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Literature summary extracted from

  • Sly, W.S.; Stadtman, E.R.
    Formate metabolism. I. Formyl coenzyme A an intermediate in the formate-dependent decomposition of acetyl phosphate in Clostridium kluyveri (1963), J. Biol. Chem., 238, 2632-2638.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.2.10 dilution to 0.1 mg/ml, 50% loss of activity in 2 h Clostridium kluyveri
3.1.2.10 passage over Sephadex or dialysis for longer than 4 h, loss of activity up to 50% Clostridium kluyveri
3.1.2.10 reducing agents, crystalline bovine serum albumin have no stabilizing effect Clostridium kluyveri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.10 0.045
-
 formyl-CoA
-
 Clostridium kluyveri
3.1.2.10 0.08
-
 Formylpantetheine
-
 Clostridium kluyveri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.10 formyl-CoA + H2O Clostridium kluyveri formate-dependent decomposition of acetyl phosphate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.10 Clostridium aminobutyricum
-
-
-
3.1.2.10 Clostridium kluyveri
-
-
-
3.1.2.10 no activity in Clostridium cylindrosporum
-
-
-
3.1.2.10 no activity in Clostridium sticklandii
-
-
-
3.1.2.10 no activity in Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.10 partial Clostridium kluyveri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.10 90 100
-
 Clostridium kluyveri

Storage Stability

EC Number Storage Stability Organism
3.1.2.10 -20°C, stable for 2 months, in liquid nitrogen, pH 6.5, up to 6 months Clostridium kluyveri
3.1.2.10 0°C, concentrated solutions, 10 mg/ml, more than 1 week, partially purified Clostridium kluyveri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.10 formyl-CoA + H2O
-
 Clostridium kluyveri CoA + formate
-
 ?
3.1.2.10 formyl-CoA + H2O formate-dependent decomposition of acetyl phosphate Clostridium kluyveri ?
-
 ?
3.1.2.10 formylpantetheine + H2O
-
 Clostridium kluyveri pantetheine + formate
-
 ?
3.1.2.10 S-formylglutathione + H2O 3% of the rate observed with formylpantetheine Clostridium kluyveri glutathione + formate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.2.10 55
-
 5 min, 80% loss of activity Clostridium kluyveri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.10 6.8 7.1
-
 Clostridium kluyveri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.2.10 6 7.5 38% and 27% of optimal activity at pH 6.0 and pH 7.5 Clostridium kluyveri