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Literature summary extracted from
- Studies on lichen enzymes. Purification and properties of an orsellinate depside hydrolase obtained from Lasallia pustulata (1971), Eur. J. Biochem., 22, 153-157.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.40 | diisopropylfluorophosphate | - |
Lasallia pustulata |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.40 | 0.056 | - |
Lecanoric acid | - |
Lasallia pustulata | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.40 | 42000 | - |
gel filtration, ultracentrifugation, SDS-PAGE | Lasallia pustulata |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.40 | Lasallia pustulata | - |
lichen | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.40 | - |
Lasallia pustulata |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.40 | 1300 | - |
- |
Lasallia pustulata |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.1.40 | frozen, 6 months, no loss of activity | Lasallia pustulata |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.40 | erythrin + H2O | - |
Lasallia pustulata | ? | - |
? | |
| 3.1.1.40 | evernic acid + H2O | - |
Lasallia pustulata | orsellinic acid + everninic acid | - |
? | |
| 3.1.1.40 | gyrophoric acid + H2O | - |
Lasallia pustulata | orsellinic acid | - |
? | |
| 3.1.1.40 | lecanoric acid + H2O | i.e. orsellinate depside | Lasallia pustulata | orsellinic acid | - |
? | |
| 3.1.1.40 | methyllecanorate + H2O | - |
Lasallia pustulata | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.40 | monomer | 1 * 42000, SDS-PAGE | Lasallia pustulata |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.40 | 25 | - |
stable for 4 days | Lasallia pustulata |
| 3.1.1.40 | 57 | - |
60 min stable, loss of activity if incubated longer | Lasallia pustulata |
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Release 2023.1 (January 2023)
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