BRENDA - Enzyme Database show

Rat spleen cytoplasmic nucleotidase: characterization and its physiological significance

Hokari, S.; Sakagishi, Y.; Int. J. Biochem. 20, 1405-1410 (1988)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.31
0.27
-
3'-UMP
-
Rattus norvegicus
3.1.3.31
0.38
-
3'-dTMP
-
Rattus norvegicus
3.1.3.31
0.38
-
3'-dUMP
-
Rattus norvegicus
3.1.3.31
0.78
-
5'-dTMP
-
Rattus norvegicus
3.1.3.31
1.05
-
5'-UMP
-
Rattus norvegicus
3.1.3.31
1.35
-
5'-dUMP
-
Rattus norvegicus
3.1.3.31
3.07
-
5'-dCMP
-
Rattus norvegicus
3.1.3.31
3.17
-
3'-AMP
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.31
cytoplasm
-
Rattus norvegicus
5737
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.31
45000
50000
spleen, cytoplasm, gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.31
additional information
Rattus norvegicus
physiological significance for spleen cytoplasm in the reutilization of nucleotides, especially for thymidine and uridine nucleotides
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.31
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.31
spleen, cytoplasm
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.31
kidney
-
Rattus norvegicus
-
3.1.3.31
liver
-
Rattus norvegicus
-
3.1.3.31
small intestine
-
Rattus norvegicus
-
3.1.3.31
spleen
-
Rattus norvegicus
-
3.1.3.31
testis
-
Rattus norvegicus
-
3.1.3.31
thymus
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.31
3'-AMP + H2O
3.2% of the activity with 5'-dUMP
81058
Rattus norvegicus
adenosine + phosphate
-
-
-
-
3.1.3.31
3'-CMP + H2O
2% of the activity with 5'-dUMP
81058
Rattus norvegicus
cytidine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
3'-dAMP + H2O
9.1% of the activity with 5'-dUMP
81058
Rattus norvegicus
3'-deoxyadenosine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
3'-dAMP + H2O
i.e. 2'-deoxyadenosine 3'-phosphate
81058
Rattus norvegicus
3'-deoxyadenosine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
3'-dCMP + H2O
6.5% of the activity with 5'-dUMP
81058
Rattus norvegicus
deoxycytidine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
3'-dCMP + H2O
i.e. 2'-deoxycytidine 3'-phosphate
81058
Rattus norvegicus
deoxycytidine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
3'-dTMP + H2O
113.7% of the activity with 5'-dUMP
81058
Rattus norvegicus
deoxythymidine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
3'-dTMP + H2O
i.e. 2'-deoxythymidine 3'-phosphate
81058
Rattus norvegicus
deoxythymidine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
3'-dUMP + H2O
i.e. 2'-deoxyuridine 3'-phosphate
81058
Rattus norvegicus
deoxyuridine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
3'-dUMP + H2O
153.5% of the activity with 5'-dUMP
81058
Rattus norvegicus
deoxyuridine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
3'-UMP + H2O
122.8% of the activity with 5'-dUMP
81058
Rattus norvegicus
uridine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
5'-dCMP + H2O
7.8% of the activity with 5'-dUMP
81058
Rattus norvegicus
deoxycytidine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
5'-dCMP + H2O
i.e. 2'-deoxycytidine 5'-phosphate
81058
Rattus norvegicus
deoxycytidine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
5'-dTMP + H2O
77.7% of the activity with 5'-dUMP
81058
Rattus norvegicus
deoxythymidine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
5'-dTMP + H2O
i.e. 2'-deoxythymidine 5'-phosphate
81058
Rattus norvegicus
deoxythymidine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
5'-dUMP + H2O
i.e. 2'-deoxyuridine 5'-phosphate
81058
Rattus norvegicus
deoxyuridine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
5'-IMP + H2O
13% of the activity with 5'-dUMP
81058
Rattus norvegicus
inosine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
5'-UMP + H2O
8.2% of the activity with 5'-dUMP
81058
Rattus norvegicus
uridine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
additional information
physiological significance for spleen cytoplasm in the reutilization of nucleotides, especially for thymidine and uridine nucleotides
81058
Rattus norvegicus
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.31
6
-
substrates 3'-UMP, 5'-UMP, 5'-dTMP, 3'-dCMP, 3'-CMP, 5'-dCMP, 3'-dAMP, 3'-AMP, 5'-IMP
Rattus norvegicus
3.1.3.31
6.5
-
substrates 3'-dTMP or 3'-dUMP
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.31
0.27
-
3'-UMP
-
Rattus norvegicus
3.1.3.31
0.38
-
3'-dTMP
-
Rattus norvegicus
3.1.3.31
0.38
-
3'-dUMP
-
Rattus norvegicus
3.1.3.31
0.78
-
5'-dTMP
-
Rattus norvegicus
3.1.3.31
1.05
-
5'-UMP
-
Rattus norvegicus
3.1.3.31
1.35
-
5'-dUMP
-
Rattus norvegicus
3.1.3.31
3.07
-
5'-dCMP
-
Rattus norvegicus
3.1.3.31
3.17
-
3'-AMP
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.31
cytoplasm
-
Rattus norvegicus
5737
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.31
45000
50000
spleen, cytoplasm, gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.31
additional information
Rattus norvegicus
physiological significance for spleen cytoplasm in the reutilization of nucleotides, especially for thymidine and uridine nucleotides
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.31
spleen, cytoplasm
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.31
kidney
-
Rattus norvegicus
-
3.1.3.31
liver
-
Rattus norvegicus
-
3.1.3.31
small intestine
-
Rattus norvegicus
-
3.1.3.31
spleen
-
Rattus norvegicus
-
3.1.3.31
testis
-
Rattus norvegicus
-
3.1.3.31
thymus
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.31
3'-AMP + H2O
3.2% of the activity with 5'-dUMP
81058
Rattus norvegicus
adenosine + phosphate
-
-
-
-
3.1.3.31
3'-CMP + H2O
2% of the activity with 5'-dUMP
81058
Rattus norvegicus
cytidine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
3'-dAMP + H2O
9.1% of the activity with 5'-dUMP
81058
Rattus norvegicus
3'-deoxyadenosine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
3'-dAMP + H2O
i.e. 2'-deoxyadenosine 3'-phosphate
81058
Rattus norvegicus
3'-deoxyadenosine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
3'-dCMP + H2O
6.5% of the activity with 5'-dUMP
81058
Rattus norvegicus
deoxycytidine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
3'-dCMP + H2O
i.e. 2'-deoxycytidine 3'-phosphate
81058
Rattus norvegicus
deoxycytidine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
3'-dTMP + H2O
113.7% of the activity with 5'-dUMP
81058
Rattus norvegicus
deoxythymidine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
3'-dTMP + H2O
i.e. 2'-deoxythymidine 3'-phosphate
81058
Rattus norvegicus
deoxythymidine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
3'-dUMP + H2O
i.e. 2'-deoxyuridine 3'-phosphate
81058
Rattus norvegicus
deoxyuridine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
3'-dUMP + H2O
153.5% of the activity with 5'-dUMP
81058
Rattus norvegicus
deoxyuridine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
3'-UMP + H2O
122.8% of the activity with 5'-dUMP
81058
Rattus norvegicus
uridine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
5'-dCMP + H2O
7.8% of the activity with 5'-dUMP
81058
Rattus norvegicus
deoxycytidine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
5'-dCMP + H2O
i.e. 2'-deoxycytidine 5'-phosphate
81058
Rattus norvegicus
deoxycytidine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
5'-dTMP + H2O
77.7% of the activity with 5'-dUMP
81058
Rattus norvegicus
deoxythymidine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
5'-dTMP + H2O
i.e. 2'-deoxythymidine 5'-phosphate
81058
Rattus norvegicus
deoxythymidine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
5'-dUMP + H2O
i.e. 2'-deoxyuridine 5'-phosphate
81058
Rattus norvegicus
deoxyuridine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
5'-IMP + H2O
13% of the activity with 5'-dUMP
81058
Rattus norvegicus
inosine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
5'-UMP + H2O
8.2% of the activity with 5'-dUMP
81058
Rattus norvegicus
uridine + phosphate
-
81058
Rattus norvegicus
?
3.1.3.31
additional information
physiological significance for spleen cytoplasm in the reutilization of nucleotides, especially for thymidine and uridine nucleotides
81058
Rattus norvegicus
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.31
6
-
substrates 3'-UMP, 5'-UMP, 5'-dTMP, 3'-dCMP, 3'-CMP, 5'-dCMP, 3'-dAMP, 3'-AMP, 5'-IMP
Rattus norvegicus
3.1.3.31
6.5
-
substrates 3'-dTMP or 3'-dUMP
Rattus norvegicus