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Literature summary extracted from
- Deoxythymidylate 5-nucleotidase. Purification and properties of an enzyme found after infection of Bacillus subtilis with phage SP5C (1966), J. Biol. Chem., 241, 5095-5101.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.35 | chloramphenicol | inhibits the synthesis of the enzyme | Bacillus subtilis |  |
| 3.1.3.35 | EDTA | - |
Bacillus subtilis | |
| 3.1.3.35 | F- | - |
Bacillus subtilis | |
| 3.1.3.35 | Mn2+ | inhibition above 0.4 mM | Bacillus subtilis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.35 | 0.7 | - |
dGMP | - |
Bacillus subtilis | |
| 3.1.3.35 | 1 | - |
dTMP | - |
Bacillus subtilis | |
| 3.1.3.35 | 6 | - |
deoxy-5-methyl-CMP | - |
Bacillus subtilis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.35 | Mg2+ | activation, optimal concentration 8 mM | Bacillus subtilis | |
| 3.1.3.35 | Mn2+ | activation, optimal concentration 0.4 mM, inhibition above | Bacillus subtilis | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.35 | Bacillus subtilis | - |
SB19, infected with phage SP5C | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.35 | partial | Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.35 | deoxy-5-methyl-CMP + phosphate | - |
Bacillus subtilis | deoxy-5-methylcytidine + phosphate | - |
? | |
| 3.1.3.35 | deoxythymidine 5'-phosphate + H2O | - |
Bacillus subtilis | deoxythymidine + phosphate | - |
? | |
| 3.1.3.35 | dGMP + H2O | - |
Bacillus subtilis | deoxyguanosine + phosphate | - |
? | |
| 3.1.3.35 | dTMP + H2O | - |
Bacillus subtilis | thymidine + phosphate | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.35 | 6.9 | - |
- |
Bacillus subtilis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.35 | 6.4 | 7.8 | 80% of maximal activity at pH 6.4, 42% of maximal activity at pH 7.8, no activity at pH 6.0 | Bacillus subtilis |
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