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Literature summary extracted from

  • Satoh, K.; Kawakami, T.; Isobe, N.; Pasquier, L.; Tomita, H.; Zinn, M.; Matsumoto, K.
    Versatile aliphatic polyester biosynthesis system for producing random and block copolymers composed of 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates using the sequence-regulating polyhydroxyalkanoate synthase PhaCAR (2022), Microb. Cell Fact., 21, 84.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.304 additional information sequence-regulating polyhydroxyalkanoate synthase PhaCAR is a chimeric enzyme comprising PhaCs from Aeromonas caviae and Ralstonia eutropha (Cupriavidus necator). Generation and evaluation of an versatile aliphatic polyester biosynthesis system for producing random and block copolymers composed of 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates using the sequence-regulating polyhydroxyalkanoate synthase PhaCAR, overview Aeromonas caviae
2.3.1.304 additional information sequence-regulating polyhydroxyalkanoate synthase PhaCAR is a chimeric enzyme comprising PhaCs from Aeromonas caviae and Ralstonia eutropha (Cupriavidus necator). Generation and evaluation of an versatile aliphatic polyester biosynthesis system for producing random and block copolymers composed of 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates using the sequence-regulating polyhydroxyalkanoate synthase PhaCAR, overview Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.304 (3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n Aeromonas caviae
-
 CoA + poly[(R)-3-hydroxyalkanoate]n+1
-
 ?
2.3.1.304 (3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n Cupriavidus necator
-
 CoA + poly[(R)-3-hydroxyalkanoate]n+1
-
 ?
2.3.1.304 (3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n Cupriavidus necator DSM 428
-
 CoA + poly[(R)-3-hydroxyalkanoate]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.304 Aeromonas caviae O32471
-
-
2.3.1.304 Cupriavidus necator P23608
-
-
2.3.1.304 Cupriavidus necator DSM 428 P23608
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.304 (3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n
-
 Aeromonas caviae CoA + poly[(R)-3-hydroxyalkanoate]n+1
-
 ?
2.3.1.304 (3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n
-
 Cupriavidus necator CoA + poly[(R)-3-hydroxyalkanoate]n+1
-
 ?
2.3.1.304 (3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n
-
 Cupriavidus necator DSM 428 CoA + poly[(R)-3-hydroxyalkanoate]n+1
-
 ?
2.3.1.304 (R,S)-2-hydroxybutyryl-CoA + glycolate-CoA polymerization of GL-CoA and 3HB-CoA Aeromonas caviae CoA + poly[2-hydroxybutyrate (3HB)]-beta-poly[glycolate (GL)-ran-3HB]
-
 ?
2.3.1.304 (R,S)-3-hydroxybutyryl-CoA + 3-hydroxypropionate
-
 Aeromonas caviae poly(3-hydroxybutyrate-co-3-hydroxypropionate) + CoA
-
 ?
2.3.1.304 (R,S)-3-hydroxybutyryl-CoA + 3-hydroxypropionate
-
 Cupriavidus necator poly(3-hydroxybutyrate-co-3-hydroxypropionate) + CoA
-
 ?
2.3.1.304 (R,S)-3-hydroxybutyryl-CoA + 3-hydroxypropionate
-
 Cupriavidus necator DSM 428 poly(3-hydroxybutyrate-co-3-hydroxypropionate) + CoA
-
 ?
2.3.1.304 (R,S)-3-hydroxybutyryl-CoA + 4-hydroxy-2-methylbutyrate
-
 Aeromonas caviae poly(3-hydroxybutyrate-co-4-hydroxy-2-methylbutyrate) + CoA
-
 ?
2.3.1.304 (R,S)-3-hydroxybutyryl-CoA + 4-hydroxy-2-methylbutyrate
-
 Cupriavidus necator poly(3-hydroxybutyrate-co-4-hydroxy-2-methylbutyrate) + CoA
-
 ?
2.3.1.304 (R,S)-3-hydroxybutyryl-CoA + 4-hydroxy-2-methylbutyrate
-
 Cupriavidus necator DSM 428 poly(3-hydroxybutyrate-co-4-hydroxy-2-methylbutyrate) + CoA
-
 ?
2.3.1.304 (R,S)-3-hydroxybutyryl-CoA + 5-hydroxyvalerate
-
 Aeromonas caviae poly(3-hydroxybutyrate-co-5-hydroxyvalerate) + CoA
-
 ?
2.3.1.304 (R,S)-3-hydroxybutyryl-CoA + 5-hydroxyvalerate
-
 Cupriavidus necator poly(3-hydroxybutyrate-co-5-hydroxyvalerate) + CoA
-
 ?
2.3.1.304 (R,S)-3-hydroxybutyryl-CoA + 5-hydroxyvalerate
-
 Cupriavidus necator DSM 428 poly(3-hydroxybutyrate-co-5-hydroxyvalerate) + CoA
-
 ?
2.3.1.304 (R,S)-3-hydroxybutyryl-CoA + glycolate-CoA polymerization of GL-CoA and 3HB-CoA Cupriavidus necator CoA + poly[3-hydroxybutyrate (3HB)]-beta-poly[glycolate (GL)-ran-3HB]
-
 ?
2.3.1.304 (R,S)-3-hydroxypropionyl-CoA + 6-hydroxyhexanoate
-
 Aeromonas caviae poly(3-hydroxybutyrate-co-6-hydroxyhexanoate) + CoA
-
 ?
2.3.1.304 (R,S)-3-hydroxypropionyl-CoA + 6-hydroxyhexanoate
-
 Cupriavidus necator poly(3-hydroxybutyrate-co-6-hydroxyhexanoate) + CoA
-
 ?
2.3.1.304 (R,S)-3-hydroxypropionyl-CoA + 6-hydroxyhexanoate
-
 Cupriavidus necator DSM 428 poly(3-hydroxybutyrate-co-6-hydroxyhexanoate) + CoA
-
 ?
2.3.1.304 additional information synthesis of 3HB-based binary copolymers containing 3HP, 4H2MB, 5HV, and 6HHx as secondary monomer units by recombinant chimeric enzyme PhaCAR in Escherichia coli. PhaCAR can efficiently incorporate 2HB and GL units into polymers. Using this enzyme, PHA block copolymers, P(2HB)-b-(3HB), P(GL-ran-3HB)-b-P(3HB), and P(GL-co-3HBco-3HHx)-b-P(3HB-co-3HHx) are synthesized. 2HB-containing ternary copolymer production is conducted using 3HB, 3HP, and LMC HAs in combination. The enzyme shows preference of PhaCAR for 3HB and 3HP monomers. Substrate specificity of chimeric enzyme PhaCAR, detailed overview Aeromonas caviae ?
-
-
2.3.1.304 additional information synthesis of 3HB-based binary copolymers containing 3HP, 4H2MB, 5HV, and 6HHx as secondary monomer units by recombinant chimeric enzyme PhaCAR in Escherichia coli. PhaCAR can efficiently incorporate 2HB and GL units into polymers. Using this enzyme, PHA block copolymers, P(2HB)-b-(3HB), P(GL-ran-3HB)-b-P(3HB), and P(GL-co-3HBco-3HHx)-b-P(3HB-co-3HHx) are synthesized. 2HB-containing ternary copolymer production is conducted using 3HB, 3HP, and LMC HAs in combination. The enzyme shows preference of PhaCAR for 3HB and 3HP monomers. Substrate specificity of chimeric enzyme PhaCAR, detailed overview Cupriavidus necator ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.3.1.304 class I 2HA-incorporating PHA synthase
-
 Aeromonas caviae
2.3.1.304 class I 2HA-incorporating PHA synthase
-
 Cupriavidus necator
2.3.1.304 PhaCAR
-
 Aeromonas caviae
2.3.1.304 PhaCAR
-
 Cupriavidus necator
2.3.1.304 sequence-regulating polyhydroxyalkanoate synthase PhaCAR
-
 Aeromonas caviae
2.3.1.304 sequence-regulating polyhydroxyalkanoate synthase PhaCAR
-
 Cupriavidus necator

General Information

EC Number General Information Comment Organism
2.3.1.304 physiological function PhaCAR is a class I 2HA-incorporating PHA synthase. Class I PHA synthases can recognize unusual long-main-chain hydroxyalkanoates (LMC HAs, C4 or more in the main-chain) monomers, such as 4-hydroxybutyrate (4HB) and 5-hydroxyvalerate (5HV) Aeromonas caviae
2.3.1.304 physiological function PhaCAR is a class I 2HA-incorporating PHA synthase. Class I PHA synthases can recognize unusual long-main-chain hydroxyalkanoates (LMC HAs, C4 or more in the main-chain) monomers, such as 4-hydroxybutyrate (4HB) and 5-hydroxyvalerate (5HV) Cupriavidus necator