BRENDA - Enzyme Database show

Properties of a non-specific nucleotidase in the membrane of rabbit red cells

Delaunay, J.; Fischer, S.; Piau, J.P.; Tortolero, M.; Schapira, G.; Biochim. Biophys. Acta 527, 425-431 (1978)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.31
Cu2+
-
Oryctolagus cuniculus
3.1.3.31
Hg2+
-
Oryctolagus cuniculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.31
1
-
5'-dAMP
-
Oryctolagus cuniculus
3.1.3.31
13
-
5'-AMP
-
Oryctolagus cuniculus
3.1.3.31
14
-
5'-dUMP
-
Oryctolagus cuniculus
3.1.3.31
42
-
3'-AMP
-
Oryctolagus cuniculus
3.1.3.31
95
-
5'-UMP
-
Oryctolagus cuniculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.31
plasma membrane
-
Oryctolagus cuniculus
5886
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.31
K+
0-50 mM activate
Oryctolagus cuniculus
3.1.3.31
Mg2+
0-10 mM activate
Oryctolagus cuniculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.31
Oryctolagus cuniculus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.31
0.004
-
5'-dAMP
Oryctolagus cuniculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.31
2'-AMP + H2O
-
81064
Oryctolagus cuniculus
adenosine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
2'-GMP + H2O
-
81064
Oryctolagus cuniculus
?
-
-
-
?
3.1.3.31
2'-UMP + H2O
-
81064
Oryctolagus cuniculus
deoxyuridine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
3'-AMP + H2O
-
81064
Oryctolagus cuniculus
adenosine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
3'-dCMP + H2O
i.e. 2'-deoxycytidine 3'-phosphate
81064
Oryctolagus cuniculus
deoxycytidine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
3'-GMP + H2O
-
81064
Oryctolagus cuniculus
guanosine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
3'-UMP + H2O
-
81064
Oryctolagus cuniculus
uridine + phosphate
-
-
-
?
3.1.3.31
5'-AMP + H2O
-
81064
Oryctolagus cuniculus
adenosine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
5'-CMP + H2O
-
81064
Oryctolagus cuniculus
cytidine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
5'-dAMP + H2O
i.e. 2'-deoxyadenosine 5'-phosphate
81064
Oryctolagus cuniculus
deoxyadenosine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
5'-dGMP + H2O
i.e. 2'-deoxyguanosine 5'-phosphate
81064
Oryctolagus cuniculus
deoxyguanosine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
5'-dUMP + H2O
i.e. 2'-deoxyuridine 5'-phosphate
81064
Oryctolagus cuniculus
deoxyuridine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
5'-GMP + H2O
-
81064
Oryctolagus cuniculus
guanosine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
5'-IMP + H2O
-
81064
Oryctolagus cuniculus
inosine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
5'-UMP + H2O
-
81064
Oryctolagus cuniculus
uridine + phosphate
-
81064
Oryctolagus cuniculus
?
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.1.3.31
25
41
activation energy in this range: 12.4 kcal/mol
Oryctolagus cuniculus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.31
51
-
inactivation above
Oryctolagus cuniculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.31
Cu2+
-
Oryctolagus cuniculus
3.1.3.31
Hg2+
-
Oryctolagus cuniculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.31
1
-
5'-dAMP
-
Oryctolagus cuniculus
3.1.3.31
13
-
5'-AMP
-
Oryctolagus cuniculus
3.1.3.31
14
-
5'-dUMP
-
Oryctolagus cuniculus
3.1.3.31
42
-
3'-AMP
-
Oryctolagus cuniculus
3.1.3.31
95
-
5'-UMP
-
Oryctolagus cuniculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.31
plasma membrane
-
Oryctolagus cuniculus
5886
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.31
K+
0-50 mM activate
Oryctolagus cuniculus
3.1.3.31
Mg2+
0-10 mM activate
Oryctolagus cuniculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.31
0.004
-
5'-dAMP
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.31
2'-AMP + H2O
-
81064
Oryctolagus cuniculus
adenosine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
2'-GMP + H2O
-
81064
Oryctolagus cuniculus
?
-
-
-
?
3.1.3.31
2'-UMP + H2O
-
81064
Oryctolagus cuniculus
deoxyuridine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
3'-AMP + H2O
-
81064
Oryctolagus cuniculus
adenosine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
3'-dCMP + H2O
i.e. 2'-deoxycytidine 3'-phosphate
81064
Oryctolagus cuniculus
deoxycytidine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
3'-GMP + H2O
-
81064
Oryctolagus cuniculus
guanosine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
3'-UMP + H2O
-
81064
Oryctolagus cuniculus
uridine + phosphate
-
-
-
?
3.1.3.31
5'-AMP + H2O
-
81064
Oryctolagus cuniculus
adenosine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
5'-CMP + H2O
-
81064
Oryctolagus cuniculus
cytidine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
5'-dAMP + H2O
i.e. 2'-deoxyadenosine 5'-phosphate
81064
Oryctolagus cuniculus
deoxyadenosine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
5'-dGMP + H2O
i.e. 2'-deoxyguanosine 5'-phosphate
81064
Oryctolagus cuniculus
deoxyguanosine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
5'-dUMP + H2O
i.e. 2'-deoxyuridine 5'-phosphate
81064
Oryctolagus cuniculus
deoxyuridine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
5'-GMP + H2O
-
81064
Oryctolagus cuniculus
guanosine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
5'-IMP + H2O
-
81064
Oryctolagus cuniculus
inosine + phosphate
-
81064
Oryctolagus cuniculus
?
3.1.3.31
5'-UMP + H2O
-
81064
Oryctolagus cuniculus
uridine + phosphate
-
81064
Oryctolagus cuniculus
?
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.1.3.31
25
41
activation energy in this range: 12.4 kcal/mol
Oryctolagus cuniculus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.31
51
-
inactivation above
Oryctolagus cuniculus