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Literature summary extracted from

  • Abedeera, S.M.; Hawkins, C.M.; Abeysirigunawardena, S.C.
    RsmG forms stable complexes with premature small subunit rRNA during bacterial ribosome biogenesis (2020), RSC Adv., 10, 22361-22369.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.170 gene rsmG, recombinant overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.170 C172S/A14C site-directed mutagenesis Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.170 additional information Escherichia coli the pseudoknot mutant (PKMut) G505A/C507U for 5'-domain RNA is obtained by site-directed mutagenesis. All 5'-domain RNA constructs are extended at the 3'-end to base pair with a fluorophore-labelled complementary DNA oligomer and in vitro transcribed ?
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2.1.1.170 additional information Escherichia coli K12 the pseudoknot mutant (PKMut) G505A/C507U for 5'-domain RNA is obtained by site-directed mutagenesis. All 5'-domain RNA constructs are extended at the 3'-end to base pair with a fluorophore-labelled complementary DNA oligomer and in vitro transcribed ?
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2.1.1.170 S-adenosyl-L-methionine + guanine527 in 16S rRNA Escherichia coli
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 S-adenosyl-L-homocysteine + N7-methylguanine527 in 16S rRNA
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 ?
2.1.1.170 S-adenosyl-L-methionine + guanine527 in 16S rRNA Escherichia coli K12
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 S-adenosyl-L-homocysteine + N7-methylguanine527 in 16S rRNA
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.170 Escherichia coli P0A6U5
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2.1.1.170 Escherichia coli K12 P0A6U5
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-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.170 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.170 additional information the pseudoknot mutant (PKMut) G505A/C507U for 5'-domain RNA is obtained by site-directed mutagenesis. All 5'-domain RNA constructs are extended at the 3'-end to base pair with a fluorophore-labelled complementary DNA oligomer and in vitro transcribed Escherichia coli ?
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2.1.1.170 additional information RsmG enzyme binds to Escherichia coli small subunit precursor rRNAs. Ability of RsmG to bind various premature small subunit ribosomal RNAs with contrasting affinities, overview. Protein RsmG binds with approximately 15times higher affinity to premature 16S rRNA with the full leader sequence compared to that of mature 16S rRNA. Various r-proteins which bind to the 5'-domain influence RsmG binding. The binding cooperativity between RsmG and r-proteins is sensitive to the maturation status of premature small subunit rRNA. But neither the maturation of 16S rRNA nor the presence of various r-proteins significantly influence the methylation activity of RsmG Escherichia coli ?
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2.1.1.170 additional information the pseudoknot mutant (PKMut) G505A/C507U for 5'-domain RNA is obtained by site-directed mutagenesis. All 5'-domain RNA constructs are extended at the 3'-end to base pair with a fluorophore-labelled complementary DNA oligomer and in vitro transcribed Escherichia coli K12 ?
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2.1.1.170 additional information RsmG enzyme binds to Escherichia coli small subunit precursor rRNAs. Ability of RsmG to bind various premature small subunit ribosomal RNAs with contrasting affinities, overview. Protein RsmG binds with approximately 15times higher affinity to premature 16S rRNA with the full leader sequence compared to that of mature 16S rRNA. Various r-proteins which bind to the 5'-domain influence RsmG binding. The binding cooperativity between RsmG and r-proteins is sensitive to the maturation status of premature small subunit rRNA. But neither the maturation of 16S rRNA nor the presence of various r-proteins significantly influence the methylation activity of RsmG Escherichia coli K12 ?
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2.1.1.170 S-adenosyl-L-methionine + guanine527 in 16S rRNA
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 Escherichia coli S-adenosyl-L-homocysteine + N7-methylguanine527 in 16S rRNA
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 ?
2.1.1.170 S-adenosyl-L-methionine + guanine527 in 16S rRNA RsmG binds to 30S 5'-domain and methylates N7 of G527 that is located at the highly conserved 530 loop of 16S helix 18. Substrates rRNAs and are 5'-leader rRNA fragments Escherichia coli S-adenosyl-L-homocysteine + N7-methylguanine527 in 16S rRNA
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 ?
2.1.1.170 S-adenosyl-L-methionine + guanine527 in 16S rRNA
-
 Escherichia coli K12 S-adenosyl-L-homocysteine + N7-methylguanine527 in 16S rRNA
-
 ?
2.1.1.170 S-adenosyl-L-methionine + guanine527 in 16S rRNA RsmG binds to 30S 5'-domain and methylates N7 of G527 that is located at the highly conserved 530 loop of 16S helix 18. Substrates rRNAs and are 5'-leader rRNA fragments Escherichia coli K12 S-adenosyl-L-homocysteine + N7-methylguanine527 in 16S rRNA
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.170 ribosomal small subunit methyltransferase G
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 Escherichia coli
2.1.1.170 rsmG
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 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.170 37
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 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.170 7.6
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 assay at Escherichia coli

General Information

EC Number General Information Comment Organism
2.1.1.170 evolution the enzyme is part of a SAM-dependent methyltransferase superfamily Escherichia coli
2.1.1.170 additional information ribosomal assembly proteins differentially stabilize RsmG complexes formed by mature and precursor 30S 5'-domain rRNA, positive and negative cooperativity, respectively, independency of the Mg2+ concentration, overview RsmG preferably binds to a native-like helix 18 structure Escherichia coli
2.1.1.170 physiological function protein RsmG is a methyltransferase enzyme that is responsible for N7 methylation in G527 of 16S rRNA. The capability of RsmG to bind to premature small subunit rRNA and alter its binding preference to various RNA-protein complexes based on the maturation of rRNA indicates its ability to influence ribosome assembly Escherichia coli