EC Number | General Stability | Organism |
---|---|---|
3.4.11.1 | no inactivation | Vibrio proteolyticus |
3.4.11.1 | urea 8 M, partial inactivation | Vibrio proteolyticus |
3.4.11.10 | only partially inactivated in 8 M urea | Vibrio proteolyticus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.1 | (CH3)2CHCH2CH(NH2)CO-X | X: e.g. succinamide,-CH3,-CH2Cl | Vibrio proteolyticus | |
3.4.11.1 | 1,10-phenanthroline | - |
Vibrio proteolyticus | |
3.4.11.10 | 1,10-phenanthroline | - |
Vibrio proteolyticus | |
3.4.11.10 | EDTA | - |
Vibrio proteolyticus | |
3.4.11.10 | Leu-bromomethyl ketone | succinamic acid derivative, Ki: 0.0069 mM | Vibrio proteolyticus | |
3.4.11.10 | Leu-methyl ketone | derivatives, Ki: 0.14 - 0.17 mM | Vibrio proteolyticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.1 | 0.25 | - |
L-Leu-2-naphthylamide | similar value | Vibrio proteolyticus | |
3.4.11.1 | 0.51 | - |
L-Leu-L-Val | similar value | Vibrio proteolyticus | |
3.4.11.1 | 4.02 | - |
leucinamide | similar value | Vibrio proteolyticus | |
3.4.11.1 | 7.2 | - |
L-Leu-Gly | similar value | Vibrio proteolyticus | |
3.4.11.1 | 8.5 | - |
L-Leu methyl ester | - |
Vibrio proteolyticus | |
3.4.11.10 | additional information | - |
additional information | amides and dipeptides | Vibrio proteolyticus | |
3.4.11.10 | 0.019 | - |
L-leucine 4-nitroanilide | - |
Vibrio proteolyticus | |
3.4.11.10 | 0.18 | - |
Leu-Leu | - |
Vibrio proteolyticus | |
3.4.11.10 | 0.35 | - |
Leu-Met | - |
Vibrio proteolyticus | |
3.4.11.10 | 0.38 | - |
Leu-Ile | - |
Vibrio proteolyticus | |
3.4.11.10 | 0.39 | - |
Leu-Arg | - |
Vibrio proteolyticus | |
3.4.11.10 | 0.43 | - |
Leu-beta-naphthylamide | - |
Vibrio proteolyticus | |
3.4.11.10 | 0.64 | - |
Leu-Val | - |
Vibrio proteolyticus | |
3.4.11.10 | 0.86 | - |
Leu-Phe | - |
Vibrio proteolyticus | |
3.4.11.10 | 0.96 | - |
Leu-Trp | - |
Vibrio proteolyticus | |
3.4.11.10 | 1 | - |
Leu-Ala | - |
Vibrio proteolyticus | |
3.4.11.10 | 1.5 | - |
Leu-Tyr | - |
Vibrio proteolyticus | |
3.4.11.10 | 3.9 | - |
Phe-methyl ester | - |
Vibrio proteolyticus | |
3.4.11.10 | 5.1 | - |
Leu-amide | - |
Vibrio proteolyticus | |
3.4.11.10 | 6.3 | - |
norleucinamide | - |
Vibrio proteolyticus | |
3.4.11.10 | 8.5 | - |
Leu-methyl ester | - |
Vibrio proteolyticus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.11.1 | extracellular | - |
Vibrio proteolyticus | - |
- |
3.4.11.10 | extracellular | - |
Vibrio proteolyticus | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.1 | Zn2+ | 4-6 gatom per mol | Vibrio proteolyticus | |
3.4.11.10 | Co2+ | - |
Vibrio proteolyticus | |
3.4.11.10 | Mn2+ | - |
Vibrio proteolyticus | |
3.4.11.10 | Zn2+ | - |
Vibrio proteolyticus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.11.1 | 29500 | - |
calculation from sedimentation and diffusion constants | Vibrio proteolyticus |
3.4.11.10 | 28480 | - |
sedimentation equilibrium | Vibrio proteolyticus |
3.4.11.10 | 29500 | - |
sedimentation velocity | Vibrio proteolyticus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.11.1 | additional information | Vibrio proteolyticus | - |
? | - |
? | |
3.4.11.1 | Proteins + H2O | Vibrio proteolyticus | glucagon | ? | - |
? | |
3.4.11.10 | Peptides + H2O | Vibrio proteolyticus | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.11.1 | Vibrio proteolyticus | - |
- |
- |
3.4.11.10 | Vibrio proteolyticus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.11.1 | - |
Vibrio proteolyticus |
3.4.11.10 | - |
Vibrio proteolyticus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.11.1 | culture supernatant | - |
Vibrio proteolyticus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.11.1 | additional information | - |
assay methods | Vibrio proteolyticus |
3.4.11.10 | 35.5 | - |
- |
Vibrio proteolyticus |
EC Number | Storage Stability | Organism |
---|---|---|
3.4.11.1 | -20°C, apoenzyme or active enzyme, several years | Vibrio proteolyticus |
3.4.11.10 | -20°C, 10 M tricine buffer, pH 8.0-8.5, several years, little loss of activity | Vibrio proteolyticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.11.1 | L-amino acid-peptide + H2O | leucyl-dipeptides | Vibrio proteolyticus | amino acid + peptide | - |
? | |
3.4.11.1 | L-amino acid-peptide + H2O | Leu-Met | Vibrio proteolyticus | amino acid + peptide | - |
? | |
3.4.11.1 | L-amino acid-peptide + H2O | Pro-Phe-Lys | Vibrio proteolyticus | amino acid + peptide | - |
? | |
3.4.11.1 | L-Leu methyl ester | - |
Vibrio proteolyticus | ? | - |
? | |
3.4.11.1 | L-Leu-2-naphthylamide + H2O | - |
Vibrio proteolyticus | L-Leu + 2-naphthylamine | - |
? | |
3.4.11.1 | L-Leu-Gly + H2O | - |
Vibrio proteolyticus | L-Leu + Gly | - |
? | |
3.4.11.1 | L-leucine amide + H2O | - |
Vibrio proteolyticus | L-leucine + NH3 | - |
? | |
3.4.11.1 | Leu-Val + H2O | - |
Vibrio proteolyticus | Leu + Val | - |
? | |
3.4.11.1 | additional information | - |
Vibrio proteolyticus | ? | - |
? | |
3.4.11.1 | additional information | esterase activity | Vibrio proteolyticus | ? | - |
? | |
3.4.11.1 | Proteins + H2O | glucagon | Vibrio proteolyticus | ? | - |
? | |
3.4.11.10 | Leu-4-nitroanilide + H2O | - |
Vibrio proteolyticus | Leu + 4-nitroaniline | - |
ir | |
3.4.11.10 | Leu-Ala + H2O | - |
Vibrio proteolyticus | Leu + Ala | - |
ir | |
3.4.11.10 | Leu-amide + H2O | - |
Vibrio proteolyticus | Leu + NH3 | - |
ir | |
3.4.11.10 | Leu-Arg + H2O | - |
Vibrio proteolyticus | Leu + Arg | - |
ir | |
3.4.11.10 | Leu-beta-naphthylamide + H2O | - |
Vibrio proteolyticus | Leu + beta-naphthylamine | - |
ir | |
3.4.11.10 | Leu-Ile + H2O | - |
Vibrio proteolyticus | Leu + Ile | - |
ir | |
3.4.11.10 | Leu-Leu + H2O | - |
Vibrio proteolyticus | Leu + Leu | - |
ir | |
3.4.11.10 | Leu-Met + H2O | - |
Vibrio proteolyticus | Leu + Met | - |
ir | |
3.4.11.10 | Leu-methyl ester + H2O | - |
Vibrio proteolyticus | Leu + methanol | - |
ir | |
3.4.11.10 | Leu-Phe + H2O | - |
Vibrio proteolyticus | Leu + Phe | - |
ir | |
3.4.11.10 | Leu-Trp + H2O | - |
Vibrio proteolyticus | Leu + Trp | - |
ir | |
3.4.11.10 | Leu-Tyr + H2O | - |
Vibrio proteolyticus | Leu + Tyr | - |
ir | |
3.4.11.10 | Leu-Val + H2O | - |
Vibrio proteolyticus | Leu + Val | - |
ir | |
3.4.11.10 | additional information | also cleaved: gastrin peptide (carboxyterminal), various oligopeptides, Met-Lys-bradykinin, adrenocorticotropic hormone, glucagon, aminoethylated B-chain insulin, sperm whale myoglobin, ribunuclease, beta-lactoglobulin, egg white lysozyme, aminoethylated lysozyme | Vibrio proteolyticus | ? | - |
? | |
3.4.11.10 | norleucinamide + H2O | - |
Vibrio proteolyticus | norleucine + NH3 | - |
ir | |
3.4.11.10 | Peptides + H2O | - |
Vibrio proteolyticus | ? | - |
? | |
3.4.11.10 | Phe-Asp-Ser-Ala-Val + H2O | - |
Vibrio proteolyticus | Phe + Asp-Ser-Ala-Val | - |
ir | |
3.4.11.10 | Phe-methyl ester + H2O | - |
Vibrio proteolyticus | Phe + methanol | - |
ir | |
3.4.11.10 | Pro-Phe-Gly-Lys + H2O | - |
Vibrio proteolyticus | Pro + Phe-Gly-Lys | - |
ir | |
3.4.11.10 | Pro-Phe-Pro + H2O | - |
Vibrio proteolyticus | Pro + Phe-Pro | - |
ir |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.11.10 | monomer | SDS-PAGE | Vibrio proteolyticus |
3.4.11.10 | More | amino acid composition | Vibrio proteolyticus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.11.10 | 25 | - |
assay at | Vibrio proteolyticus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.11.1 | 70 | - |
several h | Vibrio proteolyticus |
3.4.11.10 | 70 | - |
below, but tolerates exposure up to a few hours | Vibrio proteolyticus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.10 | 8.3 | - |
Leu-Leu | - |
Vibrio proteolyticus | |
3.4.11.10 | 15 | - |
Leu-Ala | - |
Vibrio proteolyticus | |
3.4.11.10 | 15 | - |
Leu-Val | - |
Vibrio proteolyticus | |
3.4.11.10 | 16 | - |
Leu-Ile | - |
Vibrio proteolyticus | |
3.4.11.10 | 29.6 | - |
Leu-beta-naphthylamide | - |
Vibrio proteolyticus | |
3.4.11.10 | 39 | - |
Leu-Arg | - |
Vibrio proteolyticus | |
3.4.11.10 | 42 | - |
Phe-methyl ester | - |
Vibrio proteolyticus | |
3.4.11.10 | 53 | - |
Leu-Met | - |
Vibrio proteolyticus | |
3.4.11.10 | 58 | - |
Leu-Trp | - |
Vibrio proteolyticus | |
3.4.11.10 | 60 | - |
norleucinamide | - |
Vibrio proteolyticus | |
3.4.11.10 | 67 | - |
L-leucine 4-nitroanilide | - |
Vibrio proteolyticus | |
3.4.11.10 | 72 | - |
Leu-Phe | - |
Vibrio proteolyticus | |
3.4.11.10 | 85 | - |
Leu-Tyr | - |
Vibrio proteolyticus | |
3.4.11.10 | 145 | - |
Leu-methyl ester | - |
Vibrio proteolyticus | |
3.4.11.10 | 220 | - |
Leu-amide | - |
Vibrio proteolyticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.11.1 | 8 | 8.5 | - |
Vibrio proteolyticus |
3.4.11.10 | 8 | - |
assay at | Vibrio proteolyticus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.4.11.1 | 8 | 8.5 | - |
Vibrio proteolyticus |
3.4.11.10 | 8 | 8.5 | - |
Vibrio proteolyticus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.10 | 0.0002 | - |
Leu-bromomethyl ketone | - |
Vibrio proteolyticus |