BRENDA - Enzyme Database

Formate metabolism. I. Formyl coenzyme A an intermediate in the formate-dependent decomposition of acetyl phosphate in Clostridium kluyveri

Sly, W.S.; Stadtman, E.R.; J. Biol. Chem. 238, 2632-2638 (1963)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.1.2.10
dilution to 0.1 mg/ml, 50% loss of activity in 2 h
Clostridium kluyveri
3.1.2.10
passage over Sephadex or dialysis for longer than 4 h, loss of activity up to 50%
Clostridium kluyveri
3.1.2.10
reducing agents, crystalline bovine serum albumin have no stabilizing effect
Clostridium kluyveri
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.10
0.045
-
formyl-CoA
-
Clostridium kluyveri
3.1.2.10
0.08
-
Formylpantetheine
-
Clostridium kluyveri
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.2.10
formyl-CoA + H2O
Clostridium kluyveri
formate-dependent decomposition of acetyl phosphate
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.2.10
Clostridium aminobutyricum
-
-
-
3.1.2.10
Clostridium kluyveri
-
-
-
3.1.2.10
no activity in Clostridium cylindrosporum
-
-
-
3.1.2.10
no activity in Clostridium sticklandii
-
-
-
3.1.2.10
no activity in Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.2.10
partial
Clostridium kluyveri
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.10
90
100
-
Clostridium kluyveri
Storage Stability
EC Number
Storage Stability
Organism
3.1.2.10
-20°C, stable for 2 months, in liquid nitrogen, pH 6.5, up to 6 months
Clostridium kluyveri
3.1.2.10
0°C, concentrated solutions, 10 mg/ml, more than 1 week, partially purified
Clostridium kluyveri
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.10
formyl-CoA + H2O
-
80970
Clostridium kluyveri
CoA + formate
-
80970
Clostridium kluyveri
?
3.1.2.10
formyl-CoA + H2O
formate-dependent decomposition of acetyl phosphate
80970
Clostridium kluyveri
?
-
-
-
-
3.1.2.10
formylpantetheine + H2O
-
80970
Clostridium kluyveri
pantetheine + formate
-
80970
Clostridium kluyveri
?
3.1.2.10
S-formylglutathione + H2O
3% of the rate observed with formylpantetheine
80970
Clostridium kluyveri
glutathione + formate
-
80970
Clostridium kluyveri
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.2.10
55
-
5 min, 80% loss of activity
Clostridium kluyveri
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.10
6.8
7.1
-
Clostridium kluyveri
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.2.10
6
7.5
38% and 27% of optimal activity at pH 6.0 and pH 7.5
Clostridium kluyveri
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.2.10
dilution to 0.1 mg/ml, 50% loss of activity in 2 h
Clostridium kluyveri
3.1.2.10
passage over Sephadex or dialysis for longer than 4 h, loss of activity up to 50%
Clostridium kluyveri
3.1.2.10
reducing agents, crystalline bovine serum albumin have no stabilizing effect
Clostridium kluyveri
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.10
0.045
-
formyl-CoA
-
Clostridium kluyveri
3.1.2.10
0.08
-
Formylpantetheine
-
Clostridium kluyveri
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.2.10
formyl-CoA + H2O
Clostridium kluyveri
formate-dependent decomposition of acetyl phosphate
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.10
partial
Clostridium kluyveri
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.2.10
90
100
-
Clostridium kluyveri
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.2.10
-20°C, stable for 2 months, in liquid nitrogen, pH 6.5, up to 6 months
Clostridium kluyveri
3.1.2.10
0°C, concentrated solutions, 10 mg/ml, more than 1 week, partially purified
Clostridium kluyveri
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.10
formyl-CoA + H2O
-
80970
Clostridium kluyveri
CoA + formate
-
80970
Clostridium kluyveri
?
3.1.2.10
formyl-CoA + H2O
formate-dependent decomposition of acetyl phosphate
80970
Clostridium kluyveri
?
-
-
-
-
3.1.2.10
formylpantetheine + H2O
-
80970
Clostridium kluyveri
pantetheine + formate
-
80970
Clostridium kluyveri
?
3.1.2.10
S-formylglutathione + H2O
3% of the rate observed with formylpantetheine
80970
Clostridium kluyveri
glutathione + formate
-
80970
Clostridium kluyveri
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.2.10
55
-
5 min, 80% loss of activity
Clostridium kluyveri
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.10
6.8
7.1
-
Clostridium kluyveri
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.2.10
6
7.5
38% and 27% of optimal activity at pH 6.0 and pH 7.5
Clostridium kluyveri