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Literature summary extracted from

  • Izawa, N.; Ishikawa, S.; Tanokura, T.; Ohta, K.; Hayashi, K.
    Purification and characterization of Aeromonas caviae aminopeptidase possessing dibittering activity. (1997), J. Agric. Food Chem., 45, 4897-4902.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.11.10 nutrition debittering of casein- and soyprotein-derived peptide solutions Aeromonas caviae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.10 1,10-phenanthroline 99% inhibition at 1 mM Aeromonas caviae
3.4.11.10 bestatin 99% inhibition at 0.05 mM Aeromonas caviae
3.4.11.10 Co2+
-
 Aeromonas caviae
3.4.11.10 Cu2+
-
 Aeromonas caviae
3.4.11.10 EDTA 10% inhibition at 1 mM Aeromonas caviae
3.4.11.10 Mn2+
-
 Aeromonas caviae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.10 0.03
-
 Ile-Phe
-
 Aeromonas caviae
3.4.11.10 0.05
-
 Leu-Phe
-
 Aeromonas caviae
3.4.11.10 0.07
-
 L-Val-L-Phe
-
 Aeromonas caviae
3.4.11.10 0.1
-
 Leu-beta-naphthylamide
-
 Aeromonas caviae
3.4.11.10 0.19
-
 Val-beta-naphthylamide
-
 Aeromonas caviae
3.4.11.10 0.52
-
 Phe-beta-naphthylamide
-
 Aeromonas caviae
3.4.11.10 0.96
-
 Met-beta-naphthylamide
-
 Aeromonas caviae
3.4.11.10 1.1
-
 Met-Phe
-
 Aeromonas caviae
3.4.11.10 2.1
-
 Phe-Phe
-
 Aeromonas caviae
3.4.11.10 4.6
-
 Arg-Phe
-
 Aeromonas caviae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.10 extracellular
-
 Aeromonas caviae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.10 Zn2+
-
 Aeromonas caviae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.10 29500
-
 MALDI-TOF mass spectrometry Aeromonas caviae
3.4.11.10 31000
-
 1 * 31000, SDS-PAGE Aeromonas caviae

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.10 Aeromonas caviae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.10
-
 Aeromonas caviae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.10 55
-
-
 Aeromonas caviae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.10 Arg-Phe + H2O
-
 Aeromonas caviae Arg + Phe
-
 ir
3.4.11.10 Glu-Phe + H2O
-
 Aeromonas caviae Glu + Phe
-
 ir
3.4.11.10 Ile-Phe + H2O
-
 Aeromonas caviae Ile + Phe
-
 ir
3.4.11.10 L-Val-L-Phe + H2O
-
 Aeromonas caviae L-Val + L-Phe
-
 ?
3.4.11.10 Leu-beta-naphthylamide + H2O
-
 Aeromonas caviae Leu + beta-naphthylamine
-
 ir
3.4.11.10 Leu-Phe + H2O
-
 Aeromonas caviae Leu + Phe
-
 ir
3.4.11.10 Met-beta-naphthylamide + H2O
-
 Aeromonas caviae Met + beta-naphthylamine
-
 ir
3.4.11.10 Met-Phe + H2O
-
 Aeromonas caviae Met + Phe
-
 ir
3.4.11.10 additional information effect of adjacent amino acids on specificity Aeromonas caviae ?
-
 ?
3.4.11.10 Phe-beta-naphthylamide + H2O
-
 Aeromonas caviae Phe + beta-naphthylamine
-
 ir
3.4.11.10 Phe-Phe + H2O
-
 Aeromonas caviae Phe + Phe
-
 ir
3.4.11.10 Val-beta-naphthylamide + H2O
-
 Aeromonas caviae Val + beta-naphthylamine
-
 ir

Subunits

EC Number Subunits Comment Organism
3.4.11.10 monomer 1 * 31000, SDS-PAGE Aeromonas caviae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.10 50
-
-
 Aeromonas caviae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.10 40
-
 below, 30 min Aeromonas caviae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.10 additional information
-
 additional information penultimative substituents influence rate of hydrolysis Aeromonas caviae
3.4.11.10 0.001
-
 Glu-Phe
-
 Aeromonas caviae
3.4.11.10 0.26
-
 Val-beta-naphthylamide
-
 Aeromonas caviae
3.4.11.10 1.3
-
 L-Val-L-Phe
-
 Aeromonas caviae
3.4.11.10 6
-
 Arg-Phe
-
 Aeromonas caviae
3.4.11.10 6.3
-
 Phe-beta-naphthylamide
-
 Aeromonas caviae
3.4.11.10 8.2
-
 Met-beta-naphthylamide
-
 Aeromonas caviae
3.4.11.10 10
-
 Ile-Phe
-
 Aeromonas caviae
3.4.11.10 22
-
 Met-Phe
-
 Aeromonas caviae
3.4.11.10 38
-
 Leu-beta-naphthylamide
-
 Aeromonas caviae
3.4.11.10 41
-
 Leu-Phe
-
 Aeromonas caviae
3.4.11.10 158
-
 Phe-Phe
-
 Aeromonas caviae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.10 8.5
-
-
 Aeromonas caviae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.10 8 9 30 min Aeromonas caviae