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Literature summary extracted from

  • Clarke, N.; Dawson, R.M.C.
    rac-Glycerol 1:2-cyclic phosphate 2-phosphodiesterase, a new soluble phosphodiesterase of mammalian tissues (1978), Biochem. J., 173, 579-589.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.42 2-mercaptoethanol stimulation Sus scrofa
3.1.4.42 cysteine stimulation Sus scrofa
3.1.4.42 dithiothreitol stimulation Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.42 2'-AMP 9 mM, 75% inhibition Sus scrofa
3.1.4.42 2-naphthyl phosphate 0.45 mM, 64% inhibition Sus scrofa
3.1.4.42 3'-AMP 9 mM, 68% inhibition Sus scrofa
3.1.4.42 4-nitrophenyl phosphate 5.5 mM, 90% inhibition Sus scrofa
3.1.4.42 5'-AMP 9 mM, 76% inhibition Sus scrofa
3.1.4.42 ATP 9 mM, 68% inhibition Sus scrofa
3.1.4.42 cetyl phosphate 0.45 mM, 29% inhibition Sus scrofa
3.1.4.42 dibutyryl glycerol phosphate 0.45 mM, 40% inhibition Sus scrofa
3.1.4.42 ethyl phosphate 0.25 mM, 34% inhibition Sus scrofa
3.1.4.42 glycerol 1-phosphate 5 mM, 16% inhibition Sus scrofa
3.1.4.42 methyl phosphate 0.25 mM, 34% inhibition Sus scrofa
3.1.4.42 Phenyl phosphate 0.45 mM, 68% inhibition Sus scrofa
3.1.4.42 phosphatidic acid 0.45 mM, 43% inhibition Sus scrofa
3.1.4.42 PO43-
-
 Sus scrofa
3.1.4.42 pyridoxal 5'-phosphate 0.25 mM, 14% inhibition Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.42 7.2
-
 glycerol 1,2-cyclic phosphate
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.42 cytoplasm
-
 Cavia porcellus 5737
-
3.1.4.42 cytoplasm
-
 Mus musculus 5737
-
3.1.4.42 cytoplasm
-
 Rattus norvegicus 5737
-
3.1.4.42 cytoplasm
-
 Sus scrofa 5737
-
3.1.4.42 cytoplasm
-
 Ovis aries 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.42 Fe2+ stimulates Sus scrofa
3.1.4.42 additional information no requirement for Ca2+ and Mg2+ Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.42 35500
-
 gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.42 Cavia porcellus
-
-
-
3.1.4.42 Mus musculus
-
-
-
3.1.4.42 Ovis aries
-
-
-
3.1.4.42 Rattus norvegicus
-
-
-
3.1.4.42 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.42
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.42 cerebral hemisphere low activity Cavia porcellus
-
3.1.4.42 cerebral hemisphere low activity Rattus norvegicus
-
3.1.4.42 intestinal mucosa
-
 Rattus norvegicus
-
3.1.4.42 kidney
-
 Cavia porcellus
-
3.1.4.42 kidney
-
 Mus musculus
-
3.1.4.42 kidney
-
 Rattus norvegicus
-
3.1.4.42 kidney
-
 Sus scrofa
-
3.1.4.42 kidney
-
 Ovis aries
-
3.1.4.42 liver
-
 Cavia porcellus
-
3.1.4.42 liver
-
 Mus musculus
-
3.1.4.42 liver
-
 Rattus norvegicus
-
3.1.4.42 liver
-
 Sus scrofa
-
3.1.4.42 liver
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.42 322
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.42 2',3'-cyclic AMP + H2O
-
 Sus scrofa 3'-AMP + 2'-AMP 69-72% 3'-AMP and 28-31% 2'-AMP ?
3.1.4.42 3',5'-cyclic AMP + H2O
-
 Sus scrofa 5'-AMP
-
 ?
3.1.4.42 3',5'-cyclic GMP + H2O very low activity Sus scrofa ?
-
 ?
3.1.4.42 bis-(4-nitrophenyl) phosphate + H2O very low activity Sus scrofa ?
-
 ?
3.1.4.42 butyrylglycerol cyclic phosphate + H2O
-
 Sus scrofa butyrylglycerol phosphate
-
 ?
3.1.4.42 glycerol 1,2-cyclic phosphate + H2O racemic substrate Cavia porcellus glycerol phosphate
-
 ?
3.1.4.42 glycerol 1,2-cyclic phosphate + H2O racemic substrate Mus musculus glycerol phosphate
-
 ?
3.1.4.42 glycerol 1,2-cyclic phosphate + H2O racemic substrate Rattus norvegicus glycerol phosphate
-
 ?
3.1.4.42 glycerol 1,2-cyclic phosphate + H2O racemic substrate Sus scrofa glycerol phosphate
-
 ?
3.1.4.42 glycerol 1,2-cyclic phosphate + H2O racemic substrate Ovis aries glycerol phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.42 38
-
 assay at Cavia porcellus
3.1.4.42 38
-
 assay at Mus musculus
3.1.4.42 38
-
 assay at Rattus norvegicus
3.1.4.42 38
-
 assay at Sus scrofa
3.1.4.42 38
-
 assay at Ovis aries

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.42 6.9
-
 beta,beta-dimethylglutarate buffer Sus scrofa
3.1.4.42 6.9 7.5
-
 Sus scrofa
3.1.4.42 7.4
-
 imidazole /HCl buffer Sus scrofa
3.1.4.42 7.5
-
 glycylglycine buffer Sus scrofa