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Literature summary extracted from

  • Khalyfa, A.; Kermasha, S.; Marsot, P.; Goetghebeur, M.
    Purification and characterization of chlorophyllase from alga Phaeodactylum tricornutum by preparative native electrophoresis (1995), Appl. Biochem. Biotechnol., 53, 11-27.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.14 dithiothreitol in combination with Mg2+ dithiothreitol can activate chlorophyllase-catalyzed chlorophyll hydrolysis Phaeodactylum tricornutum
3.1.1.14 Lipids plant membrane lipids, including phosphatidylserine, phosphatidylglycerol, and beta-carotene increase activity Phaeodactylum tricornutum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.14 diisopropyl fluorophosphate
-
 Phaeodactylum tricornutum
3.1.1.14 phytol
-
 Phaeodactylum tricornutum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.14 Mg2+ in combination with dithiothreitol Mg2+ can activate chlorophyllase-catalyzed chlorophyll hydrolysis Phaeodactylum tricornutum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.14 43000
-
 x * 43000 + x * 46000, SDS-PAGE Phaeodactylum tricornutum
3.1.1.14 46000
-
 x * 43000 + x * 46000, SDS-PAGE Phaeodactylum tricornutum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.14 Phaeodactylum tricornutum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.14 partial Phaeodactylum tricornutum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.14 additional information
-
-
 Phaeodactylum tricornutum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.14 chlorophyll a + H2O
-
 Phaeodactylum tricornutum chlorophyllide a + phytol
-
 ?
3.1.1.14 chlorophyll b + H2O
-
 Phaeodactylum tricornutum chlorophyllide b + phytol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.14 ? x * 43000 + x * 46000, SDS-PAGE Phaeodactylum tricornutum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.14 31
-
-
 Phaeodactylum tricornutum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.14 8
-
-
 Phaeodactylum tricornutum