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Literature summary extracted from

  • Habraken, Y.; Verly, W.G.
    Further purification and characterization of the DNA 3-phosphatase from rat-liver chromatin which is also a polynucleotide 5-hydroxyl kinase (1988), Eur. J. Biochem., 171, 59-66.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.78 ATP protects against heat denaturation and tryptic digestion Rattus norvegicus
3.1.3.32 ATP protects against thermal denaturation and trypsin digestion Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.78 diphosphate
-
 Rattus norvegicus
2.7.1.78 EDTA
-
 Rattus norvegicus
2.7.1.78 p-hydroxymercuribenzoate 2-mercaptoethanol prevents inhibition Rattus norvegicus
2.7.1.78 phosphate
-
 Rattus norvegicus
2.7.1.78 sulfate
-
 Rattus norvegicus
3.1.3.32 diphosphate no effect on 3'-phosphatase, inhibition of 5'-hydroxyl kinase Rattus norvegicus
3.1.3.32 EDTA
-
 Rattus norvegicus
3.1.3.32 PO43- 5'-hydroxyl kinase, little effect on 3'-phosphatase Rattus norvegicus
3.1.3.32 SO42- 5'-hydroxyl kinase, little effect on 3'-phosphatase Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.78 0.00069
-
 ATP pH 5.5, 37°C Rattus norvegicus
2.7.1.78 0.0039
-
 5'-OH-DNA pH 5.5, 37°C Rattus norvegicus
3.1.3.32 0.0039
-
 DNA with 5'-hydroxyl ends
-
 Rattus norvegicus
3.1.3.32 0.069
-
 ATP
-
 Rattus norvegicus
3.1.3.32 0.548
-
 3'-phosphopolynucleotide
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.78 chromatin
-
 Rattus norvegicus 785
-
3.1.3.32 chromatin
-
 Rattus norvegicus 785
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.78 divalent cations absolutely required Rattus norvegicus
2.7.1.78 Mg2+ stimulates Rattus norvegicus
2.7.1.78 Mn2+ most effective divalent cation in activation, best at 1 mM Rattus norvegicus
2.7.1.78 additional information maximal activity at ionic strength of 135 mM Rattus norvegicus
2.7.1.78 Zn2+ less effective Rattus norvegicus
3.1.3.32 Mg2+ stimulates Rattus norvegicus
3.1.3.32 Mn2+ stimulates Rattus norvegicus
3.1.3.32 Zn2+ stimulates less efficiently than Mg2+, Mn2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.78 79000
-
 gel filtration Rattus norvegicus
3.1.3.32 40000
-
 2 * 40000, SDS-PAGE Rattus norvegicus
3.1.3.32 79000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA Rattus norvegicus
-
 ADP + 5'-phospho-DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Rattus norvegicus
-
 enzyme also has DNA 3'-phosphatase activity
-
3.1.3.32 Rattus norvegicus
-
 enzyme also is polynucleotide 5'-hydroxyl kinase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.78 39fold Rattus norvegicus
3.1.3.32 enzyme also is polynucleotide 5'-hydroxyl kinase Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.32 a 3'-phosphopolynucleotide + H2O = a polynucleotide + phosphate also 5-hydroxylkinase activity Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.78 liver
-
 Rattus norvegicus
-
3.1.3.32 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.78 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA
-
 Rattus norvegicus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA native and heat denatured DNA Rattus norvegicus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + nucleoside-3'-monophosphate
-
 Rattus norvegicus ADP + nucleoside-3',5'-diphosphate
-
 ?
2.7.1.78 additional information enzyme also has DNA 3'-phosphatase activity Rattus norvegicus ?
-
 ?
3.1.3.32 3'-phosphopolynucleotide + H2O
-
 Rattus norvegicus polynucleotide + phosphate
-
 ?
3.1.3.32 DNA with 3'-phosphate ends + H2O
-
 Rattus norvegicus DNA + phosphate
-
 ?
3.1.3.32 DNA with 5'-hydroxyl ends + ATP
-
 Rattus norvegicus DNA with 5'-phosphate ends + ADP
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.32 dimer 2 * 40000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.78 More enzyme also possesses a 3'-phosphatase activity Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.78 37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.32 37
-
 5'-hydroxyl kinase activity inactivated much faster than 3'-phosphatase activity, ATP protects against thermal denaturation Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.78 additional information
-
 pH-optimum of 3'-phosphatase activity is 6.0 Rattus norvegicus
2.7.1.78 5.5
-
 assay at Rattus norvegicus
3.1.3.32 5.5
-
 5'-hydroxyl kinase activity Rattus norvegicus
3.1.3.32 6
-
 3'-phosphatase activity, 2 optima: pH 6.0 and pH 7.5 Rattus norvegicus
3.1.3.32 7.5
-
 3'-phosphatase activity, 2 optima: pH 6.0 and pH 7.5 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.78 ATP dependent on Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.78 Rattus norvegicus
-
-
 8.6