BRENDA - Enzyme Database

Purificaton and characterization of S-formylglutathione hydrolase from a methanol-utilizing yeast, Kloeckera sp. No. 2201

Kato, N.; Sakazawa, C.; Nishizawa, T.; Tani, Y.; Yamada, H.; Biochim. Biophys. Acta 611, 323-332 (1980)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.12
5,5'-dithiobis(2-nitrobenzoate)
-
Kloeckera sp.
3.1.2.12
arsenite
-
Kloeckera sp.
3.1.2.12
CuSO4
-
Kloeckera sp.
3.1.2.12
FeSO4
-
Kloeckera sp.
3.1.2.12
HgCl2
-
Kloeckera sp.
3.1.2.12
iodoacetate
-
Kloeckera sp.
3.1.2.12
N-ethylmaleimide
-
Kloeckera sp.
3.1.2.12
p-chloromercuribenzoate
-
Kloeckera sp.
3.1.2.12
p-hydroxymercuribenzoate
-
Kloeckera sp.
3.1.2.12
Zn2+
-
Kloeckera sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.12
0.077
-
S-formylglutathione
-
Kloeckera sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.12
31000
-
2 * 31000 SDS-PAGE
Kloeckera sp.
3.1.2.12
58000
-
gel filtration
Kloeckera sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.2.12
S-formylglutathione + H2O
Candida boidinii
-
?
-
-
-
3.1.2.12
S-formylglutathione + H2O
Kloeckera sp.
involved in the oxidation of methanol by methylotrophic yeasts
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.2.12
Candida boidinii
-
-
-
3.1.2.12
Kloeckera sp.
-
No.2201, grown in presence of methanol
-
3.1.2.12
Ogataea angusta
-
-
-
3.1.2.12
Ogataea trehalophila
-
-
-
3.1.2.12
Torulopsis pinus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.2.12
-
Kloeckera sp.
Storage Stability
EC Number
Storage Stability
Organism
3.1.2.12
-20°C
Kloeckera sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.12
S-formylglutathione + H2O
-
80985
Candida boidinii
glutathione + formate
-
80985
Candida boidinii
?
3.1.2.12
S-formylglutathione + H2O
-
80985
Ogataea angusta
glutathione + formate
-
80985
Ogataea angusta
?
3.1.2.12
S-formylglutathione + H2O
-
80985
Kloeckera sp.
glutathione + formate
-
80985
Kloeckera sp.
?
3.1.2.12
S-formylglutathione + H2O
-
80985
Ogataea trehalophila
glutathione + formate
-
80985
Ogataea trehalophila
?
3.1.2.12
S-formylglutathione + H2O
-
80985
Torulopsis pinus
glutathione + formate
-
80985
Torulopsis pinus
?
3.1.2.12
S-formylglutathione + H2O
-
80985
Candida boidinii
?
-
-
-
-
3.1.2.12
S-formylglutathione + H2O
involved in the oxidation of methanol by methylotrophic yeasts
80985
Kloeckera sp.
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.1.2.12
dimer
-
Candida boidinii
3.1.2.12
dimer
2 * 31000 SDS-PAGE
Kloeckera sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.2.12
50
-
-
Kloeckera sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.2.12
45
-
-
Kloeckera sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.12
6.4
6.6
-
Kloeckera sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.2.12
7.2
7.6
5°C, stable for 4 d
Kloeckera sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.12
5,5'-dithiobis(2-nitrobenzoate)
-
Kloeckera sp.
3.1.2.12
arsenite
-
Kloeckera sp.
3.1.2.12
CuSO4
-
Kloeckera sp.
3.1.2.12
FeSO4
-
Kloeckera sp.
3.1.2.12
HgCl2
-
Kloeckera sp.
3.1.2.12
iodoacetate
-
Kloeckera sp.
3.1.2.12
N-ethylmaleimide
-
Kloeckera sp.
3.1.2.12
p-chloromercuribenzoate
-
Kloeckera sp.
3.1.2.12
p-hydroxymercuribenzoate
-
Kloeckera sp.
3.1.2.12
Zn2+
-
Kloeckera sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.12
0.077
-
S-formylglutathione
-
Kloeckera sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.12
31000
-
2 * 31000 SDS-PAGE
Kloeckera sp.
3.1.2.12
58000
-
gel filtration
Kloeckera sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.2.12
S-formylglutathione + H2O
Candida boidinii
-
?
-
-
-
3.1.2.12
S-formylglutathione + H2O
Kloeckera sp.
involved in the oxidation of methanol by methylotrophic yeasts
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.12
-
Kloeckera sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.2.12
-20°C
Kloeckera sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.12
S-formylglutathione + H2O
-
80985
Candida boidinii
glutathione + formate
-
80985
Candida boidinii
?
3.1.2.12
S-formylglutathione + H2O
-
80985
Ogataea angusta
glutathione + formate
-
80985
Ogataea angusta
?
3.1.2.12
S-formylglutathione + H2O
-
80985
Kloeckera sp.
glutathione + formate
-
80985
Kloeckera sp.
?
3.1.2.12
S-formylglutathione + H2O
-
80985
Ogataea trehalophila
glutathione + formate
-
80985
Ogataea trehalophila
?
3.1.2.12
S-formylglutathione + H2O
-
80985
Torulopsis pinus
glutathione + formate
-
80985
Torulopsis pinus
?
3.1.2.12
S-formylglutathione + H2O
-
80985
Candida boidinii
?
-
-
-
-
3.1.2.12
S-formylglutathione + H2O
involved in the oxidation of methanol by methylotrophic yeasts
80985
Kloeckera sp.
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.2.12
dimer
-
Candida boidinii
3.1.2.12
dimer
2 * 31000 SDS-PAGE
Kloeckera sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.2.12
50
-
-
Kloeckera sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.2.12
45
-
-
Kloeckera sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.12
6.4
6.6
-
Kloeckera sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.2.12
7.2
7.6
5°C, stable for 4 d
Kloeckera sp.