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Literature summary extracted from

  • MacNaugthan, M.D.; Daugulis, A.J.
    Importance of enzyme and solvent physical properties for the biocompatibility relationship of alpha-amino acid ester hydrolase (1993), Enzyme Microb. Technol., 15, 114-119.
    View publication on PubMed

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.43 1,1,2-trichloroethane 50% v/v solution: 80% of maximal activity in hydrolysis of cephalexin Acetobacter pasteurianus
3.1.1.43 2,3-butanediol 50% v/v solution: 31% of maximal activity in hydrolysis of cephalexin, 44% of maximal activity in synthesis of cephalexin Acetobacter pasteurianus
3.1.1.43 2-heptanone 50% v/v solution: 105% of maximal activity in hydrolysis of cephalexin, 37% of maximal activity in synthesis of cephalexin Acetobacter pasteurianus
3.1.1.43 2-octanone 50% v/v solution: 101% of maximal activity in hydrolysis of cephalexin, 101% of maximal activity in synthesis of cephalexin Acetobacter pasteurianus
3.1.1.43 acetophenone 50% v/v solution: 57% of maximal activity in hydrolysis of cephalexin Acetobacter pasteurianus
3.1.1.43 butyl acetate 50% v/v solution: 106% of maximal activity in hydrolysis of cephalexin Acetobacter pasteurianus
3.1.1.43 chlorobenzene 50% v/v solution: 71% of maximal activity in hydrolysis of cephalexin Acetobacter pasteurianus
3.1.1.43 chloroform 50% v/v solution: 86% of maximal activity in hydrolysis of cephalexin Acetobacter pasteurianus
3.1.1.43 dibutyl ether 50% v/v solution: 113% of maximal activity in hydrolysis of cephalexin, 111% of maximal activity in synthesis of cephalexin Acetobacter pasteurianus
3.1.1.43 Ethyl acetate 50% v/v solution: 101% of maximal activity in hydrolysis of cephalexin, 82% of maximal activity in synthesis of cephalexin Acetobacter pasteurianus
3.1.1.43 heptane 50% v/v solution: 102% of maximal activity in hydrolysis of cephalexin, 117% of maximal activity in synthesis of cephalexin Acetobacter pasteurianus
3.1.1.43 hexadecane 50% v/v solution: 126% of maximal activity in hydrolysis of cephalexin Acetobacter pasteurianus
3.1.1.43 isopropanol 50% v/v solution: 18% of maximal activity in hydrolysis of cephalexin, 28% of maximal activity in synthesis of cephalexin Acetobacter pasteurianus
3.1.1.43 isopropyl acetate 50% v/v solution: 91% of maximal activity in hydrolysis of cephalexin, 100% of maximal activity in synthesis of cephalexin Acetobacter pasteurianus
3.1.1.43 Methanol 50% v/v solution: 13% of maximal activity in hydrolysis of cephalexin, 29% of maximal activity in synthesis of cephalexin Acetobacter pasteurianus
3.1.1.43 n-Butanol 50% v/v solution: 65% of maximal activity in hydrolysis of cephalexin, 47% of maximal activity in synthesis of cephalexin Acetobacter pasteurianus
3.1.1.43 n-decanol 50% v/v solution: 99% of maximal activity in hydrolysis of cephalexin Acetobacter pasteurianus
3.1.1.43 n-heptanol 50% v/v solution: 111% of maximal activity in hydrolysis of cephalexin, 115% of maximal activity in synthesis of cephalexin Acetobacter pasteurianus
3.1.1.43 n-nonanol 50% v/v solution: 102% of maximal activity in hydrolysis of cephalexin, 102% of maximal activity in synthesis of cephalexin Acetobacter pasteurianus
3.1.1.43 n-octanol 50% v/v solution: 110% of maximal activity in hydrolysis of cephalexin, 119% of maximal activity in synthesis of cephalexin Acetobacter pasteurianus
3.1.1.43 n-pentanol 50% v/v solution: 84% of maximal activity in hydrolysis of cephalexin, 73% of maximal activity in synthesis of cephalexin Acetobacter pasteurianus
3.1.1.43 phenoxy ethanol 50% v/v solution: 62% of maximal activity in hydrolysis of cephalexin Acetobacter pasteurianus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.43 Acetobacter pasteurianus
-
 ATCC 9325
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.43 cephalexin + H2O
-
 Acetobacter pasteurianus 7-amino-3-deacetoxycephalosporanic acid + phenylglycine
-
 r
3.1.1.43 D-alpha-phenylglycine methyl ester + 7-amino-3-deacetoxycephalosporanic acid
-
 Acetobacter pasteurianus methanol + cephalexin
-
 r