BRENDA - Enzyme Database

rac-Glycerol 1:2-cyclic phosphate 2-phosphodiesterase, a new soluble phosphodiesterase of mammalian tissues

Clarke, N.; Dawson, R.M.C.; Biochem. J. 173, 579-589 (1978)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.42
2-mercaptoethanol
stimulation
Sus scrofa
3.1.4.42
cysteine
stimulation
Sus scrofa
3.1.4.42
dithiothreitol
stimulation
Sus scrofa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.42
2'-AMP
9 mM, 75% inhibition
Sus scrofa
3.1.4.42
2-naphthyl phosphate
0.45 mM, 64% inhibition
Sus scrofa
3.1.4.42
3'-AMP
9 mM, 68% inhibition
Sus scrofa
3.1.4.42
4-nitrophenyl phosphate
5.5 mM, 90% inhibition
Sus scrofa
3.1.4.42
5'-AMP
9 mM, 76% inhibition
Sus scrofa
3.1.4.42
ATP
9 mM, 68% inhibition
Sus scrofa
3.1.4.42
cetyl phosphate
0.45 mM, 29% inhibition
Sus scrofa
3.1.4.42
dibutyryl glycerol phosphate
0.45 mM, 40% inhibition
Sus scrofa
3.1.4.42
ethyl phosphate
0.25 mM, 34% inhibition
Sus scrofa
3.1.4.42
glycerol 1-phosphate
5 mM, 16% inhibition
Sus scrofa
3.1.4.42
methyl phosphate
0.25 mM, 34% inhibition
Sus scrofa
3.1.4.42
Phenyl phosphate
0.45 mM, 68% inhibition
Sus scrofa
3.1.4.42
phosphatidic acid
0.45 mM, 43% inhibition
Sus scrofa
3.1.4.42
PO43-
-
Sus scrofa
3.1.4.42
pyridoxal 5'-phosphate
0.25 mM, 14% inhibition
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.42
7.2
-
glycerol 1,2-cyclic phosphate
-
Sus scrofa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.42
cytoplasm
-
Cavia porcellus
5737
-
3.1.4.42
cytoplasm
-
Mus musculus
5737
-
3.1.4.42
cytoplasm
-
Ovis aries
5737
-
3.1.4.42
cytoplasm
-
Rattus norvegicus
5737
-
3.1.4.42
cytoplasm
-
Sus scrofa
5737
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.42
Fe2+
stimulates
Sus scrofa
3.1.4.42
additional information
no requirement for Ca2+ and Mg2+
Sus scrofa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.42
35500
-
gel filtration
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.42
Cavia porcellus
-
-
-
3.1.4.42
Mus musculus
-
-
-
3.1.4.42
Ovis aries
-
-
-
3.1.4.42
Rattus norvegicus
-
-
-
3.1.4.42
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.42
-
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.42
cerebral hemisphere
low activity
Cavia porcellus
-
3.1.4.42
cerebral hemisphere
low activity
Rattus norvegicus
-
3.1.4.42
intestinal mucosa
-
Rattus norvegicus
-
3.1.4.42
kidney
-
Cavia porcellus
-
3.1.4.42
kidney
-
Mus musculus
-
3.1.4.42
kidney
-
Ovis aries
-
3.1.4.42
kidney
-
Sus scrofa
-
3.1.4.42
kidney
-
Rattus norvegicus
-
3.1.4.42
liver
-
Cavia porcellus
-
3.1.4.42
liver
-
Mus musculus
-
3.1.4.42
liver
-
Ovis aries
-
3.1.4.42
liver
-
Rattus norvegicus
-
3.1.4.42
liver
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.42
322
-
-
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.42
2',3'-cyclic AMP + H2O
-
81113
Sus scrofa
3'-AMP + 2'-AMP
69-72% 3'-AMP and 28-31% 2'-AMP
81113
Sus scrofa
?
3.1.4.42
3',5'-cyclic AMP + H2O
-
81113
Sus scrofa
5'-AMP
-
81113
Sus scrofa
?
3.1.4.42
3',5'-cyclic GMP + H2O
very low activity
81113
Sus scrofa
?
-
-
-
?
3.1.4.42
bis-(4-nitrophenyl) phosphate + H2O
very low activity
81113
Sus scrofa
?
-
-
-
?
3.1.4.42
butyrylglycerol cyclic phosphate + H2O
-
81113
Sus scrofa
butyrylglycerol phosphate
-
81113
Sus scrofa
?
3.1.4.42
glycerol 1,2-cyclic phosphate + H2O
racemic substrate
81113
Cavia porcellus
glycerol phosphate
-
81113
Cavia porcellus
?
3.1.4.42
glycerol 1,2-cyclic phosphate + H2O
racemic substrate
81113
Mus musculus
glycerol phosphate
-
81113
Mus musculus
?
3.1.4.42
glycerol 1,2-cyclic phosphate + H2O
racemic substrate
81113
Rattus norvegicus
glycerol phosphate
-
81113
Rattus norvegicus
?
3.1.4.42
glycerol 1,2-cyclic phosphate + H2O
racemic substrate
81113
Sus scrofa
glycerol phosphate
-
81113
Sus scrofa
?
3.1.4.42
glycerol 1,2-cyclic phosphate + H2O
racemic substrate
81113
Ovis aries
glycerol phosphate
-
81113
Ovis aries
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.4.42
38
-
assay at
Cavia porcellus
3.1.4.42
38
-
assay at
Mus musculus
3.1.4.42
38
-
assay at
Ovis aries
3.1.4.42
38
-
assay at
Rattus norvegicus
3.1.4.42
38
-
assay at
Sus scrofa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.42
6.9
7.5
-
Sus scrofa
3.1.4.42
6.9
-
beta,beta-dimethylglutarate buffer
Sus scrofa
3.1.4.42
7.4
-
imidazole /HCl buffer
Sus scrofa
3.1.4.42
7.5
-
glycylglycine buffer
Sus scrofa
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.42
2-mercaptoethanol
stimulation
Sus scrofa
3.1.4.42
cysteine
stimulation
Sus scrofa
3.1.4.42
dithiothreitol
stimulation
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.42
2'-AMP
9 mM, 75% inhibition
Sus scrofa
3.1.4.42
2-naphthyl phosphate
0.45 mM, 64% inhibition
Sus scrofa
3.1.4.42
3'-AMP
9 mM, 68% inhibition
Sus scrofa
3.1.4.42
4-nitrophenyl phosphate
5.5 mM, 90% inhibition
Sus scrofa
3.1.4.42
5'-AMP
9 mM, 76% inhibition
Sus scrofa
3.1.4.42
ATP
9 mM, 68% inhibition
Sus scrofa
3.1.4.42
cetyl phosphate
0.45 mM, 29% inhibition
Sus scrofa
3.1.4.42
dibutyryl glycerol phosphate
0.45 mM, 40% inhibition
Sus scrofa
3.1.4.42
ethyl phosphate
0.25 mM, 34% inhibition
Sus scrofa
3.1.4.42
glycerol 1-phosphate
5 mM, 16% inhibition
Sus scrofa
3.1.4.42
methyl phosphate
0.25 mM, 34% inhibition
Sus scrofa
3.1.4.42
Phenyl phosphate
0.45 mM, 68% inhibition
Sus scrofa
3.1.4.42
phosphatidic acid
0.45 mM, 43% inhibition
Sus scrofa
3.1.4.42
PO43-
-
Sus scrofa
3.1.4.42
pyridoxal 5'-phosphate
0.25 mM, 14% inhibition
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.42
7.2
-
glycerol 1,2-cyclic phosphate
-
Sus scrofa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.42
cytoplasm
-
Cavia porcellus
5737
-
3.1.4.42
cytoplasm
-
Mus musculus
5737
-
3.1.4.42
cytoplasm
-
Ovis aries
5737
-
3.1.4.42
cytoplasm
-
Rattus norvegicus
5737
-
3.1.4.42
cytoplasm
-
Sus scrofa
5737
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.42
Fe2+
stimulates
Sus scrofa
3.1.4.42
additional information
no requirement for Ca2+ and Mg2+
Sus scrofa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.42
35500
-
gel filtration
Sus scrofa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.42
-
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.42
cerebral hemisphere
low activity
Cavia porcellus
-
3.1.4.42
cerebral hemisphere
low activity
Rattus norvegicus
-
3.1.4.42
intestinal mucosa
-
Rattus norvegicus
-
3.1.4.42
kidney
-
Cavia porcellus
-
3.1.4.42
kidney
-
Mus musculus
-
3.1.4.42
kidney
-
Ovis aries
-
3.1.4.42
kidney
-
Sus scrofa
-
3.1.4.42
kidney
-
Rattus norvegicus
-
3.1.4.42
liver
-
Cavia porcellus
-
3.1.4.42
liver
-
Mus musculus
-
3.1.4.42
liver
-
Ovis aries
-
3.1.4.42
liver
-
Rattus norvegicus
-
3.1.4.42
liver
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.42
322
-
-
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.42
2',3'-cyclic AMP + H2O
-
81113
Sus scrofa
3'-AMP + 2'-AMP
69-72% 3'-AMP and 28-31% 2'-AMP
81113
Sus scrofa
?
3.1.4.42
3',5'-cyclic AMP + H2O
-
81113
Sus scrofa
5'-AMP
-
81113
Sus scrofa
?
3.1.4.42
3',5'-cyclic GMP + H2O
very low activity
81113
Sus scrofa
?
-
-
-
?
3.1.4.42
bis-(4-nitrophenyl) phosphate + H2O
very low activity
81113
Sus scrofa
?
-
-
-
?
3.1.4.42
butyrylglycerol cyclic phosphate + H2O
-
81113
Sus scrofa
butyrylglycerol phosphate
-
81113
Sus scrofa
?
3.1.4.42
glycerol 1,2-cyclic phosphate + H2O
racemic substrate
81113
Cavia porcellus
glycerol phosphate
-
81113
Cavia porcellus
?
3.1.4.42
glycerol 1,2-cyclic phosphate + H2O
racemic substrate
81113
Mus musculus
glycerol phosphate
-
81113
Mus musculus
?
3.1.4.42
glycerol 1,2-cyclic phosphate + H2O
racemic substrate
81113
Rattus norvegicus
glycerol phosphate
-
81113
Rattus norvegicus
?
3.1.4.42
glycerol 1,2-cyclic phosphate + H2O
racemic substrate
81113
Sus scrofa
glycerol phosphate
-
81113
Sus scrofa
?
3.1.4.42
glycerol 1,2-cyclic phosphate + H2O
racemic substrate
81113
Ovis aries
glycerol phosphate
-
81113
Ovis aries
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.4.42
38
-
assay at
Cavia porcellus
3.1.4.42
38
-
assay at
Mus musculus
3.1.4.42
38
-
assay at
Ovis aries
3.1.4.42
38
-
assay at
Rattus norvegicus
3.1.4.42
38
-
assay at
Sus scrofa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.42
6.9
7.5
-
Sus scrofa
3.1.4.42
6.9
-
beta,beta-dimethylglutarate buffer
Sus scrofa
3.1.4.42
7.4
-
imidazole /HCl buffer
Sus scrofa
3.1.4.42
7.5
-
glycylglycine buffer
Sus scrofa