BRENDA - Enzyme Database

Characterization of lysosomal acid lipase purified from rabbit liver

Imanaka, T.; Amanuma-Muto, K.; Ohkuma, S.; Takano, T.; J. Biochem. 96, 1089-1101 (1984) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.3
Cu2+
-
Oryctolagus cuniculus
3.1.1.3
diethyl dicarbonate
-
Oryctolagus cuniculus
3.1.1.3
diisopropyl fluorophosphate
weak
Oryctolagus cuniculus
3.1.1.3
p-bromophenacyl bromide
-
Oryctolagus cuniculus
3.1.1.3
p-chloromercuriphenyl sulfonic acid
-
Oryctolagus cuniculus
3.1.1.3
phenylmethylsulfonyl fluoride
weak
Oryctolagus cuniculus
3.1.1.13
Cu2+
strong inhibition at 2 mM
Oryctolagus cuniculus
3.1.1.13
diethyldicarbonate
complete inactivation at 2 mM
Oryctolagus cuniculus
3.1.1.13
diisopropylfluorophosphate
30% inhibition at 0.2 mM
Oryctolagus cuniculus
3.1.1.13
p-bromophenacylbromide
strong inhibition at 0.2 mM
Oryctolagus cuniculus
3.1.1.13
p-chloromercuriphenyl sulfonic acid
0.2 mM strongly inhibitory
Oryctolagus cuniculus
3.1.1.13
Phenylmethylsulfonylfluoride
30% inhibition at 0.2 mM
Oryctolagus cuniculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.3
0.03
-
4-methylumbelliferyl oleate
-
Oryctolagus cuniculus
3.1.1.3
0.04
-
cholesterol oleate
-
Oryctolagus cuniculus
3.1.1.3
0.05
-
triolein
-
Oryctolagus cuniculus
3.1.1.3
0.07
-
diolein
-
Oryctolagus cuniculus
3.1.1.3
0.2
-
monoolein
-
Oryctolagus cuniculus
3.1.1.13
0.03
-
4-methylumbelliferyl oleate
-
Oryctolagus cuniculus
3.1.1.13
0.04
-
cholesteryl oleate
-
Oryctolagus cuniculus
3.1.1.13
0.05
-
triolein
-
Oryctolagus cuniculus
3.1.1.13
0.07
-
diolein
-
Oryctolagus cuniculus
3.1.1.13
0.2
-
monoolein
-
Oryctolagus cuniculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.3
lysosome
-
Oryctolagus cuniculus
5764
-
3.1.1.13
lysosome
-
Oryctolagus cuniculus
5764
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.3
40000
-
gel filtration
Oryctolagus cuniculus
3.1.1.3
42000
-
1 * 42000, SDS-PAGE
Oryctolagus cuniculus
3.1.1.13
40000
-
gel filtration
Oryctolagus cuniculus
3.1.1.13
42000
-
SDS-PAGE
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.1.1.3
additional information
Oryctolagus cuniculus
the enzyme is involved in the hydrolysis of both acylglycerols and cholesterol esters in lysosomes
?
-
-
?
3.1.1.13
acylglycerols + H2O
Oryctolagus cuniculus
-
? + fatty acids
-
Oryctolagus cuniculus
?
3.1.1.13
cholesteryl esters + H2O
Oryctolagus cuniculus
-
cholesterol + fatty acid
-
Oryctolagus cuniculus
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.1.1.3
Oryctolagus cuniculus
-
-
-
3.1.1.13
Oryctolagus cuniculus
-
rabbit
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.1.1.3
side-chain modification
hydrophobic glycoprotein with variations in its carbohydrate chains
Oryctolagus cuniculus
3.1.1.13
side-chain modification
glycoprotein
Oryctolagus cuniculus
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.1.1.3
-
Oryctolagus cuniculus
3.1.1.13
25000fold to homogeneity, chromatography steps, preparative slab gel electrophoresis, affinity chromatography
Oryctolagus cuniculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.3
liver
-
Oryctolagus cuniculus
-
3.1.1.13
liver
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.3
216
-
-
Oryctolagus cuniculus
Storage Stability
EC Number
Storage Stability
Organism
3.1.1.3
-70°C, in presence of 40% v/v ethylene glycol and 5 mM mercaptoethanol, stable for at least 1 month
Oryctolagus cuniculus
3.1.1.13
-70°C, 40% ethylene glycol, 5 mM 2-mercaptoethanol, 1 month
Oryctolagus cuniculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.1.1.3
4-methylumbelliferyl ester + H2O
with fatty acids of different length in decreasing order, medium chain, long chains, short chains
80838
Oryctolagus cuniculus
4-methylumbelliferone + fatty acid
-
-
-
?
3.1.1.3
4-methylumbelliferyl oleate + H2O
-
80838
Oryctolagus cuniculus
4-methylumbelliferone + oleic acid
-
-
-
?
3.1.1.3
cholesterol oleate + H2O
-
80838
Oryctolagus cuniculus
cholesterol + oleic acid
-
-
-
?
3.1.1.3
diolein + H2O
-
80838
Oryctolagus cuniculus
oleic acid + ?
-
-
-
?
3.1.1.3
monoolein + H2O
-
80838
Oryctolagus cuniculus
oleic acid + glycerol
-
-
-
?
3.1.1.3
additional information
the enzyme is involved in the hydrolysis of both acylglycerols and cholesterol esters in lysosomes
80838
Oryctolagus cuniculus
?
-
-
-
?
3.1.1.3
trioleoylglycerol + H2O
-
80838
Oryctolagus cuniculus
oleic acid + ?
-
-
-
?
3.1.1.13
4-methylumbelliferyl oleate + H2O
-
80838
Oryctolagus cuniculus
4-methylumbelliferone + oleic acid
-
80838
Oryctolagus cuniculus
?
3.1.1.13
acylglycerols + H2O
-
80838
Oryctolagus cuniculus
? + fatty acids
-
80838
Oryctolagus cuniculus
?
3.1.1.13
cholesteryl esters + H2O
-
80838
Oryctolagus cuniculus
cholesterol + fatty acid
-
80838
Oryctolagus cuniculus
?
3.1.1.13
cholesteryl oleate + H2O
-
80838
Oryctolagus cuniculus
cholesterol + oleic acid
-
80838
Oryctolagus cuniculus
?
3.1.1.13
diolein + H2O
-
80838
Oryctolagus cuniculus
monooleoylglycerol + oleic acid
-
80838
Oryctolagus cuniculus
?
3.1.1.13
monoolein + H2O
-
80838
Oryctolagus cuniculus
glycerol + oleic acid
-
80838
Oryctolagus cuniculus
?
3.1.1.13
triolein + H2O
-
80838
Oryctolagus cuniculus
dioleoylglycerol + oleic acid
-
80838
Oryctolagus cuniculus
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.3
monomer
1 * 42000, SDS-PAGE
Oryctolagus cuniculus
3.1.1.13
monomer
1 * 40000-42000 SDS-PAGE, gel filtration
Oryctolagus cuniculus
Synonyms
EC Number
Synonyms
Commentary
Organism
3.1.1.13
acid lipase
-
Oryctolagus cuniculus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.3
45
-
10 min, 50% inactivation
Oryctolagus cuniculus
3.1.1.13
45
-
50% inactivation
Oryctolagus cuniculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.3
4.3
-
hydrolysis of cholesterol oleate
Oryctolagus cuniculus
3.1.1.3
4.5
-
hydrolysis of triolein, diolein or monoolein
Oryctolagus cuniculus
3.1.1.3
5
-
hydrolysis of 4-methylumbelliferyl oleate
Oryctolagus cuniculus
3.1.1.13
4.3
-
for cholesteryl oleate
Oryctolagus cuniculus
3.1.1.13
4.5
-
hydrolysis of tri-di- and monoolein
Oryctolagus cuniculus
3.1.1.13
5
-
for 4-methyl-umbelliferyl oleate
Oryctolagus cuniculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.3
Cu2+
-
Oryctolagus cuniculus
3.1.1.3
diethyl dicarbonate
-
Oryctolagus cuniculus
3.1.1.3
diisopropyl fluorophosphate
weak
Oryctolagus cuniculus
3.1.1.3
p-bromophenacyl bromide
-
Oryctolagus cuniculus
3.1.1.3
p-chloromercuriphenyl sulfonic acid
-
Oryctolagus cuniculus
3.1.1.3
phenylmethylsulfonyl fluoride
weak
Oryctolagus cuniculus
3.1.1.13
Cu2+
strong inhibition at 2 mM
Oryctolagus cuniculus
3.1.1.13
diethyldicarbonate
complete inactivation at 2 mM
Oryctolagus cuniculus
3.1.1.13
diisopropylfluorophosphate
30% inhibition at 0.2 mM
Oryctolagus cuniculus
3.1.1.13
p-bromophenacylbromide
strong inhibition at 0.2 mM
Oryctolagus cuniculus
3.1.1.13
p-chloromercuriphenyl sulfonic acid
0.2 mM strongly inhibitory
Oryctolagus cuniculus
3.1.1.13
Phenylmethylsulfonylfluoride
30% inhibition at 0.2 mM
Oryctolagus cuniculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.3
0.03
-
4-methylumbelliferyl oleate
-
Oryctolagus cuniculus
3.1.1.3
0.04
-
cholesterol oleate
-
Oryctolagus cuniculus
3.1.1.3
0.05
-
triolein
-
Oryctolagus cuniculus
3.1.1.3
0.07
-
diolein
-
Oryctolagus cuniculus
3.1.1.3
0.2
-
monoolein
-
Oryctolagus cuniculus
3.1.1.13
0.03
-
4-methylumbelliferyl oleate
-
Oryctolagus cuniculus
3.1.1.13
0.04
-
cholesteryl oleate
-
Oryctolagus cuniculus
3.1.1.13
0.05
-
triolein
-
Oryctolagus cuniculus
3.1.1.13
0.07
-
diolein
-
Oryctolagus cuniculus
3.1.1.13
0.2
-
monoolein
-
Oryctolagus cuniculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.3
lysosome
-
Oryctolagus cuniculus
5764
-
3.1.1.13
lysosome
-
Oryctolagus cuniculus
5764
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.3
40000
-
gel filtration
Oryctolagus cuniculus
3.1.1.3
42000
-
1 * 42000, SDS-PAGE
Oryctolagus cuniculus
3.1.1.13
40000
-
gel filtration
Oryctolagus cuniculus
3.1.1.13
42000
-
SDS-PAGE
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.1.1.3
additional information
Oryctolagus cuniculus
the enzyme is involved in the hydrolysis of both acylglycerols and cholesterol esters in lysosomes
?
-
-
?
3.1.1.13
acylglycerols + H2O
Oryctolagus cuniculus
-
? + fatty acids
-
Oryctolagus cuniculus
?
3.1.1.13
cholesteryl esters + H2O
Oryctolagus cuniculus
-
cholesterol + fatty acid
-
Oryctolagus cuniculus
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.1.1.3
side-chain modification
hydrophobic glycoprotein with variations in its carbohydrate chains
Oryctolagus cuniculus
3.1.1.13
side-chain modification
glycoprotein
Oryctolagus cuniculus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.3
-
Oryctolagus cuniculus
3.1.1.13
25000fold to homogeneity, chromatography steps, preparative slab gel electrophoresis, affinity chromatography
Oryctolagus cuniculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.3
liver
-
Oryctolagus cuniculus
-
3.1.1.13
liver
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.3
216
-
-
Oryctolagus cuniculus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.1.3
-70°C, in presence of 40% v/v ethylene glycol and 5 mM mercaptoethanol, stable for at least 1 month
Oryctolagus cuniculus
3.1.1.13
-70°C, 40% ethylene glycol, 5 mM 2-mercaptoethanol, 1 month
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.1.1.3
4-methylumbelliferyl ester + H2O
with fatty acids of different length in decreasing order, medium chain, long chains, short chains
80838
Oryctolagus cuniculus
4-methylumbelliferone + fatty acid
-
-
-
?
3.1.1.3
4-methylumbelliferyl oleate + H2O
-
80838
Oryctolagus cuniculus
4-methylumbelliferone + oleic acid
-
-
-
?
3.1.1.3
cholesterol oleate + H2O
-
80838
Oryctolagus cuniculus
cholesterol + oleic acid
-
-
-
?
3.1.1.3
diolein + H2O
-
80838
Oryctolagus cuniculus
oleic acid + ?
-
-
-
?
3.1.1.3
monoolein + H2O
-
80838
Oryctolagus cuniculus
oleic acid + glycerol
-
-
-
?
3.1.1.3
additional information
the enzyme is involved in the hydrolysis of both acylglycerols and cholesterol esters in lysosomes
80838
Oryctolagus cuniculus
?
-
-
-
?
3.1.1.3
trioleoylglycerol + H2O
-
80838
Oryctolagus cuniculus
oleic acid + ?
-
-
-
?
3.1.1.13
4-methylumbelliferyl oleate + H2O
-
80838
Oryctolagus cuniculus
4-methylumbelliferone + oleic acid
-
80838
Oryctolagus cuniculus
?
3.1.1.13
acylglycerols + H2O
-
80838
Oryctolagus cuniculus
? + fatty acids
-
80838
Oryctolagus cuniculus
?
3.1.1.13
cholesteryl esters + H2O
-
80838
Oryctolagus cuniculus
cholesterol + fatty acid
-
80838
Oryctolagus cuniculus
?
3.1.1.13
cholesteryl oleate + H2O
-
80838
Oryctolagus cuniculus
cholesterol + oleic acid
-
80838
Oryctolagus cuniculus
?
3.1.1.13
diolein + H2O
-
80838
Oryctolagus cuniculus
monooleoylglycerol + oleic acid
-
80838
Oryctolagus cuniculus
?
3.1.1.13
monoolein + H2O
-
80838
Oryctolagus cuniculus
glycerol + oleic acid
-
80838
Oryctolagus cuniculus
?
3.1.1.13
triolein + H2O
-
80838
Oryctolagus cuniculus
dioleoylglycerol + oleic acid
-
80838
Oryctolagus cuniculus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.3
monomer
1 * 42000, SDS-PAGE
Oryctolagus cuniculus
3.1.1.13
monomer
1 * 40000-42000 SDS-PAGE, gel filtration
Oryctolagus cuniculus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.3
45
-
10 min, 50% inactivation
Oryctolagus cuniculus
3.1.1.13
45
-
50% inactivation
Oryctolagus cuniculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.3
4.3
-
hydrolysis of cholesterol oleate
Oryctolagus cuniculus
3.1.1.3
4.5
-
hydrolysis of triolein, diolein or monoolein
Oryctolagus cuniculus
3.1.1.3
5
-
hydrolysis of 4-methylumbelliferyl oleate
Oryctolagus cuniculus
3.1.1.13
4.3
-
for cholesteryl oleate
Oryctolagus cuniculus
3.1.1.13
4.5
-
hydrolysis of tri-di- and monoolein
Oryctolagus cuniculus
3.1.1.13
5
-
for 4-methyl-umbelliferyl oleate
Oryctolagus cuniculus