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Literature summary extracted from

  • Sartor, P.; Denkhaus, L.; Gerhardt, S.; Einsle, O.; Fetzner, S.
    Structural basis of O-methylation of (2-heptyl-)1-hydroxyquinolin-4(1H)-one and related compounds by the heterocyclic toxin methyltransferase Rv0560c of Mycobacterium tuberculosis (2021), J. Struct. Biol., 213, 107794.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.374 expressed in Escherichia coli Rosetta (DE3) pLysS cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.374 enzyme in complex with 4-hydroxyisoquinoline-1(2H)-one or 1-methoxyquinolin-4(1H)-one, hanging drop vapor diffusion method, using either 2.4 M Na-malonate, 0.6 % Jeffamine ED-2001 pH 7.0 or sodium malonate (2.0-2.5 M, pH 7.0 or pH 6.0) or 70% (w/v) tacsimate pH 7.0 Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.374 H139A the mutant shows severely reduced specific activity compared to the wild type enzyme Mycobacterium tuberculosis
2.1.1.374 T136A the mutant shows severely reduced specific activity compared to the wild type enzyme Mycobacterium tuberculosis
2.1.1.374 T136A/H139A the mutant shows severely specific reduced activity compared to the wild type enzyme Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.374 S-adenosyl-L-methionine + 2-heptyl-1-hydroxyquinolin-4(1H)-one Mycobacterium tuberculosis also known as 2-heptyl-4-hydroxyquinoline N-oxide S-adenosyl-L-homocysteine + 2-heptyl-1-methoxyquinolin-4(1H)-one
-
 ?
2.1.1.374 S-adenosyl-L-methionine + 2-heptyl-1-hydroxyquinolin-4(1H)-one Mycobacterium tuberculosis H37Rv also known as 2-heptyl-4-hydroxyquinoline N-oxide S-adenosyl-L-homocysteine + 2-heptyl-1-methoxyquinolin-4(1H)-one
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.374 Mycobacterium tuberculosis P9WKL5
-
-
2.1.1.374 Mycobacterium tuberculosis H37Rv P9WKL5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.374 Ni-NTA column chromatography, and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.374 S-adenosyl-L-methionine + 2-heptyl-1-hydroxyquinolin-4(1H)-one also known as 2-heptyl-4-hydroxyquinoline N-oxide Mycobacterium tuberculosis S-adenosyl-L-homocysteine + 2-heptyl-1-methoxyquinolin-4(1H)-one
-
 ?
2.1.1.374 S-adenosyl-L-methionine + 2-heptyl-1-hydroxyquinolin-4(1H)-one also known as 2-heptyl-4-hydroxyquinoline N-oxide Mycobacterium tuberculosis H37Rv S-adenosyl-L-homocysteine + 2-heptyl-1-methoxyquinolin-4(1H)-one
-
 ?
2.1.1.374 S-adenosyl-L-methionine + 4-hydroxyisoquinoline-1(2H)-one
-
 Mycobacterium tuberculosis S-adenosyl-L-homocysteine + 1-methoxyquinolin-4(1H)-one
-
 ?
2.1.1.374 S-adenosyl-L-methionine + 4-hydroxyisoquinoline-1(2H)-one
-
 Mycobacterium tuberculosis H37Rv S-adenosyl-L-homocysteine + 1-methoxyquinolin-4(1H)-one
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.374 monomer x-ray crystallography Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.1.1.374 heterocyclic toxin methyltransferase
-
 Mycobacterium tuberculosis
2.1.1.374 htm
-
 Mycobacterium tuberculosis
2.1.1.374 rv0560c
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.374 S-adenosyl-L-methionine dependent on Mycobacterium tuberculosis