Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Huang, M.H.; Wang, J.T.; Zhang, J.H.; Mao, X.L.; Peng, G.X.; Lin, X.; Lv, D.; Yuan, C.; Lin, H.; Wang, E.D.; Zhou, X.L.
    Mitochondrial RNA m3C methyltransferase METTL8 relies on an isoform-specific N-terminal extension and modifies multiple heterogenous tRNAs (2023), Sci. Bull. (Beijing), 68, 2094-2105.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.268 expressed in HEK-293T cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.268 D38A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
2.1.1.268 D49A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
2.1.1.268 H45A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
2.1.1.268 K41A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
2.1.1.268 N46A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
2.1.1.268 R34A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
2.1.1.268 S33A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
2.1.1.268 T37A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.268 additional information t6A37 inhibits N3-methylcytosine modification by the enzyme at position 32 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.268 mitochondrion
-
 Homo sapiens 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.268 Mg2+ 10 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.268 S-adenosyl-L-methionine + cytosine32 in tRNASer Homo sapiens
-
 S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer
-
 ?
2.1.1.268 S-adenosyl-L-methionine + cytosine32 in tRNAThr Homo sapiens
-
 S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNAThr
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.268 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.268 HiTrap Q column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.268 additional information isoform METTL8-Iso4 is inactive in N3-methylcytosine32 generation due to the lack of N-extension Homo sapiens ?
-
-
2.1.1.268 S-adenosyl-L-methionine + cytosine32 in tRNASer
-
 Homo sapiens S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer
-
 ?
2.1.1.268 S-adenosyl-L-methionine + cytosine32 in tRNAThr
-
 Homo sapiens S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNAThr
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.268 METTL8-Iso1 isoform Homo sapiens
2.1.1.268 METTL8-Iso4 inactive isoform Homo sapiens
2.1.1.268 RNA m3C32 methyltransferase
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.1.1.268 physiological function isoform METTL8-Iso1 substantially stimulates the aminoacylation activities of SARS2 and TARS2 in vitro, suggesting a functional connection between mitochondrial tRNA modification and charging Homo sapiens