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Literature summary extracted from

  • Deng, S.; Gottlieb, L.; Pan, B.; Supplee, J.; Wei, X.; Petersson, E.J.; Marmorstein, R.
    Molecular mechanism of N-terminal acetylation by the ternary NatC complex (2021), Structure, 29, 1094-1104.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.256 Inositol hexaphosphate Kd value 225 nM, binds tightly to NatC to stabilize the complex. Presence of inositol hexaphosphate increases the thermal stability of the NatC complex by 4.9°C Schizosaccharomyces pombe

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.256 cryoelectron microscopy structure of NatC with a NatE/C-type bisubstrate analog and inositol hexaphosphate. The presence of three subunits is a prerequisite for normal NatC acetylation activity in yeast. Inositol hexaphosphate binds tightly to NatC to stabilize the complex. Naa38 plays key structural and, likely, functional roles in NatC. The Naa35 auxiliary subunit makes extensive contacts with the protein N-terminal substrate binding loops of Naa30 to influence substrate recognition Schizosaccharomyces pombe

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.256 E109A mutation of subunit Naa30, decrease in kcat value, increase in Km value Schizosaccharomyces pombe
2.3.1.256 E109Q mutation of subunit Naa30, decrease in kcat value, strong increase in Km value Schizosaccharomyces pombe
2.3.1.256 E25A mutation of subunit Naa30, increase in kcat value, increase in Km value Schizosaccharomyces pombe
2.3.1.256 M74A mutation of subunit Naa30, decrease in kcat value, increase in Km value Schizosaccharomyces pombe
2.3.1.256 N114A mutation of subunit Naa30, decrease in kcat value, decrease in Km value Schizosaccharomyces pombe
2.3.1.256 S24A mutation of subunit Naa30, catalytic parameters similar to wild-type Schizosaccharomyces pombe
2.3.1.256 Y121A mutation of subunit Naa30, strong decrease in kcat value Schizosaccharomyces pombe
2.3.1.256 Y121F mutation of subunit Naa30, strong decrease in kcat value, decrease in Km value Schizosaccharomyces pombe
2.3.1.256 Y135A mutation of subunit Naa30,decrease in kcat value, decrease in Km value Schizosaccharomyces pombe
2.3.1.256 Y136A mutation of subunit Naa30, strong decrease in kcat value, strong decrease in Km value Schizosaccharomyces pombe
2.3.1.256 Y27A mutation of subunit Naa30, decrease in kcat value, decrease in Km value Schizosaccharomyces pombe
2.3.1.256 Y71A mutation of subunit Naa30, increase in kcat value, increase in Km value Schizosaccharomyces pombe
2.3.1.256 Y71F mutation of subunit Naa30, increase in Km value Schizosaccharomyces pombe

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.256 0.0007
-
 MLRFVTKRWGRPVGRRRRP mutant Y136A, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 0.0025
-
 MLRFVTKRWGRPVGRRRRP mutant Y27A, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 0.004
-
 MLRFVTKRWGRPVGRRRRP mutant Y135A, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 0.0044
-
 MLRFVTKRWGRPVGRRRRP mutant N114A, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 0.0053
-
 MLRFVTKRWGRPVGRRRRP mutant Y121F, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 0.0078
-
 MLRFVTKRWGRPVGRRRRP wild-type, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 0.0089
-
 MLRFVTKRWGRPVGRRRRP mutant S24A, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 0.0097
-
 MLRFVTKRWGRPVGRRRRP mutant Y121A, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 0.0107
-
 MLRFVTKRWGRPVGRRRRP mutant Y71A, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 0.0111
-
 MLRFVTKRWGRPVGRRRRP mutant E25A, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 0.0114
-
 MLRFVTKRWGRPVGRRRRP mutant M74A, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 0.0139
-
 MLRFVTKRWGRPVGRRRRP mutant Y71F, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 0.0721
-
 MLRFVTKRWGRPVGRRRRP mutant E109Q, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 0.152
-
 MLRFVTKRWGRPVGRRRRP mutant E109A, pH 7, 23°C Schizosaccharomyces pombe

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.256 Schizosaccharomyces pombe O74311 and Q9USY3 and O43080 O74311 i.e. catalytic subunit Naa30, Q9USY3 i.e. auxiliary subunit Naa35, O43080 i.e. auxiliary subunit Naa38
-
2.3.1.256 Schizosaccharomyces pombe 972 O74311 and Q9USY3 and O43080 O74311 i.e. catalytic subunit Naa30, Q9USY3 i.e. auxiliary subunit Naa35, O43080 i.e. auxiliary subunit Naa38
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.256 acetyl-CoA + MLRFVTKRWGRPVGRRRRP
-
 Schizosaccharomyces pombe Nalpha-acetyl-L-methionyl-LRFVTKRWGRPVGRRRRP + CoA
-
 ?
2.3.1.256 acetyl-CoA + MLRFVTKRWGRPVGRRRRP
-
 Schizosaccharomyces pombe 972 Nalpha-acetyl-L-methionyl-LRFVTKRWGRPVGRRRRP + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.256 MAK10
-
 Schizosaccharomyces pombe
2.3.1.256 MAK3
-
 Schizosaccharomyces pombe
2.3.1.256 MAK31
-
 Schizosaccharomyces pombe
2.3.1.256 NAA30
-
 Schizosaccharomyces pombe
2.3.1.256 NatC
-
 Schizosaccharomyces pombe
2.3.1.256 SPBC15D4.06
-
 Schizosaccharomyces pombe
2.3.1.256 SPBC1861.03
-
 Schizosaccharomyces pombe
2.3.1.256 SPBC947.03c
-
 Schizosaccharomyces pombe

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.256 0.2
-
 MLRFVTKRWGRPVGRRRRP mutant Y136A, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 1.8
-
 MLRFVTKRWGRPVGRRRRP mutant Y121A, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 1.8
-
 MLRFVTKRWGRPVGRRRRP mutant Y121F, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 2.4
-
 MLRFVTKRWGRPVGRRRRP mutant E109Q, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 4.1
-
 MLRFVTKRWGRPVGRRRRP mutant Y135A, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 8.7
-
 MLRFVTKRWGRPVGRRRRP mutant Y27A, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 10.4
-
 MLRFVTKRWGRPVGRRRRP mutant E109A, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 10.9
-
 MLRFVTKRWGRPVGRRRRP mutant N114A, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 13.3
-
 MLRFVTKRWGRPVGRRRRP mutant M74A, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 18.4
-
 MLRFVTKRWGRPVGRRRRP mutant Y71F, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 19.1
-
 MLRFVTKRWGRPVGRRRRP mutant S24A, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 19.7
-
 MLRFVTKRWGRPVGRRRRP wild-type, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 32.7
-
 MLRFVTKRWGRPVGRRRRP mutant Y71A, pH 7, 23°C Schizosaccharomyces pombe
2.3.1.256 33.6
-
 MLRFVTKRWGRPVGRRRRP mutant E25A, pH 7, 23°C Schizosaccharomyces pombe