BRENDA - Enzyme Database

Purification and characterization of S-formylglutathione hydrolase from human, rat and fish tissues

Koivusalo, M.; Lapatto, R.; Uotila, L.; Adv. Exp. Med. Biol. 372, 427-434 (1995)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.12
0.019
-
4-yethylumbelliferyl acetate
-
Homo sapiens
3.1.2.12
0.2
-
S-formylglutathione
-
Homo sapiens
3.1.2.12
0.49
-
p-nitrophenyl acetate
-
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.12
32000
-
2 * 32000, SDS-PAGE
Homo sapiens
3.1.2.12
53000
-
gel filtration
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.2.12
Homo sapiens
-
-
-
3.1.2.12
Lota maculosa
-
burbot
-
3.1.2.12
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.2.12
-
Homo sapiens
3.1.2.12
partial
Lota maculosa
3.1.2.12
partial
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.12
erythrocyte
-
Homo sapiens
-
3.1.2.12
liver
-
Homo sapiens
-
3.1.2.12
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.12
26.9
-
-
Lota maculosa
3.1.2.12
208
-
-
Rattus norvegicus
3.1.2.12
3390
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.12
4-methylumbelliferyl acetate + H2O
very low activity
80993
Rattus norvegicus
4-methylumbelliferone + acetate
-
80993
Rattus norvegicus
?
3.1.2.12
4-methylumbelliferyl acetate + H2O
very low activity
80993
Lota maculosa
4-methylumbelliferone + acetate
-
80993
Lota maculosa
?
3.1.2.12
p-nitrophenyl acetate + H2O
very low activity
80993
Rattus norvegicus
p-nitrophenol + acetate
-
80993
Rattus norvegicus
?
3.1.2.12
p-nitrophenyl acetate + H2O
very low activity
80993
Lota maculosa
p-nitrophenol + acetate
-
80993
Lota maculosa
?
3.1.2.12
S-acetylglutathione + H2O
-
80993
Homo sapiens
glutathione + acetate
-
80993
Homo sapiens
-
3.1.2.12
S-acetylglutathione + H2O
-
80993
Rattus norvegicus
glutathione + acetate
-
80993
Rattus norvegicus
?
3.1.2.12
S-acetylglutathione + H2O
-
80993
Lota maculosa
glutathione + acetate
-
80993
Lota maculosa
?
3.1.2.12
S-formylglutathione + H2O
-
80993
Homo sapiens
glutathione + formate
-
80993
Homo sapiens
-
3.1.2.12
S-formylglutathione + H2O
-
80993
Rattus norvegicus
glutathione + formate
-
80993
Rattus norvegicus
?
3.1.2.12
S-formylglutathione + H2O
-
80993
Lota maculosa
glutathione + formate
-
80993
Lota maculosa
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.2.12
dimer
2 * 32000, SDS-PAGE
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.12
0.019
-
4-yethylumbelliferyl acetate
-
Homo sapiens
3.1.2.12
0.2
-
S-formylglutathione
-
Homo sapiens
3.1.2.12
0.49
-
p-nitrophenyl acetate
-
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.12
32000
-
2 * 32000, SDS-PAGE
Homo sapiens
3.1.2.12
53000
-
gel filtration
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.12
-
Homo sapiens
3.1.2.12
partial
Lota maculosa
3.1.2.12
partial
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.12
erythrocyte
-
Homo sapiens
-
3.1.2.12
liver
-
Homo sapiens
-
3.1.2.12
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.12
26.9
-
-
Lota maculosa
3.1.2.12
208
-
-
Rattus norvegicus
3.1.2.12
3390
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.12
4-methylumbelliferyl acetate + H2O
very low activity
80993
Rattus norvegicus
4-methylumbelliferone + acetate
-
80993
Rattus norvegicus
?
3.1.2.12
4-methylumbelliferyl acetate + H2O
very low activity
80993
Lota maculosa
4-methylumbelliferone + acetate
-
80993
Lota maculosa
?
3.1.2.12
p-nitrophenyl acetate + H2O
very low activity
80993
Rattus norvegicus
p-nitrophenol + acetate
-
80993
Rattus norvegicus
?
3.1.2.12
p-nitrophenyl acetate + H2O
very low activity
80993
Lota maculosa
p-nitrophenol + acetate
-
80993
Lota maculosa
?
3.1.2.12
S-acetylglutathione + H2O
-
80993
Homo sapiens
glutathione + acetate
-
80993
Homo sapiens
-
3.1.2.12
S-acetylglutathione + H2O
-
80993
Rattus norvegicus
glutathione + acetate
-
80993
Rattus norvegicus
?
3.1.2.12
S-acetylglutathione + H2O
-
80993
Lota maculosa
glutathione + acetate
-
80993
Lota maculosa
?
3.1.2.12
S-formylglutathione + H2O
-
80993
Homo sapiens
glutathione + formate
-
80993
Homo sapiens
-
3.1.2.12
S-formylglutathione + H2O
-
80993
Rattus norvegicus
glutathione + formate
-
80993
Rattus norvegicus
?
3.1.2.12
S-formylglutathione + H2O
-
80993
Lota maculosa
glutathione + formate
-
80993
Lota maculosa
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.2.12
dimer
2 * 32000, SDS-PAGE
Homo sapiens