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Literature summary extracted from

  • Han, L.; Marcus, E.; Phizicky, E.M.
    A semi-quantitative pull-down assay to study tRNA substrate specificity of modification enzymes (2021), Methods Enzymol., 658, 359-377.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.268 S-adenosyl-L-methionine + cytosine32 in tRNASer Saccharomyces cerevisiae the enzyme modifies tRNASer species with anticodon sequences UGA, CGA, and GCU S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer
-
 ?
2.1.1.268 S-adenosyl-L-methionine + cytosine32 in tRNAThr Saccharomyces cerevisiae the enzyme modifies tRNAThr species with anticodon sequences CGU, UGU, and IGU S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNAThr
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.268 Saccharomyces cerevisiae Q08641
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-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.268 IgG Sepharose bead chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.268 additional information prior N6-isopentenyladenosine modification of tRNAThr at position A37 is important for N3-methylcytosine32 modification by the enzyme. Prior N6-isopentenyladenosine or threonylcarbamoyladenosine modification of tRNASer at position A37 is important for N3-methylcytosine32 modification by the enzyme Saccharomyces cerevisiae ?
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-
2.1.1.268 S-adenosyl-L-methionine + cytosine32 in tRNASer the enzyme modifies tRNASer species with anticodon sequences UGA, CGA, and GCU Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer
-
 ?
2.1.1.268 S-adenosyl-L-methionine + cytosine32 in tRNAThr the enzyme modifies tRNAThr species with anticodon sequences CGU, UGU, and IGU Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNAThr
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.268 m3C32 methyltransferase
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 Saccharomyces cerevisiae
2.1.1.268 Trm140
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 Saccharomyces cerevisiae