BRENDA - Enzyme Database

Oxidation of methanol by the yeast, Pichia pastoris. Purification and properties of the formaldehyde dehydrogenase

Allais, J.J.; Louktibi, A.; Baratti, J.; Agric. Biol. Chem. 47, 1509-1516 (1983)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
Cd2+
1 mM, complete inhibition
Candida boidinii
1.1.1.284
Cd2+
1 mM, complete inhibition
Komagataella pastoris
1.1.1.284
citrate
1 mM, 24% inhibition
Komagataella pastoris
1.1.1.284
CN-
-
Candida boidinii
1.1.1.284
CN-
1 mM, 48% inhibition
Komagataella pastoris
1.1.1.284
Cu2+
-
Candida boidinii
1.1.1.284
Cu2+
1 mM, 76% inhibition
Komagataella pastoris
1.1.1.284
EDTA
1 mM, 12% inhibition
Komagataella pastoris
1.1.1.284
Hg2+
-
Candida boidinii
1.1.1.284
Hg2+
1 mM, 90% inhibition
Komagataella pastoris
1.1.1.284
NADH
-
Candida boidinii
1.1.1.284
NADH
product inhibition
Komagataella pastoris
1.1.1.284
NO2-
1 mM, 14% inhibition
Komagataella pastoris
1.1.1.284
Zn2+
-
Candida boidinii
1.1.1.284
Zn2+
1 mM, 28% inhibition
Komagataella pastoris
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.24
-
NAD+
-
Komagataella pastoris
1.1.1.284
0.43
-
formaldehyde
-
Komagataella pastoris
1.1.1.284
0.48
-
glutathione
-
Komagataella pastoris
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.284
39000
-
2 * 39000, SDS-PAGE
Komagataella pastoris
1.1.1.284
84000
-
gel filtration
Komagataella pastoris
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.284
Candida boidinii
-
-
-
1.1.1.284
Kloeckera sp.
-
-
-
1.1.1.284
Komagataella pastoris
-
-
-
3.1.2.12
Komagataella pastoris
-
IFP 206
-
3.1.2.12
Komagataella pastoris IFP 206
-
IFP 206
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.284
-
Komagataella pastoris
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
10.2
-
-
Komagataella pastoris
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.284
4°C, in presence of glycerol, 70-80% recovery of activity after 7 days
Komagataella pastoris
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
-
80989
Komagataella pastoris
S-formylglutathione + NADH + H+
-
80989
Komagataella pastoris
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
80989
Candida boidinii
S-formylglutathione + NADH + H+
-
80989
Candida boidinii
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
80989
Kloeckera sp.
S-formylglutathione + NADH + H+
-
80989
Kloeckera sp.
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
80989
Komagataella pastoris IFP 206
S-formylglutathione + NADH + H+
-
80989
Komagataella pastoris IFP 206
?
3.1.2.12
S-formylglutathione + H2O
-
80989
Komagataella pastoris
glutathione + formate
-
80989
Komagataella pastoris
?
3.1.2.12
S-formylglutathione + H2O
-
80989
Komagataella pastoris IFP 206
glutathione + formate
-
80989
Komagataella pastoris IFP 206
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.284
dimer
-
Candida boidinii
1.1.1.284
dimer
2 * 39000, SDS-PAGE
Komagataella pastoris
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.284
30
-
-
Kloeckera sp.
1.1.1.284
35
-
-
Candida boidinii
1.1.1.284
47
-
-
Komagataella pastoris
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.1.1.284
20
65
20°C: about 60% of maximal activity, 65°C: about 40% of maximal activity
Komagataella pastoris
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.284
35
-
10 min, 50% loss of activity
Kloeckera sp.
1.1.1.284
52
-
10 min, 50% loss of activity
Candida boidinii
1.1.1.284
52
-
10 min, 50% loss of activity
Komagataella pastoris
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.233
-
glutathione
-
Komagataella pastoris
1.1.1.284
0.267
-
formaldehyde
-
Komagataella pastoris
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
7.9
-
-
Komagataella pastoris
1.1.1.284
8
-
-
Candida boidinii
1.1.1.284
8
-
-
Kloeckera sp.
1.1.1.284
8
-
-
Komagataella pastoris
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.284
6.4
9.3
50% of maximal activity at pH 6.4 and at pH 9.3
Komagataella pastoris
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.284
6.1
-
37°C, 10 min, 50% loss of activity
Komagataella pastoris
1.1.1.284
7
-
37°C, 10 min, about 20% loss of activity
Komagataella pastoris
1.1.1.284
8.5
-
37°C, 10 min, about 30% loss of activity
Komagataella pastoris
1.1.1.284
9.4
-
37°C, 10 min, about 50% loss of activity
Komagataella pastoris
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Candida boidinii
1.1.1.284
NAD+
-
Kloeckera sp.
1.1.1.284
NAD+
-
Komagataella pastoris
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Candida boidinii
1.1.1.284
NAD+
-
Kloeckera sp.
1.1.1.284
NAD+
-
Komagataella pastoris
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
Cd2+
1 mM, complete inhibition
Candida boidinii
1.1.1.284
Cd2+
1 mM, complete inhibition
Komagataella pastoris
1.1.1.284
citrate
1 mM, 24% inhibition
Komagataella pastoris
1.1.1.284
CN-
-
Candida boidinii
1.1.1.284
CN-
1 mM, 48% inhibition
Komagataella pastoris
1.1.1.284
Cu2+
-
Candida boidinii
1.1.1.284
Cu2+
1 mM, 76% inhibition
Komagataella pastoris
1.1.1.284
EDTA
1 mM, 12% inhibition
Komagataella pastoris
1.1.1.284
Hg2+
-
Candida boidinii
1.1.1.284
Hg2+
1 mM, 90% inhibition
Komagataella pastoris
1.1.1.284
NADH
-
Candida boidinii
1.1.1.284
NADH
product inhibition
Komagataella pastoris
1.1.1.284
NO2-
1 mM, 14% inhibition
Komagataella pastoris
1.1.1.284
Zn2+
-
Candida boidinii
1.1.1.284
Zn2+
1 mM, 28% inhibition
Komagataella pastoris
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.24
-
NAD+
-
Komagataella pastoris
1.1.1.284
0.43
-
formaldehyde
-
Komagataella pastoris
1.1.1.284
0.48
-
glutathione
-
Komagataella pastoris
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.284
39000
-
2 * 39000, SDS-PAGE
Komagataella pastoris
1.1.1.284
84000
-
gel filtration
Komagataella pastoris
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.284
-
Komagataella pastoris
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
10.2
-
-
Komagataella pastoris
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.284
4°C, in presence of glycerol, 70-80% recovery of activity after 7 days
Komagataella pastoris
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
-
80989
Komagataella pastoris
S-formylglutathione + NADH + H+
-
80989
Komagataella pastoris
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
80989
Candida boidinii
S-formylglutathione + NADH + H+
-
80989
Candida boidinii
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
80989
Kloeckera sp.
S-formylglutathione + NADH + H+
-
80989
Kloeckera sp.
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
80989
Komagataella pastoris IFP 206
S-formylglutathione + NADH + H+
-
80989
Komagataella pastoris IFP 206
?
3.1.2.12
S-formylglutathione + H2O
-
80989
Komagataella pastoris
glutathione + formate
-
80989
Komagataella pastoris
?
3.1.2.12
S-formylglutathione + H2O
-
80989
Komagataella pastoris IFP 206
glutathione + formate
-
80989
Komagataella pastoris IFP 206
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.284
dimer
-
Candida boidinii
1.1.1.284
dimer
2 * 39000, SDS-PAGE
Komagataella pastoris
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.284
30
-
-
Kloeckera sp.
1.1.1.284
35
-
-
Candida boidinii
1.1.1.284
47
-
-
Komagataella pastoris
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.1.1.284
20
65
20°C: about 60% of maximal activity, 65°C: about 40% of maximal activity
Komagataella pastoris
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.284
35
-
10 min, 50% loss of activity
Kloeckera sp.
1.1.1.284
52
-
10 min, 50% loss of activity
Candida boidinii
1.1.1.284
52
-
10 min, 50% loss of activity
Komagataella pastoris
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.233
-
glutathione
-
Komagataella pastoris
1.1.1.284
0.267
-
formaldehyde
-
Komagataella pastoris
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
7.9
-
-
Komagataella pastoris
1.1.1.284
8
-
-
Candida boidinii
1.1.1.284
8
-
-
Kloeckera sp.
1.1.1.284
8
-
-
Komagataella pastoris
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.284
6.4
9.3
50% of maximal activity at pH 6.4 and at pH 9.3
Komagataella pastoris
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.284
6.1
-
37°C, 10 min, 50% loss of activity
Komagataella pastoris
1.1.1.284
7
-
37°C, 10 min, about 20% loss of activity
Komagataella pastoris
1.1.1.284
8.5
-
37°C, 10 min, about 30% loss of activity
Komagataella pastoris
1.1.1.284
9.4
-
37°C, 10 min, about 50% loss of activity
Komagataella pastoris