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Literature summary extracted from
- Enzymes of vitamin B6 degradation. Purification and properties of 4- and 5-pyridoxolactonases (1986), J. Biol. Chem., 261, 15112-15114.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.27 | 5-Pyridoxolactone | - |
Pseudomonas sp. |  |
| 3.1.1.27 | HgCl2 | - |
Pseudomonas sp. | |
| 3.1.1.27 | p-chloromercuribenzoate | - |
Pseudomonas sp. | |
| 3.1.1.27 | Zn2+ | - |
Pseudomonas sp. | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.27 | 0.0059 | - |
4-Pyridoxolactone | - |
Pseudomonas sp. | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.27 | 28000 | - |
2 * 28000, SDS-PAGE | Pseudomonas sp. |
| 3.1.1.27 | 54000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.27 | 4-Pyridoxolactone + H2O | Pseudomonas sp. | vitamin B6 degradation | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.27 | Pseudomonas sp. | - |
MA-1 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.27 | - |
Pseudomonas sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.27 | 35.2 | - |
- |
Pseudomonas sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.27 | 4-pyridoxolactone + H2O | no hydrolysis of 5-pyridoxolactone | Pseudomonas sp. | 4-pyridoxate | - |
? | |
| 3.1.1.27 | 4-Pyridoxolactone + H2O | vitamin B6 degradation | Pseudomonas sp. | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.27 | dimer | 2 * 28000, SDS-PAGE | Pseudomonas sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.27 | 7 | - |
- |
Pseudomonas sp. |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.27 | 5.9 | 8.7 | 50% of maximal activity at pH 5.9 and pH 8.7 | Pseudomonas sp. |
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