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Literature summary extracted from

  • Holden, M.
    The breakdown of chlorophyll by chlorophyllase (1961), Biochem. J., 78, 359-364.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.14 acetone presence of a solvent such as acetone is essential for enzyme action Beta vulgaris

General Stability

EC Number General Stability Organism
3.1.1.14 60% loss of activity after dialysis against distilled water in the cold for 48 h Beta vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.14 additional information Beta vulgaris first stage in the enzymic breakdown of chlorophyll in vivo may be the removal of the phytol side chain by chlorophyllase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.14 Beta vulgaris
-
 var. saccharifera
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.14 partial Beta vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.14 leaf
-
 Beta vulgaris
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.14 additional information first stage in the enzymic breakdown of chlorophyll in vivo may be the removal of the phytol side chain by chlorophyllase Beta vulgaris ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.14 20 30
-
 Beta vulgaris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.14
-
 45 0°C: about 35% of maximal activity, 45°C: about 50% of maximal activity Beta vulgaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.14 20
-
 24 h, 30-50% loss of activity Beta vulgaris
3.1.1.14 45
-
 1 h, pH 7, citrate buffer, stable. Complete loss of activity in presence of 40% acetone Beta vulgaris
3.1.1.14 60
-
 10 min, 40% loss of activity Beta vulgaris
3.1.1.14 65
-
 destroyed within 10 min Beta vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.14 7.5 8 soluble enzyme Beta vulgaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.14 6.4 8.7 pH 6.4: about 70% of maximal activity, pH 8.7: about 80% of maximal activity, soluble enzyme Beta vulgaris

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.14 3
-
 rapid inactivation Beta vulgaris
3.1.1.14 6 8 0°C, loss of 2% of activity per day Beta vulgaris