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Literature summary extracted from
- The purification and characterization of a phospholipase A2 activity from the 106000 x g pellet (microsomal fraction) of bovine brain acting on phosphatidylinositol (1981), Can. J. Biochem., 60, 108-117.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.52 | octyl glucoside | stimulation | Bos taurus |  |
| 3.1.1.52 | Triton X-100 | stimulation | Bos taurus | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.1.52 | glycerol, asolecithin, 2-mecaptoethanol stabilize | Bos taurus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.52 | cetyltrimethylammonium bromide | - |
Bos taurus | |
| 3.1.1.52 | Mg2+ | - |
Bos taurus | |
| 3.1.1.52 | Mn2+ | - |
Bos taurus | |
| 3.1.1.52 | Zn2+ | - |
Bos taurus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.52 | 0.52 | - |
1-acyl-2-oleoyl-sn-glycerol-3-phosphoinositol | - |
Bos taurus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.52 | microsome | - |
Bos taurus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.52 | Ca2+ | activates | Bos taurus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.52 | 18300 | - |
x * 18521, amino acid analysis, x * 18300, SDS-PAGE | Bos taurus |
| 3.1.1.52 | 18520 | - |
amino acid analysis | Bos taurus |
| 3.1.1.52 | 18521 | - |
x * 18521, amino acid analysis, x * 18300, SDS-PAGE | Bos taurus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.52 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.52 | - |
Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.52 | brain | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.52 | 0.742 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.52 | 1-acyl-2-oleoyl-sn-glycerol-3-phosphoinositol + H2O | - |
Bos taurus | 1-acylglycerophosphoinositol + oleic acid | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.52 | ? | x * 18521, amino acid analysis, x * 18300, SDS-PAGE | Bos taurus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.52 | 70 | - |
stable up to | Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.52 | 7.4 | - |
- |
Bos taurus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.52 | 6 | 8 | - |
Bos taurus |
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Release 2023.1 (January 2023)
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