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Met + Gly-Gly
-
81197
Hathewaya histolytica
ir
3.4.11.13
Met-Met + H2O
-
81197
Hathewaya histolytica
Met + Met
-
81197
Hathewaya histolytica
ir
3.4.11.13
Peptides + H2O
-
81197
Hathewaya histolytica
?
-
-
-
?
3.4.11.13
Phe-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Phe + Gly-Gly
-
81197
Hathewaya histolytica
ir
3.4.11.13
Pro-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Pro + Gly-Gly
-
81197
Hathewaya histolytica
ir
3.4.11.13
Pro-Gly-Pro + H2O
-
81197
Hathewaya histolytica
Pro + Gly-Pro
-
-
-
?
3.4.11.13
Pro-Gly-Pro-Pro + H2O
-
81197
Hathewaya histolytica
Pro + Gly-Pro-Pro
-
81197
Hathewaya histolytica
ir
3.4.11.13
Pro-Leu + H2O
-
81197
Hathewaya histolytica
Pro + Leu
-
81197
Hathewaya histolytica
ir
3.4.11.13
Pro-Phe + H2O
-
81197
Hathewaya histolytica
Pro + Phe
-
81197
Hathewaya histolytica
ir
3.4.11.13
Ser-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Ser + Gly-Gly
-
81197
Hathewaya histolytica
ir
3.4.11.13
Val-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Val + Gly-Gly
-
81197
Hathewaya histolytica
ir
Subunits
EC Number
Subunits
Commentary
Organism
3.4.11.13
hexamer
6 * 51000-59000, SDS-PAGE
Hathewaya histolytica
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.4.11.13
additional information
-
additional information
-
Hathewaya histolytica
3.4.11.13
276
-
Val-Gly-Gly
-
Hathewaya histolytica
3.4.11.13
337
-
Pro-Gly-Pro-Pro
-
Hathewaya histolytica
3.4.11.13
561
-
Pro-Gly-Pro
-
Hathewaya histolytica
3.4.11.13
667
-
Phe-Gly-Gly
-
Hathewaya histolytica
3.4.11.13
917
-
Leu-Gly-Gly
-
Hathewaya histolytica
3.4.11.13
1360
-
Pro-Gly-Gly
-
Hathewaya histolytica
3.4.11.13
1830
-
Ala-Gly-Gly
-
Hathewaya histolytica
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.11.13
8.6
-
assay at
Hathewaya histolytica
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.11.13
Cu2+
-
Hathewaya histolytica
3.4.11.13
p-mercuribenzoate
-
Hathewaya histolytica
3.4.11.13
Zn2+
-
Hathewaya histolytica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.11.13
additional information
-
additional information
-
Hathewaya histolytica
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.4.11.13
extracellular
-
Hathewaya histolytica
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.11.13
Cd2+
-
Hathewaya histolytica
3.4.11.13
Co2+
-
Hathewaya histolytica
3.4.11.13
Mn2+
-
Hathewaya histolytica
3.4.11.13
Ni2+
-
Hathewaya histolytica
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.11.13
340000
-
gel filtration
Hathewaya histolytica
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.4.11.13
Peptides + H2O
Hathewaya histolytica
-
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.11.13
-
Hathewaya histolytica
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.11.13
10.5
-
-
Hathewaya histolytica
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.4.11.13
-20°C, 0.05 M phosphate buffer or 0.05 M Na-acetate buffer, pH 5.6, 1 mM EDTA, several years, little loss of activity, can be restored by treatment with 2.5 mM dithiothreitol
Hathewaya histolytica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.4.11.13
(Ala)3 + H2O
-
81197
Hathewaya histolytica
Ala + Ala-Ala
-
81197
Hathewaya histolytica
ir
3.4.11.13
(Ala)4 + H2O
-
81197
Hathewaya histolytica
Ala + (Ala)3
or Ala-Ala + Ala-Ala
81197
Hathewaya histolytica
ir
3.4.11.13
(Ala)4-NH2 + H2O
-
81197
Hathewaya histolytica
Ala + (Ala)3-NH2
or Ala-Ala + Ala-Ala-NH2 or (Ala)3 + Ala-NH2
81197
Hathewaya histolytica
ir
3.4.11.13
(Pro-Gly-Pro)4-6 + H2O
-
81197
Hathewaya histolytica
Pro + Gly-Pro-(Pro-Gly-Pro)3-5
-
81197
Hathewaya histolytica
ir
3.4.11.13
(Pro-Gly-Pro)4-6-OMe + H2O
-
81197
Hathewaya histolytica
Pro + Gly-Pro-(Pro-Gly-Pro)3-5-OMe
-
81197
Hathewaya histolytica
ir
3.4.11.13
Ala-Ala-Phe-Ala + H2O
-
81197
Hathewaya histolytica
Ala + Ala-Phe-Ala
or Ala-Ala + Phe-Ala
81197
Hathewaya histolytica
ir
3.4.11.13
Ala-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Ala + Gly-Gly
-
81197
Hathewaya histolytica
ir
3.4.11.13
Ala-Lys + H2O
-
81197
Hathewaya histolytica
Ala + Lys
-
81197
Hathewaya histolytica
ir
3.4.11.13
Gly-Gly-Phe + H2O
-
81197
Hathewaya histolytica
Gly + Gly-Phe
-
81197
Hathewaya histolytica
ir
3.4.11.13
Gly-Gly-Phe-Ala + H2O
-
81197
Hathewaya histolytica
Gly + Gly-Phe-Ala
or Gly-Gly + Phe-Ala
81197
Hathewaya histolytica
ir
3.4.11.13
Gly-Phe-Ala + H2O
-
81197
Hathewaya histolytica
Gly + Phe-Ala
-
81197
Hathewaya histolytica
ir
3.4.11.13
Hyp-Ala-NH2 + H2O
-
81197
Hathewaya histolytica
Hyp-Ala + NH3
-
81197
Hathewaya histolytica
ir
3.4.11.13
Leu-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Leu + Gly-Gly
-
81197
Hathewaya histolytica
ir
3.4.11.13
Met-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Met + Gly-Gly
-
81197
Hathewaya histolytica
ir
3.4.11.13
Met-Met + H2O
-
81197
Hathewaya histolytica
Met + Met
-
81197
Hathewaya histolytica
ir
3.4.11.13
Peptides + H2O
-
81197
Hathewaya histolytica
?
-
-
-
?
3.4.11.13
Phe-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Phe + Gly-Gly
-
81197
Hathewaya histolytica
ir
3.4.11.13
Pro-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Pro + Gly-Gly
-
81197
Hathewaya histolytica
ir
3.4.11.13
Pro-Gly-Pro + H2O
-
81197
Hathewaya histolytica
Pro + Gly-Pro
-
-
-
?
3.4.11.13
Pro-Gly-Pro-Pro + H2O
-
81197
Hathewaya histolytica
Pro + Gly-Pro-Pro
-
81197
Hathewaya histolytica
ir
3.4.11.13
Pro-Leu + H2O
-
81197
Hathewaya histolytica
Pro + Leu
-
81197
Hathewaya histolytica
ir
3.4.11.13
Pro-Phe + H2O
-
81197
Hathewaya histolytica
Pro + Phe
-
81197
Hathewaya histolytica
ir
3.4.11.13
Ser-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Ser + Gly-Gly
-
81197
Hathewaya histolytica
ir
3.4.11.13
Val-Gly-Gly + H2O
-
81197
Hathewaya histolytica
Val + Gly-Gly
-
81197
Hathewaya histolytica
ir
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.4.11.13
hexamer
6 * 51000-59000, SDS-PAGE
Hathewaya histolytica
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.4.11.13
additional information
-
additional information
-
Hathewaya histolytica
3.4.11.13
276
-
Val-Gly-Gly
-
Hathewaya histolytica
3.4.11.13
337
-
Pro-Gly-Pro-Pro
-
Hathewaya histolytica
3.4.11.13
561
-
Pro-Gly-Pro
-
Hathewaya histolytica
3.4.11.13
667
-
Phe-Gly-Gly
-
Hathewaya histolytica
3.4.11.13
917
-
Leu-Gly-Gly
-
Hathewaya histolytica
3.4.11.13
1360
-
Pro-Gly-Gly
-
Hathewaya histolytica
3.4.11.13
1830
-
Ala-Gly-Gly
-
Hathewaya histolytica
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.11.13
8.6
-
assay at
Hathewaya histolytica