BRENDA - Enzyme Database

Purification of formaldehyde and formate dehydrogenases from pea seeds by affinity chromatography and S-formylglutathione as the intermediate of formaldehyde metabolism

Uotila, L.; Koivusalo, M.; Arch. Biochem. Biophys. 196, 33-45 (1979)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.1.1.284
enzyme is unstable in purified state
Pisum sativum
1.1.1.284
freezing almost completely destroys activity
Pisum sativum
1.17.1.9
rapid freezing in liquid nitrogen gives 75% recovery
Pisum sativum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
additional information
-
additional information
-
Pisum sativum
1.17.1.9
0.45
-
S-formylglutathione
-
Pisum sativum
1.17.1.9
2.1
-
formate
-
Pisum sativum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.284
42100
-
2 * 42100, SDS-PAGE
Pisum sativum
1.1.1.284
82300
-
gel filtration
Pisum sativum
1.17.1.9
42400
-
2 * 42400, SDS-PAGE
Pisum sativum
1.17.1.9
80300
-
gel filtration
Pisum sativum
1.17.1.9
82300
-
gel filtration
Pisum sativum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.284
Pisum sativum
-
-
-
1.17.1.9
Pisum sativum
-
-
-
3.1.2.12
Pisum sativum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.284
-
Pisum sativum
1.17.1.9
-
Pisum sativum
3.1.2.12
partial
Pisum sativum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.284
seed
-
Pisum sativum
-
1.17.1.9
seed
-
Pisum sativum
-
3.1.2.12
seed
-
Pisum sativum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
2.78
-
-
Pisum sativum
1.17.1.9
4.09
-
-
Pisum sativum
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.284
0°C, half-life is about 10 days
Pisum sativum
1.17.1.9
-70°C, without stabilizer, 85% loss of activity, 7% loss of activity in 33% glycerol solution
Pisum sativum
1.17.1.9
0°C, 50% loss of activity after 1 month
Pisum sativum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
-
80983
Pisum sativum
S-formylglutathione + NADH + H+
-
80983
Pisum sativum
-
1.1.1.284
glyoxal + glutathione + NAD+
35% of the activity with formaldehyde
80983
Pisum sativum
S-oxoacetylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
90% of the activity with formaldehyde
80983
Pisum sativum
S-pyruvylglutathione + NADH
-
-
-
?
1.17.1.9
ethyl formate + NAD+
-
80983
Pisum sativum
?
-
-
-
?
1.17.1.9
formate + NAD+
-
80983
Pisum sativum
CO2 + NADH
-
-
-
?
1.17.1.9
S-formylglutathione + NAD+
lower KM-value than for formate but maximal activity is only 5.5% of that of formate
80983
Pisum sativum
?
-
-
-
?
1.17.1.9
S-formylthioglycolate + NAD+
-
80983
Pisum sativum
?
-
-
-
?
3.1.2.12
S-formylglutathione + H2O
-
80983
Pisum sativum
glutathione + formate
-
80983
Pisum sativum
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.284
dimer
2 * 42100, SDS-PAGE
Pisum sativum
1.17.1.9
dimer
2 * 42400, SDS-PAGE
Pisum sativum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
6
-
below, reaction with S-formylglutathione + NADH
Pisum sativum
1.1.1.284
8
9
reaction with formaldehyde + NAD+ + glutathione
Pisum sativum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Pisum sativum
1.17.1.9
NAD+
-
Pisum sativum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Pisum sativum
1.17.1.9
NAD+
-
Pisum sativum
General Stability (protein specific)
EC Number
General Stability
Organism
1.1.1.284
enzyme is unstable in purified state
Pisum sativum
1.1.1.284
freezing almost completely destroys activity
Pisum sativum
1.17.1.9
rapid freezing in liquid nitrogen gives 75% recovery
Pisum sativum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
additional information
-
additional information
-
Pisum sativum
1.17.1.9
0.45
-
S-formylglutathione
-
Pisum sativum
1.17.1.9
2.1
-
formate
-
Pisum sativum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.284
42100
-
2 * 42100, SDS-PAGE
Pisum sativum
1.1.1.284
82300
-
gel filtration
Pisum sativum
1.17.1.9
42400
-
2 * 42400, SDS-PAGE
Pisum sativum
1.17.1.9
80300
-
gel filtration
Pisum sativum
1.17.1.9
82300
-
gel filtration
Pisum sativum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.284
-
Pisum sativum
1.17.1.9
-
Pisum sativum
3.1.2.12
partial
Pisum sativum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.284
seed
-
Pisum sativum
-
1.17.1.9
seed
-
Pisum sativum
-
3.1.2.12
seed
-
Pisum sativum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
2.78
-
-
Pisum sativum
1.17.1.9
4.09
-
-
Pisum sativum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.284
0°C, half-life is about 10 days
Pisum sativum
1.17.1.9
-70°C, without stabilizer, 85% loss of activity, 7% loss of activity in 33% glycerol solution
Pisum sativum
1.17.1.9
0°C, 50% loss of activity after 1 month
Pisum sativum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
-
80983
Pisum sativum
S-formylglutathione + NADH + H+
-
80983
Pisum sativum
-
1.1.1.284
glyoxal + glutathione + NAD+
35% of the activity with formaldehyde
80983
Pisum sativum
S-oxoacetylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
90% of the activity with formaldehyde
80983
Pisum sativum
S-pyruvylglutathione + NADH
-
-
-
?
1.17.1.9
ethyl formate + NAD+
-
80983
Pisum sativum
?
-
-
-
?
1.17.1.9
formate + NAD+
-
80983
Pisum sativum
CO2 + NADH
-
-
-
?
1.17.1.9
S-formylglutathione + NAD+
lower KM-value than for formate but maximal activity is only 5.5% of that of formate
80983
Pisum sativum
?
-
-
-
?
1.17.1.9
S-formylthioglycolate + NAD+
-
80983
Pisum sativum
?
-
-
-
?
3.1.2.12
S-formylglutathione + H2O
-
80983
Pisum sativum
glutathione + formate
-
80983
Pisum sativum
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.284
dimer
2 * 42100, SDS-PAGE
Pisum sativum
1.17.1.9
dimer
2 * 42400, SDS-PAGE
Pisum sativum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
6
-
below, reaction with S-formylglutathione + NADH
Pisum sativum
1.1.1.284
8
9
reaction with formaldehyde + NAD+ + glutathione
Pisum sativum