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Literature summary extracted from
- Characterization of lysosomal acid lipase purified from rabbit liver (1984), J. Biochem., 96, 1089-1101.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.3 | Cu2+ | - |
Oryctolagus cuniculus |  |
| 3.1.1.3 | diethyl dicarbonate | - |
Oryctolagus cuniculus | |
| 3.1.1.3 | diisopropyl fluorophosphate | weak | Oryctolagus cuniculus | |
| 3.1.1.3 | p-bromophenacyl bromide | - |
Oryctolagus cuniculus | |
| 3.1.1.3 | p-chloromercuriphenyl sulfonic acid | - |
Oryctolagus cuniculus | |
| 3.1.1.3 | phenylmethylsulfonyl fluoride | weak | Oryctolagus cuniculus | |
| 3.1.1.13 | Cu2+ | strong inhibition at 2 mM | Oryctolagus cuniculus | |
| 3.1.1.13 | diethyldicarbonate | complete inactivation at 2 mM | Oryctolagus cuniculus | |
| 3.1.1.13 | diisopropylfluorophosphate | 30% inhibition at 0.2 mM | Oryctolagus cuniculus | |
| 3.1.1.13 | p-bromophenacylbromide | strong inhibition at 0.2 mM | Oryctolagus cuniculus | |
| 3.1.1.13 | p-chloromercuriphenyl sulfonic acid | 0.2 mM strongly inhibitory | Oryctolagus cuniculus | |
| 3.1.1.13 | Phenylmethylsulfonylfluoride | 30% inhibition at 0.2 mM | Oryctolagus cuniculus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.3 | 0.03 | - |
4-methylumbelliferyl oleate | - |
Oryctolagus cuniculus | |
| 3.1.1.3 | 0.04 | - |
cholesterol oleate | - |
Oryctolagus cuniculus | |
| 3.1.1.3 | 0.05 | - |
triolein | - |
Oryctolagus cuniculus | |
| 3.1.1.3 | 0.07 | - |
diolein | - |
Oryctolagus cuniculus | |
| 3.1.1.3 | 0.2 | - |
monoolein | - |
Oryctolagus cuniculus | |
| 3.1.1.13 | 0.03 | - |
4-methylumbelliferyl oleate | - |
Oryctolagus cuniculus | |
| 3.1.1.13 | 0.04 | - |
cholesteryl oleate | - |
Oryctolagus cuniculus | |
| 3.1.1.13 | 0.05 | - |
triolein | - |
Oryctolagus cuniculus | |
| 3.1.1.13 | 0.07 | - |
diolein | - |
Oryctolagus cuniculus | |
| 3.1.1.13 | 0.2 | - |
monoolein | - |
Oryctolagus cuniculus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.3 | lysosome | - |
Oryctolagus cuniculus | 5764 | - |
| 3.1.1.13 | lysosome | - |
Oryctolagus cuniculus | 5764 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 40000 | - |
gel filtration | Oryctolagus cuniculus |
| 3.1.1.3 | 42000 | - |
1 * 42000, SDS-PAGE | Oryctolagus cuniculus |
| 3.1.1.13 | 40000 | - |
gel filtration | Oryctolagus cuniculus |
| 3.1.1.13 | 42000 | - |
SDS-PAGE | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.3 | additional information | Oryctolagus cuniculus | the enzyme is involved in the hydrolysis of both acylglycerols and cholesterol esters in lysosomes | ? | - |
? | |
| 3.1.1.13 | acylglycerols + H2O | Oryctolagus cuniculus | - |
? + fatty acids | - |
? | |
| 3.1.1.13 | cholesteryl esters + H2O | Oryctolagus cuniculus | - |
cholesterol + fatty acid | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.3 | Oryctolagus cuniculus | - |
- |
- |
| 3.1.1.13 | Oryctolagus cuniculus | - |
rabbit | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | side-chain modification | hydrophobic glycoprotein with variations in its carbohydrate chains | Oryctolagus cuniculus |
| 3.1.1.13 | side-chain modification | glycoprotein | Oryctolagus cuniculus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.3 | - |
Oryctolagus cuniculus |
| 3.1.1.13 | 25000fold to homogeneity, chromatography steps, preparative slab gel electrophoresis, affinity chromatography | Oryctolagus cuniculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.3 | liver | - |
Oryctolagus cuniculus | - |
| 3.1.1.13 | liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 216 | - |
- |
Oryctolagus cuniculus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.1.3 | -70°C, in presence of 40% v/v ethylene glycol and 5 mM mercaptoethanol, stable for at least 1 month | Oryctolagus cuniculus |
| 3.1.1.13 | -70°C, 40% ethylene glycol, 5 mM 2-mercaptoethanol, 1 month | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.3 | 4-methylumbelliferyl ester + H2O | with fatty acids of different length in decreasing order, medium chain, long chains, short chains | Oryctolagus cuniculus | 4-methylumbelliferone + fatty acid | - |
? | |
| 3.1.1.3 | 4-methylumbelliferyl oleate + H2O | - |
Oryctolagus cuniculus | 4-methylumbelliferone + oleic acid | - |
? | |
| 3.1.1.3 | cholesterol oleate + H2O | - |
Oryctolagus cuniculus | cholesterol + oleic acid | - |
? | |
| 3.1.1.3 | diolein + H2O | - |
Oryctolagus cuniculus | oleic acid + ? | - |
? | |
| 3.1.1.3 | monoolein + H2O | - |
Oryctolagus cuniculus | oleic acid + glycerol | - |
? | |
| 3.1.1.3 | additional information | the enzyme is involved in the hydrolysis of both acylglycerols and cholesterol esters in lysosomes | Oryctolagus cuniculus | ? | - |
? | |
| 3.1.1.3 | trioleoylglycerol + H2O | - |
Oryctolagus cuniculus | oleic acid + ? | - |
? | |
| 3.1.1.13 | 4-methylumbelliferyl oleate + H2O | - |
Oryctolagus cuniculus | 4-methylumbelliferone + oleic acid | - |
? | |
| 3.1.1.13 | acylglycerols + H2O | - |
Oryctolagus cuniculus | ? + fatty acids | - |
? | |
| 3.1.1.13 | cholesteryl esters + H2O | - |
Oryctolagus cuniculus | cholesterol + fatty acid | - |
? | |
| 3.1.1.13 | cholesteryl oleate + H2O | - |
Oryctolagus cuniculus | cholesterol + oleic acid | - |
? | |
| 3.1.1.13 | diolein + H2O | - |
Oryctolagus cuniculus | monooleoylglycerol + oleic acid | - |
? | |
| 3.1.1.13 | monoolein + H2O | - |
Oryctolagus cuniculus | glycerol + oleic acid | - |
? | |
| 3.1.1.13 | triolein + H2O | - |
Oryctolagus cuniculus | dioleoylglycerol + oleic acid | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | monomer | 1 * 42000, SDS-PAGE | Oryctolagus cuniculus |
| 3.1.1.13 | monomer | 1 * 40000-42000 SDS-PAGE, gel filtration | Oryctolagus cuniculus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.13 | acid lipase | - |
Oryctolagus cuniculus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 45 | - |
10 min, 50% inactivation | Oryctolagus cuniculus |
| 3.1.1.13 | 45 | - |
50% inactivation | Oryctolagus cuniculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 4.3 | - |
hydrolysis of cholesterol oleate | Oryctolagus cuniculus |
| 3.1.1.3 | 4.5 | - |
hydrolysis of triolein, diolein or monoolein | Oryctolagus cuniculus |
| 3.1.1.3 | 5 | - |
hydrolysis of 4-methylumbelliferyl oleate | Oryctolagus cuniculus |
| 3.1.1.13 | 4.3 | - |
for cholesteryl oleate | Oryctolagus cuniculus |
| 3.1.1.13 | 4.5 | - |
hydrolysis of tri-di- and monoolein | Oryctolagus cuniculus |
| 3.1.1.13 | 5 | - |
for 4-methyl-umbelliferyl oleate | Oryctolagus cuniculus |
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