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Literature summary extracted from

  • Maya, H.; Aoki, J.; Kato, H.; Ishii, J.; Hino, S.; Arai, H.; Inoue, K.
    Biochemical characterization of various catalytic complexes of the brain platelet-activating factor acetylhydrolase (1999), J. Biol. Chem., 274, 31827-31832.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.47 medicine the gene for the beta subunit of brain enzyme isoform I is identical to a causative gene for Miller-Dieker lissencephaly Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.47 brain isoform I, expression of alpha1 and alpha2 subunits in Escherichia coli, expression of the beta subunit in Sf9 cells Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.47 H149R brain isoform I, beta subunit, mutant has no ability to associate with the catalytic alpha-complexes Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.47 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.47 brain
-
Bos taurus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.47 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O i.e. PAF Bos taurus 1-O-alkyl-sn-glycero-3-phosphocholine + acetate
-
?
3.1.1.47 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O preferred substrate for alpha2/alpha2 homodimer of brain isoform I Bos taurus 1-O-alkyl-sn-glycero-3-phosphocholine + acetate
-
?
3.1.1.47 1-O-alkyl-2-acetyl-sn-glycero-3-phosphoric acid + H2O preferred substrate for the alpha1/alpha1 homodimer and the alpha1/alpha2 heterodimer Bos taurus 1-O-alkyl-sn-glycero-3-phosphoric acid + acetate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.47 trimer the enzyme consists of two catalytic subunits alpha1 and alpha2 which form a catalytic dimer and a non-catalytic subunit beta. In addition the enzyme can aggregate to homodimers consisting of alpha1/alpha1 or alpha2/alpha2. The three forms differ in substrate specificity. The beta subunit accelerates the activity of the alpha2/alpha2 homodimer, suppresses the activity of the alpha1/alpha1 homodimer and has little influence on the activity of the alpha1/alpha2 heterodimer Bos taurus