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Literature summary extracted from

  • Tzertzinis, G.; Ganatra, M.B.; Ruse, C.; Taron, C.H.; Causey, B.; Wang, L.; Schildkraut, I.
    The AMP deaminase of the mollusk Helix pomatia is an unexpected member of the adenosine deaminase-related growth factor (ADGF) family (2023), PLoS One, 18, e0286435.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.6 expression in Pichia pastoris Helix pomatia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.6 0.025
-
 AMP pH 7.5, 25°C Helix pomatia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.6 60000
-
 PAGE, plus degradation products of 32000 and 27000 Da Helix pomatia

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.6 Helix pomatia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.6 opurified from foot muscle by Q sepharose column, HiTrapTM Heparin columns and HiTrapTM SP column Helix pomatia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.6 foot muscle
-
 Helix pomatia
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.6 125
-
 substrate AMP, purified native protein, pH 7.5, 25°C Helix pomatia
3.5.4.6 550
-
 substrate AMP, recombinant protein, pH 7.5, 25°C Helix pomatia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.6 adenosine + H2O 4% of the rate with AMP Helix pomatia inosine + NH3
-
 ?
3.5.4.6 ADP + H2O 83% of the rate with AMP Helix pomatia IDP + NH3
-
 ?
3.5.4.6 AMP + H2O
-
 Helix pomatia IMP + NH3
-
 ?
3.5.4.6 ATP + H2O 25% of the rate with AMP Helix pomatia ITP + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.6 monomer 1 * 60000, SDS-PAGE, 1 * 58676, calculated from sequence for recombinant protein Helix pomatia

Synonyms

EC Number Synonyms Comment Organism
3.5.4.6 AmpD
-
 Helix pomatia
3.5.4.6 contig 71391
-
 Helix pomatia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.6 42
-
 30 min, no loss of activity Helix pomatia
3.5.4.6 54
-
 melting temperature Helix pomatia
3.5.4.6 65
-
 30 min, more than 95% loss of activity Helix pomatia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.4.6 170
-
 AMP pH 7.5, 25°C Helix pomatia