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Literature summary extracted from

  • Shirakura, Y.; Fukui, T.; Tanio, T.; Nakayama, K.; Matsuno, R.; Tomita, K.
    An extracellular D(-)-3-hydroxybutyrate oligomer hydrolase from Alcaligenes faecalis (1983), Biochim. Biophys. Acta, 748, 331-339.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.22 1.3
-
 (R)-3-((R)-3-hydroxybutanoyloxy)11butanoate
-
 Alcaligenes faecalis
3.1.1.22 1.5
-
 (R)-3-((R)-3-hydroxybutanoyloxy)7butanoate
-
 Alcaligenes faecalis
3.1.1.22 4
-
 (R)-3-((R)-3-hydroxybutanoyloxy)4butanoate
-
 Alcaligenes faecalis
3.1.1.22 5.2
-
 (R)-3-((R)-3-hydroxybutanoyloxy)2butanoate
-
 Alcaligenes faecalis
3.1.1.22 11.1
-
 (R)-3-((R)-3-hydroxybutanoyloxy)3butanoate
-
 Alcaligenes faecalis
3.1.1.22 32.8
-
 (R)-3-((R)-3-hydroxybutanoyloxy)butanoate
-
 Alcaligenes faecalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.22 extracellular
-
 Alcaligenes faecalis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.22 7200
-
 1 * 7200, SDS-PAGE Alcaligenes faecalis
3.1.1.22 68000 74000 gel filtration, SDS-PAGE Alcaligenes faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)3butanoate Alcaligenes faecalis degradation of poly-(3-hydroxybutyrate) ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.22 Alcaligenes faecalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.22
-
 Alcaligenes faecalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.22 41.2
-
-
 Alcaligenes faecalis

Storage Stability

EC Number Storage Stability Organism
3.1.1.22 -20°C, pH 7.5, 1 year Alcaligenes faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)11butanoate + H2O exo-type hydrolysis from the carboxyl terminus Alcaligenes faecalis (R)-3-hydroxybutanoate
-
 ?
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O exo-type hydrolysis from the carboxyl terminus Alcaligenes faecalis (R)-3-hydroxybutanoate
-
 ?
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)3butanoate degradation of poly-(3-hydroxybutyrate) Alcaligenes faecalis ?
-
 ?
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)3butanoate + H2O exo-type hydrolysis from the carboxyl terminus Alcaligenes faecalis (R)-3-hydroxybutanoate
-
 ?
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)4butanoate + H2O exo-type hydrolysis from the carboxyl terminus Alcaligenes faecalis (R)-3-hydroxybutanoate
-
 ?
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)7butanoate + H2O exo-type hydrolysis from the carboxyl terminus Alcaligenes faecalis (R)-3-hydroxybutanoate
-
 ?
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O i.e. D-(-)-3-[hydroxybutuyryloxy]butyric acid Alcaligenes faecalis (R)-3-hydroxybutanoate
-
 ?
3.1.1.22 (R)-3-((S)-3-hydroxybutanoyloxy)butanoate + H2O i.e. D-(-)-3-[L-(+)-hydroxybutuyryloxy]butyric acid Alcaligenes faecalis (R)-3-hydroxybutanoate + (S)-3-hydroxybutanoate
-
 ?
3.1.1.22 (S)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O i.e. L-(+)-3-[D-(-)-hydroxybutuyryloxy]butyric acid Alcaligenes faecalis (R)-3-hydroxybutanoate + (S)-3-hydroxybutanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.22 monomer 1 * 7200, SDS-PAGE Alcaligenes faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.22 8.5
-
-
 Alcaligenes faecalis