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Literature summary extracted from

  • Lik-Shing Tsang, M.; Schiff, J.A.
    Properties of enzyme fraction A from Chlorella and copurification of 3 (2 ), 5-biphosphonucleoside 3 (2 )-phosphohydrolase, adenosine 5 phosphosulfate sulfohydrolase and adenosine-5-phosphosulfate cyclase activities (1976), Eur. J. Biochem., 65, 113-121.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.7 5'-AMP
-
 Auxenochlorella pyrenoidosa
3.1.3.7 adenosine 5'-phosphosulfate slight Auxenochlorella pyrenoidosa
3.1.3.7 adenosine-3',5'-bisphosphate inhibition at high concentration Auxenochlorella pyrenoidosa
3.1.3.7 Ca2+ in presence of Mg2+ Auxenochlorella pyrenoidosa
3.1.3.7 dithiothreitol slight Auxenochlorella pyrenoidosa
3.1.3.7 Na+ slight Auxenochlorella pyrenoidosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.7 0.2
-
 Guanosine 2',5'-bisphosphate
-
 Auxenochlorella pyrenoidosa
3.1.3.7 0.24
-
 adenosine 3',5'-bisphosphate
-
 Auxenochlorella pyrenoidosa
3.1.3.7 0.25
-
 adenosine 3'-phosphate 5'-phosphosulfate
-
 Auxenochlorella pyrenoidosa
3.1.3.7 0.25
-
 guanosine-3',5'-bisphosphate
-
 Auxenochlorella pyrenoidosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.7 Ca2+ activates slightly Auxenochlorella pyrenoidosa
3.1.3.7 Mg2+ activates Auxenochlorella pyrenoidosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.7 Auxenochlorella pyrenoidosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.7 partial, copurification with other enzymes Auxenochlorella pyrenoidosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.7 adenosine 2',5'-bisphosphate + H2O
-
 Auxenochlorella pyrenoidosa adenosine 5'-phosphate + phosphate
-
 ?
3.1.3.7 adenosine 3',5'-bisphosphate + H2O
-
 Auxenochlorella pyrenoidosa adenosine 5'-phosphate + phosphate
-
 ?
3.1.3.7 adenosine 3'-phosphate 5'-phosphosulfate + H2O
-
 Auxenochlorella pyrenoidosa adenosine 5'-phosphosulfate + phosphate
-
 ?
3.1.3.7 CoA + H2O
-
 Auxenochlorella pyrenoidosa ?
-
 ?
3.1.3.7 guanosine 2',5'-bisphosphate + H2O
-
 Auxenochlorella pyrenoidosa guanosine 2'-phosphate + phosphate
-
 ?
3.1.3.7 guanosine 3',5'-bisphosphate + H2O
-
 Auxenochlorella pyrenoidosa guanosine 5'-phosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.7 30
-
 assay at Auxenochlorella pyrenoidosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.7 9
-
 assay at Auxenochlorella pyrenoidosa