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Literature summary extracted from

  • Prescott, J.M.; Wilkes, S.H.
    Aeromonas aminopeptidase (1976), Methods Enzymol., 45, 530-543.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.11.1 no inactivation Vibrio proteolyticus
3.4.11.1 urea 8 M, partial inactivation Vibrio proteolyticus
3.4.11.10 only partially inactivated in 8 M urea Vibrio proteolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 (CH3)2CHCH2CH(NH2)CO-X X: e.g. succinamide,-CH3,-CH2Cl Vibrio proteolyticus
3.4.11.1 1,10-phenanthroline
-
Vibrio proteolyticus
3.4.11.10 1,10-phenanthroline
-
Vibrio proteolyticus
3.4.11.10 EDTA
-
Vibrio proteolyticus
3.4.11.10 Leu-bromomethyl ketone succinamic acid derivative, Ki: 0.0069 mM Vibrio proteolyticus
3.4.11.10 Leu-methyl ketone derivatives, Ki: 0.14 - 0.17 mM Vibrio proteolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 0.25
-
L-Leu-2-naphthylamide similar value Vibrio proteolyticus
3.4.11.1 0.51
-
L-Leu-L-Val similar value Vibrio proteolyticus
3.4.11.1 4.02
-
leucinamide similar value Vibrio proteolyticus
3.4.11.1 7.2
-
L-Leu-Gly similar value Vibrio proteolyticus
3.4.11.1 8.5
-
L-Leu methyl ester
-
Vibrio proteolyticus
3.4.11.10 additional information
-
additional information amides and dipeptides Vibrio proteolyticus
3.4.11.10 0.019
-
L-leucine 4-nitroanilide
-
Vibrio proteolyticus
3.4.11.10 0.18
-
Leu-Leu
-
Vibrio proteolyticus
3.4.11.10 0.35
-
Leu-Met
-
Vibrio proteolyticus
3.4.11.10 0.38
-
Leu-Ile
-
Vibrio proteolyticus
3.4.11.10 0.39
-
Leu-Arg
-
Vibrio proteolyticus
3.4.11.10 0.43
-
Leu-beta-naphthylamide
-
Vibrio proteolyticus
3.4.11.10 0.64
-
Leu-Val
-
Vibrio proteolyticus
3.4.11.10 0.86
-
Leu-Phe
-
Vibrio proteolyticus
3.4.11.10 0.96
-
Leu-Trp
-
Vibrio proteolyticus
3.4.11.10 1
-
Leu-Ala
-
Vibrio proteolyticus
3.4.11.10 1.5
-
Leu-Tyr
-
Vibrio proteolyticus
3.4.11.10 3.9
-
Phe-methyl ester
-
Vibrio proteolyticus
3.4.11.10 5.1
-
Leu-amide
-
Vibrio proteolyticus
3.4.11.10 6.3
-
norleucinamide
-
Vibrio proteolyticus
3.4.11.10 8.5
-
Leu-methyl ester
-
Vibrio proteolyticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.1 extracellular
-
Vibrio proteolyticus
-
-
3.4.11.10 extracellular
-
Vibrio proteolyticus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Zn2+ 4-6 gatom per mol Vibrio proteolyticus
3.4.11.10 Co2+
-
Vibrio proteolyticus
3.4.11.10 Mn2+
-
Vibrio proteolyticus
3.4.11.10 Zn2+
-
Vibrio proteolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 29500
-
calculation from sedimentation and diffusion constants Vibrio proteolyticus
3.4.11.10 28480
-
sedimentation equilibrium Vibrio proteolyticus
3.4.11.10 29500
-
sedimentation velocity Vibrio proteolyticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.1 additional information Vibrio proteolyticus
-
?
-
?
3.4.11.1 Proteins + H2O Vibrio proteolyticus glucagon ?
-
?
3.4.11.10 Peptides + H2O Vibrio proteolyticus
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Vibrio proteolyticus
-
-
-
3.4.11.10 Vibrio proteolyticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1
-
Vibrio proteolyticus
3.4.11.10
-
Vibrio proteolyticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 culture supernatant
-
Vibrio proteolyticus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.1 additional information
-
assay methods Vibrio proteolyticus
3.4.11.10 35.5
-
-
Vibrio proteolyticus

Storage Stability

EC Number Storage Stability Organism
3.4.11.1 -20°C, apoenzyme or active enzyme, several years Vibrio proteolyticus
3.4.11.10 -20°C, 10 M tricine buffer, pH 8.0-8.5, several years, little loss of activity Vibrio proteolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 L-amino acid-peptide + H2O leucyl-dipeptides Vibrio proteolyticus amino acid + peptide
-
?
3.4.11.1 L-amino acid-peptide + H2O Leu-Met Vibrio proteolyticus amino acid + peptide
-
?
3.4.11.1 L-amino acid-peptide + H2O Pro-Phe-Lys Vibrio proteolyticus amino acid + peptide
-
?
3.4.11.1 L-Leu methyl ester
-
Vibrio proteolyticus ?
-
?
3.4.11.1 L-Leu-2-naphthylamide + H2O
-
Vibrio proteolyticus L-Leu + 2-naphthylamine
-
?
3.4.11.1 L-Leu-Gly + H2O
-
Vibrio proteolyticus L-Leu + Gly
-
?
3.4.11.1 L-leucine amide + H2O
-
Vibrio proteolyticus L-leucine + NH3
-
?
3.4.11.1 Leu-Val + H2O
-
Vibrio proteolyticus Leu + Val
-
?
3.4.11.1 additional information
-
Vibrio proteolyticus ?
-
?
3.4.11.1 additional information esterase activity Vibrio proteolyticus ?
-
?
3.4.11.1 Proteins + H2O glucagon Vibrio proteolyticus ?
-
?
3.4.11.10 Leu-4-nitroanilide + H2O
-
Vibrio proteolyticus Leu + 4-nitroaniline
-
ir
3.4.11.10 Leu-Ala + H2O
-
Vibrio proteolyticus Leu + Ala
-
ir
3.4.11.10 Leu-amide + H2O
-
Vibrio proteolyticus Leu + NH3
-
ir
3.4.11.10 Leu-Arg + H2O
-
Vibrio proteolyticus Leu + Arg
-
ir
3.4.11.10 Leu-beta-naphthylamide + H2O
-
Vibrio proteolyticus Leu + beta-naphthylamine
-
ir
3.4.11.10 Leu-Ile + H2O
-
Vibrio proteolyticus Leu + Ile
-
ir
3.4.11.10 Leu-Leu + H2O
-
Vibrio proteolyticus Leu + Leu
-
ir
3.4.11.10 Leu-Met + H2O
-
Vibrio proteolyticus Leu + Met
-
ir
3.4.11.10 Leu-methyl ester + H2O
-
Vibrio proteolyticus Leu + methanol
-
ir
3.4.11.10 Leu-Phe + H2O
-
Vibrio proteolyticus Leu + Phe
-
ir
3.4.11.10 Leu-Trp + H2O
-
Vibrio proteolyticus Leu + Trp
-
ir
3.4.11.10 Leu-Tyr + H2O
-
Vibrio proteolyticus Leu + Tyr
-
ir
3.4.11.10 Leu-Val + H2O
-
Vibrio proteolyticus Leu + Val
-
ir
3.4.11.10 additional information also cleaved: gastrin peptide (carboxyterminal), various oligopeptides, Met-Lys-bradykinin, adrenocorticotropic hormone, glucagon, aminoethylated B-chain insulin, sperm whale myoglobin, ribunuclease, beta-lactoglobulin, egg white lysozyme, aminoethylated lysozyme Vibrio proteolyticus ?
-
?
3.4.11.10 norleucinamide + H2O
-
Vibrio proteolyticus norleucine + NH3
-
ir
3.4.11.10 Peptides + H2O
-
Vibrio proteolyticus ?
-
?
3.4.11.10 Phe-Asp-Ser-Ala-Val + H2O
-
Vibrio proteolyticus Phe + Asp-Ser-Ala-Val
-
ir
3.4.11.10 Phe-methyl ester + H2O
-
Vibrio proteolyticus Phe + methanol
-
ir
3.4.11.10 Pro-Phe-Gly-Lys + H2O
-
Vibrio proteolyticus Pro + Phe-Gly-Lys
-
ir
3.4.11.10 Pro-Phe-Pro + H2O
-
Vibrio proteolyticus Pro + Phe-Pro
-
ir

Subunits

EC Number Subunits Comment Organism
3.4.11.10 monomer SDS-PAGE Vibrio proteolyticus
3.4.11.10 More amino acid composition Vibrio proteolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.10 25
-
assay at Vibrio proteolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.1 70
-
several h Vibrio proteolyticus
3.4.11.10 70
-
below, but tolerates exposure up to a few hours Vibrio proteolyticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.10 8.3
-
Leu-Leu
-
Vibrio proteolyticus
3.4.11.10 15
-
Leu-Ala
-
Vibrio proteolyticus
3.4.11.10 15
-
Leu-Val
-
Vibrio proteolyticus
3.4.11.10 16
-
Leu-Ile
-
Vibrio proteolyticus
3.4.11.10 29.6
-
Leu-beta-naphthylamide
-
Vibrio proteolyticus
3.4.11.10 39
-
Leu-Arg
-
Vibrio proteolyticus
3.4.11.10 42
-
Phe-methyl ester
-
Vibrio proteolyticus
3.4.11.10 53
-
Leu-Met
-
Vibrio proteolyticus
3.4.11.10 58
-
Leu-Trp
-
Vibrio proteolyticus
3.4.11.10 60
-
norleucinamide
-
Vibrio proteolyticus
3.4.11.10 67
-
L-leucine 4-nitroanilide
-
Vibrio proteolyticus
3.4.11.10 72
-
Leu-Phe
-
Vibrio proteolyticus
3.4.11.10 85
-
Leu-Tyr
-
Vibrio proteolyticus
3.4.11.10 145
-
Leu-methyl ester
-
Vibrio proteolyticus
3.4.11.10 220
-
Leu-amide
-
Vibrio proteolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 8 8.5
-
Vibrio proteolyticus
3.4.11.10 8
-
assay at Vibrio proteolyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.1 8 8.5
-
Vibrio proteolyticus
3.4.11.10 8 8.5
-
Vibrio proteolyticus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.10 0.0002
-
Leu-bromomethyl ketone
-
Vibrio proteolyticus